Báo cáo sinh học: "In vivo gene targeting of IL-3 into immature hematopoietic cells through CD117 receptor mediated antibody gene delivery" pot

... Vaccines and Therapy Open Access Research In vivo gene targeting of IL-3 into immature hematopoietic cells through CD117 receptor mediated antibody gene delivery Alain Chapel* 1,2 , Olivier Deas 3,4 , ... clearly indicate that antibody -mediated endocytosis gene transfer allows the expression of the IL-3 transgene into hematopoietic immature cells...

Ngày tải lên: 14/08/2014, 19:22

... vectors in mammalian cells. Finally, this study provides the proof of principle of baculovirus mediated gene transfer to endothelial cells in vivo and suggest the possibility of using this recombinant baculovirus ... to transfer genes for its expression into a wide variety of mammalian cells [7]. Since the first studies made by two different groups, showing the ability o...

Ngày tải lên: 18/06/2014, 18:20

... Tn-368 and Ld-652Y cells [4,5]. Hz-2V replication in vivo in the epithelial cells of agonadal female oviduct tissue has been described previously by Rallis and Burand [8]. The level of detectable ... fertile, asymptomatic carrier of Hz-2V. Conclusions The evolution of Hz-2V infection in H. zea has resulted in the ability of the virus to produce two different types of infecti...

Ngày tải lên: 18/06/2014, 22:20

... vectors in mammalian cells. Finally, this study provides the proof of principle of baculovirus mediated gene transfer to endothelial cells in vivo and suggest the possibility of using this recombinant baculovirus ... presence of 5 mM butyrate. Analysis by flow cytometry 48 h after transduction with BacFLT-GFP (MOI of 100) showed a large number of GFP+ cells (60.34%, n S...

Ngày tải lên: 20/06/2014, 01:20

... transglutaminase. Determination of the DCA-binding sites of histone H2B Because the carboxamide group of Gln9 of histone H2B is selectively transamidated with the e-amino group of Lys5 or Lys12 of histone H4 in ... H4 of an adjacent nucleosome core. Incorporation of DCA into histones We next addressed the issue of whether transglutaminase is involved in the formation of...

Ngày tải lên: 31/03/2014, 07:20

... asympto- matic carriers of Hz-2V. The ability of this virus to persist in these asymptomatic carriers is a key feature of the biol- ogy of this virus. Since productive replication of Hz-2V results in ... or 2 × 10 8 TCID 50 units of Hz-2V. BioMed Central Page 1 of 7 (page number not for citation purposes) Virology Journal Open Access Research In vivo dose-response of insec...

Ngày tải lên: 20/06/2014, 04:20

... anti-BrdU antibody (1:50, DAKO), as de- scribed previously [4]. The labeling index was calcu- lated by expressing the number of BrdU-positive epithelial cells as a percentage of the total number of ... observed in group B (Fig 2B). However, the degree of mucosal infiltration of inflammatory cells (both of neutrophilic polymor- phonuclear and mononuclear cells) in group A...

Ngày tải lên: 08/08/2014, 16:23

... monolayers of Vero cells were infected at an MOI of 0.01 and the infected cells were harvested at 1, 24, 36 and 48 hpi. Viral titers were deter- mined by a standard plaque assay in Vero cells [39]. Competing ... implications of these data are discussed. Results Sequence analysis of the 5' upstream of hsc70B Transcription factor binding elements along the 5'- upstream...

Ngày tải lên: 20/06/2014, 01:20

... The very low density lipoprotein receptor gene (VLDLR), a member of the low density lipoprote in receptor (LDLR) gene family, plays a crucial role in the synthesis of yolk protein precursors in ... Genotyping of other indivi- duals of the second and third generations were carried out by PCR-RFLP. PCR for genotyping were performed in a volume of 15 μL consisting of 50-300 n...

Ngày tải lên: 14/08/2014, 13:21

... China 3 Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education and Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture, Huazhong ... expression analysis of the porcine CA3 gene in different pig breeds. Real-time PCR analysis of the CA3 gene in the skeletal muscle of Yorkshire and Meishan pigs at month-2. Rel...

Ngày tải lên: 14/08/2014, 13:22