Báo cáo sinh học: "Human cytokine-induced killer cells have enhanced in vitro cytolytic activity via non-viral interleukin-2 gene transfer" docx

... secreting lymphocytes functioning as immune enhancer cells. Therefore, our report is the first describing CIK cells to have enhanced in vitro cytolytic activity via non-viral interleukin-2 gene transfer ... by stimulating immune cells including T and natural killer cells. But adenoviral transfection may raise safety questions in human gene therapy. Therefore, w...

Ngày tải lên: 14/08/2014, 19:22

... had any effect on Ad5 binding to cells. To distinguish viruses bound to cells from viruses internalized into the cells, we performed a cell binding assay at 4°C since Ad internali- zation occurs ... in the cell lysates. We found that Ad5.PTD- tat exhibited a significant higher cell-binding activity in almost all of the cells we examined, including both high- CAR and low-CAR conta...

Ngày tải lên: 18/06/2014, 18:20

... Recording strategies and selection potential of feed intake measured using the X-ray method in rainbow trout, Genet. Sel. Evol. 38 (2006) 389–409. [22] Kause A., Tobin D., Houlihan D.F., Martin ... Biometrical Genetics, 31600 Jokioinen, Finland b Finnish Game and Fisheries Research Institute Jyväskylä, Survontie 9, 40500 Jyväskylä, Finland c Finnish Game and Fisheries Research Institute, .....

Ngày tải lên: 14/08/2014, 13:22

... the pharmacological induction of CAR using histone deacetylase inhibitor (iHDAC) compounds is a viable strategy to enhance adenoviral mediated gene delivery to cancer cells in vitro. The incorporation ... upregulation in a clinical setting using VPA. Discussion The success in the clinical translation of gene therapy strategies in the context of neoplastic disease depends on add...

Ngày tải lên: 14/08/2014, 19:22

... (ICC) SH-SY5Y cells and mixed spinal cord cell cultures were processed for β-Gal staining 3 days after transduction using an X-Gal staining kit (Invitrogen). In order to iden- tify neurons and glia in the ... distinct patterns of transduction in vitro. Finally, uptake and retrograde axonal transport have been evaluated both in vitro using compartmentalized cham- bers and in...

Ngày tải lên: 14/08/2014, 19:22

... observed in the same oocyte (fig 4). King WA, Bousquet D, Greve T, Goff AK (1986) Meiosis in bovine oocytes matured in vitro and in vivo. Acta Vet Scand 27, 267-279 King WA, ... (1971) Cytogenetic analysis of pig oocytes matured in vitro. J Exp Zool 176, 383-396 Madison VJ (1988) Factors influencing in vitro maturation and fertilizati...

Ngày tải lên: 14/08/2014, 20:20

... horse oocytes in vitro. Genet Sel Evol 22, 151-160 Krallinger HF (1927) Uber die Chromosomenzahl beim Rind sowie einige allgemeine Bemerkungen uber die Chromosomenforschung in der ... Commun 3, 51-56 King WA, Bousquet D, Greve T, Goff AK (1986) Meoisis in bovine oocytes matured in vitro and in in vivo. Acta Vet Scand 27, 267-279 King W...

Ngày tải lên: 14/08/2014, 20:20

... differentiation, blast cells have been resus- pended in EGM-2 complete media (Cambrex) and incu- bated in fibronectin coated plates (Becton Dickinson) for 5 days. To prove that fibronectin-adhering cells are ... System (Invitrogen) using the oligo(dT) method according to manufacturers' protocols. In sam- ples from single-colonies, cDNA was prepared using CellsDirect cDNA Synt...

Ngày tải lên: 18/06/2014, 15:20

... other equally important deter- minants of health. Investing in HRH, in addition to increasing health expenditure, expanding female educa- tion and raising national income will help countries, par- ticularly ... and other determinants in affecting health outcomes. An implication of our results is that investing and pouring in more money to increase the number of physicians and nurses...

Ngày tải lên: 18/06/2014, 17:20

... immunization including inadequate financing, poor vaccine quality, poor vaccination prac- tices, and weak health care systems [7] but one of the most common general barriers to improving immunization rates ... resource management in the health facilities. The doctor is appointed by the head of the policlinic, and the head of the policlinic is appointed by the Ministry of Property Management...

Ngày tải lên: 18/06/2014, 17:20