Báo cáo sinh học: "Tissue specific promoters improve specificity of AAV9 mediated transgene expression following intra-vascular gene delivery in neonatal mice" pptx

... paper Tissue specific promoters improve specificity of AAV9 mediated transgene expression following intra-vascular gene delivery in neonatal mice Christina A Pacak † , Yoshihisa Sakai † , Bijoy ... muscle following systemic vector administration. Importantly, these promoters provide an added layer of control over transgene activity following systemic g...

Ngày tải lên: 14/08/2014, 19:22

... as an insulin-binding protein [6]. Although the reported binding of IGFBP-7 to insulin awaits confirmation [7,8], it can compete with insulin for binding to the insulin receptor (InR) and inhibit ... IGFBPs, possibly accounting for the affinity of IGFBP-7 for insulin [6]. Interestingly, Imp-L2 has been shown to bind human insulin, IGF-I, IGF-II and proinsulin, and its homolog in the mot...

Ngày tải lên: 06/08/2014, 18:21

... Malaria Ini- tiative have significantly increased availability and access to medicines in some parts of the developing world. How- ever, these increases in the supply of medications are straining ... medications inventories accurately. In addition, increased supply of medicines often means increased opportunity for inappropriate use [1-3]. Inap- propriate patterns of drug use be...

Ngày tải lên: 18/06/2014, 17:20

... Inter- estingly, in the Achilles cells, although there was a reduc- tion in the intensity of staining indicating a reduction in cell number, on analysis there was no significant reduc- tion in ... pattern of response was inconsistent in the other markers. In the rat, the intensity of expression of CD90 was also higher in the PT cells as compared to the Achilles and tai...

Ngày tải lên: 20/06/2014, 01:20

... estimation package. In: Proceedings of an EC Symposium on Application of Mixed Linear Models in the Prediction of Genetic Merit in Pigs (E Groeneveld, ed) (in press) Kovac ... number of illegal points prior to reparameterization. Accordingly, run 1 finished in less than a 20th of the time converging to the best point, while run 2 a...

Ngày tải lên: 09/08/2014, 18:21

... proba- bility of containing the same number of chromosomes assum- ing a uniform random distribution of 40 spherical chromosomes of excluding volume with a radius of 10% of the nuclear volume in a spherical ... visualized single chromo- somes by fluorescence in situ hybridization (FISH) using chromosome -specific probes and analyzed their position in normal interphase cells...

Ngày tải lên: 09/08/2014, 20:20

... levels of transgene expression. From our experience shown on this study of using single-stranded rAAV2-TTR-RB15 vec- tor in mice, we knew it is necessary to generate an optimal vector to obtain successful ... a sequence-nonspecific interferon response [29] and the induction of gene activation of siRNA appears to be tran- sient. However, using synthetic 21 nucleotides targeted...

Ngày tải lên: 14/08/2014, 19:22

... design of new delivery strategies and biosafety. Integration into the host cell genome could produce inser- tional mutagenesis, which would have the potential of activating or inactivating genes. In ... its integrity [32]. In addition, the results provided by Ledwith et al. (2000), using different plasmid constructs suggest that the risk of integration of plasmid DNA vaccines f...

Ngày tải lên: 14/08/2014, 19:22

... involved in the uptake process, mice received a single dose of an intramuscular injection of 6- coumarin labeled microspheres containing pcDNA 3 - Hsp65/TDM, pcDNA 3 /TDM (microspheres containing ... of these important questions in more detail is required to assure the biosafety of microspheres allowing the advance of this technology to a clinical setting against tuberculosis or...

Ngày tải lên: 14/08/2014, 19:22

... higher transgene expression than FIV-WE54 in neonatal mice initially (1 and 3 weeks post-injection) and subsequently d isplayed similar levels of expression for the remaining 13 weeks of observation ... administered in vivo are pre- sented in Table 1. The University of Iowa Institutional Animal Care and Use Committee approved this study. Bioluminescence imaging Following...

Ngày tải lên: 14/08/2014, 19:22