... sensitivity. Observations: intensity of the CAGE TSSchip represents the activity of each TSS Having established the accuracy and sensitivity of the CAGE- TSSchip, we investigated several promoters of ... reflected the activity of each TSS, and so this technology may be useful in evaluating regulatory pathways. CAGE-TSSchip permits promoter-based expression profiling...
Ngày tải lên: 14/08/2014, 20:22
... purposes) Illustrations of the top gene networks at the 3 day time point of the time-dependent microarray analysisFigure 4 Illustrations of the top gene networks at the 3 day time point of the time-dependent ... the top gene networks at the 7 day time point of the time-dependent microarray analysisFigure 5 Illustrations of the top gene networks at the 7 day t...
Ngày tải lên: 12/08/2014, 14:20
Báo cáo y học: "Microarray gene expression profiling of osteoarthritic bone suggests altered bone remodelling, WNT and transforming growth factor-β/bone morphogenic protein signalling" pot
... consistency between the microarray and PCR detection of expression ratio differences for each of the genes analyzed, suggesting that the majority of the genes identified by the microarray are bona ... human primary subchondral osteoblasts [39]. In the present study, we used microarray analysis to survey comprehensively the expression levels of many thousands of gen...
Ngày tải lên: 09/08/2014, 10:21
Báo cáo y học: "Host gene expression profiling in influenza A virus-infected lung epithelial (A549) cells: a comparative analysis between highly pathogenic and modified H5N1 viruses" ppt
... Carlsbad, CA, USA) and purified by the RNeasy kit (Qiagen, Germany) following standard methodology. Amplification of RNA and indirect labeling of Cy-dye was done by Amino Allyl MessageAmp II aRNA amplification ... selectively taken for RT-PCR analysis. The expressions of these genes were found to be matching with the microarray analysis. Chakrabarti et al. Virology Journal 2010, 7...
Ngày tải lên: 12/08/2014, 01:21
Báo cáo y học: "Cell-specific microarray profiling experiments reveal a comprehensive picture of gene expression in the C. elegans nervous system" ppsx
... inhibit the Smad/Sno complex DAF-3/DAF-5, thereby promoting reproductive growth. The guanylyl cyclase DAF-11 drives expression of DAF-28 and DAF-7. During reproductive growth, the CYP2 cytochrome ... showing the serotonergic neuron NSM and two sensory neurons, AFD and ASI. For simplicity, only one of the two pairs of neurons is diagrammed. The pharynx is colored green a...
Ngày tải lên: 14/08/2014, 07:22
Báo cáo y học: "Genome-wide expression profiling and bioinformatics analysis of diurnally regulated genes in the mouse prefrontal cortex" pps
... Despite the liberal FDR threshold used in our study, some of these genes may serve as candidates for stud- ying the role of prefrontal cortex in the regulation of circadian rhythm, diurnal activity ... (in Figure 4, many cells in the rows for TFBS2 and TFBS7 are red). Therefore, the diurnal expression of many genes in these three clusters may be due to the transcriptiona...
Ngày tải lên: 14/08/2014, 08:20
Báo cáo y học: "Quantitative protein expression profiling reveals extensive post-transcriptional regulation and post-translational modifications in schizont-stage malaria parasites" doc
... Pool gel1 Cy3 Cy5 Cy2 gel2 Cy5 Cy3 Cy2 gel3 Cy3 Cy5 Cy2 gel4 Cy5 Cy3 Cy2 gel5 Cy3 Cy5 Cy2 gel6 Cy3 Cy5 Cy2 gel7 Cy5 Cy3 Cy2 gel8 Cy3 Cy5 Cy2 Each two-dimensional gel was loaded with 50 μg of a Cy3-labeled ... biologically significant. Of these, about one quarter (69 isoforms) change by more than threefold and 9% (24 isoforms) by more than fivefold (Figure 3a). Classifying these 278 expre...
Ngày tải lên: 14/08/2014, 21:20
Báo cáo y học: " Bronchiolar chemokine expression is different after single versus repeated cigarette smoke exposure" docx
... destruction of lung parenchyma. Since the pathology of COPD is that of a chronic inflammatory process, many studies have focused on identifying the inflammatory cell types and/or cytokines that play a ... pro-inflammatory cytokines, and that the kinetics of the response would differ depending on dura- tion of exposure and the cytokine. Accordingly, we exam- ined the exp...
Ngày tải lên: 12/08/2014, 15:21
Báo cáo y học: " Individual mRNA expression profiles reveal the effects of specific microRNAs Amit Arora and David AC Simpson" pot
... on the expression of their predicted targets (labeled 'mid'). The boxplots illustrate the significantly higher expression of miRNAs with low target gene expression. The scale of the y- axis ... effect on their target expression (labeled 'mid'). The box-plots show the copy number (y- axis) of the miRNAs in each group (x-axis) and illustrate...
Ngày tải lên: 14/08/2014, 08:21
Tài liệu Báo cáo Y học: A novel, inducible, citral lyase purified from spores of Penicillium digitatum docx
... [31], respectively. Citral lyase is a monomeric enzyme of 30 kDa, which is approximately the monomeric size of these hydratase/aldolase enzymes. All of these enzymes exhibit more then 75% of their maximum ... Lowry [15] using bovine serum albumin as the standard. RESULTS Induction of citral lyase Initially, the reproducibility of the results was hindered by variations in...
Ngày tải lên: 21/02/2014, 01:21