Báo cáo sinh học: "Immediate transfection of patient-derived leukemia: a novel source for generating cell-based vaccines" ppt

... transfection of patient-derived leukemia: a novel source for generating cell-based vaccines Jill A Gershan, Bryon D Johnson, James Weber, Dennis W Schauer, Natalia Natalia, Stephanie Behnke, Karen Burns, ... non-nucleofected U2OS cells. Nuclear DNA was harvested and amplified from 3 separate samples, error bars indicate the standard deviation from the average of trip...

Ngày tải lên: 14/08/2014, 19:22

... Germany) in an ABI 7300 analyzer. Primers used for determining mRNA expression levels were as fol- lows: CD24 fwd 5’-TGC CTC GAC ACA CAT AAA CC- 3’, CD24 rev 5’-GTG ACC ATG CGA ACA AAA GA-3’; GAPDH ... 5’ -ACA CCC ACT CCT CCA CC T TT -3’, GAPDH rev 5 ’ -TGC TGT AGC CAA ATT CGT TG-3’. To compare and quantify different measurements a cellu- lar cDNA was used as standard and the amount was c...

Ngày tải lên: 18/06/2014, 19:20

... Diaz Munoz de la Espada VM, Garcia Alfonso P, Encina Garcia S, Izarzugaza Peron Y, Arranz Cozar JL, Hernandez Marin B, Perez Manga G: Management of gastric adenocarcinoma. Clin Transl Oncol 2007, 9:438-442. 3. ... Dar AA, Zaika A, Piazuelo MB, Correa P, Koyama T, Belkhiri A, Washington K, Castells A, Pera M, El-Rifai W: Frequent overexpression of Aurora Kinase A Holbrook et al. Journ...

Ngày tải lên: 18/06/2014, 22:20

... of Mathematics Education and the RINS, Gyeongsang National University, Chinju 660-701, Republic of Korea 3 Department of Information Management, Cheng Shiu University, Kaohsiung 833, Taiwan ∗ Corresponding ... Provisional PDF corresponds to the article as it appeared upon acceptance. Fully formatted PDF and full text (HTML) versions will be made available soon. Hierarchical convergence...

Ngày tải lên: 18/06/2014, 22:20

... contiguous habitat available for deer in that part of the Vosges mountains is calculated at approximately 209 animals, the actual population numbers at some 980 deer (a safe minimum ... area; the area of Vosgian habitat from which 85% of all parents of central individuals are derived is calculated as 129 126 ha. Only populations inhabi...

Ngày tải lên: 14/08/2014, 19:22

... treatment repeat and may allow sustained Epo secretion and constant patient coverage. Epo gene transfer has already been tested on normal animals and on anaemia animal models such as β-thalassemia ... more than one year period. Looking at indi- vidual data, it appears that the hematocrit can be main- tained at an almost constant level for each of the treated animal. This strategy allows t...

Ngày tải lên: 14/08/2014, 19:22

... GC -A receptor consists of an extracellular ligand-binding domain of approximately 441 amino acids (aa), a short membrane-spanning region (21 aa) and an intracellular portion (567 aa), containing ... Dr Ruey-Bing Yang (Academia Sinica, Taipei, Taiwan). References 1 Kuhn M (2003) Structure, regulation, and function of mammalian membrane guanylyl cyclase receptors, with a focus on gu...

Ngày tải lên: 29/03/2014, 09:20

... analysis gave basal area (at breast height) and bole vol- ume increments for each year. Disks were anal- ysed at 1 year intervals along the bole. For each disk, annual radial ... and leaf area of balsam fir. Can J For Res 22, 1684-1693 Dong PH, Laar A Van, Kramer H (1989) [A model for research on forest decline] in German. Allg F...

Ngày tải lên: 08/08/2014, 19:21

... that the complete autoantibody profile is important for a careful exami- nation of patients with rheumatic diseases and to access all their clinical features. Frank and colleagues analyzed sera ... Germany) for providing clinically defined sera, Mark L Fritzler (University of Calgary, Canada) for technical assistance with the addressable laser bead immunoassay and Wilma Vree Egb...

Ngày tải lên: 09/08/2014, 06:22

... study was to investigate the diagnostic accuracy of a novel and commercially available ELISA system for the detection of antibodies against a modi- fied citrullinated vimentin (anti-MCV) in a large ... testing Anti-MCV and anti-CCP2 testing was performed in an investi- gator-blinded fashion. Anti-CCP2 antibody reactivity was tested using a commercially available automated ELIS...

Ngày tải lên: 09/08/2014, 08:22