Báo cáo sinh học: "Attenuation of dengue virus infection by adeno-associated virus-mediated siRNA delivery" pdf

... Central Page 1 of 10 (page number not for citation purposes) Genetic Vaccines and Therapy Open Access Research Attenuation of dengue virus infection by adeno-associated virus- mediated siRNA delivery Weidong ... Corresponding author dengue virussiRNAgene expressionadeno-associated virus Abstract Background: The need for safe and effective treatment of dengue virus (...

Ngày tải lên: 14/08/2014, 19:22

... enhanced by viral infection (Fig. 5), thus failing to provide evidence for upregulation of RSV-F expression by another viral fac- tor. Chimeric ORF of RSV-F revealed strong dependency of protein ... occur during Pol II dependent expression of RNA virus proteins can be overcome by changing the codons of viral genes to those most frequently used by the genes of the host...

Ngày tải lên: 18/06/2014, 18:20

... effect of site of initial infection and hetero- typic immunity. Infect Immun 1980, 29(2):654-662. 31. Yap KL, Ada GL: The recovery of mice from influenza A virus infection: adoptive transfer of ... recovery from pneumo- nia caused by an influenza virus infection [1,2]. Proof was provided using adoptive transfer of CD8 + T cells and clones [3,4,1], in vivo depletion of...

Ngày tải lên: 12/08/2014, 15:20

... Vaccinia virus; IV: Immature virus; IMV: Intracellular mature virus; IEV: Intracellular enveloped virus; WR: VV Western Reserve strain; SD: Site-directed mutagenesis; MOI: Multiplicity of infection; ... cleavages are driven by the VV I7L cysteine proteinase, it was likely that A12L might be another substrate of I7L. By taking advantage of a temper- ature-sensitive mutant virus...

Ngày tải lên: 18/06/2014, 18:20

... diagnosis of dengue virus infection is primarily achieved by the isolation of virus, detection of IgM/IgG antibodies by serodiagnosis and/or molecular detection by the demonstration of viral RNA by ... diagnosis of dengue virus infection. Acta Virologica 2000, 45:299-304. 12. Yamada K, Takasaki T, Nawa M, Kurane I: Virus isolation as one of the diagnostic me...

Ngày tải lên: 20/06/2014, 01:20

... Genetic analysis of Dengue 3 virus subtype III 5’ and 3’ non-coding regions. Virus Res 2008, 135:320-325. 11. Weaver SC, Vasilakis N: Molecular evolution of dengue viruses: contributions of phylogenetics ... regions of the world. This suggests a lack of correlation among DENV genotype III substitution rate and ecological pattern of virus spread. Background Dengue viru...

Ngày tải lên: 12/08/2014, 02:20

... formation of O 2 – through the partial reduc- tion of oxygen by one electron. Subsequent reduction by one or two electrons can yield H 2 O 2 , and HO, respect- ively [46]. However, the main source of ... else- where [11,12]. The lack of mitochondrial contamina- tion with cytosolic NOS was assessed by the absence of any detectable NOS activity and nitrite levels in the supernat...

Ngày tải lên: 19/02/2014, 00:20

... improvement of con- tractile function. The underlying cause of weakened contractile perfor- mance of the end-stage heart failing myocardium is often indep endent of the originating cause of heart ... beyond the scope of this end-point driven study. Discussion Cardiac contractile dysfunction is one of the leading causes of death in DMD. Clinical treatment of this debilitating...

Ngày tải lên: 18/06/2014, 19:20

... generation of recombinant vesicu- lar stomatitis virus (VSV) [6,7] and respiratory syncytial virus (RSV) [8] by reverse genetics. These systems are based on cytoplasmic transcription of viral cDNA by ... expression of heterologous genes not adapted to the nuclear environment. Using the open reading frames of the G protein of VSV and the F protein of RSV as representatives...

Ngày tải lên: 20/06/2014, 01:20

... characterized by the presence of target binding sites for transcription factors (TFs). Knowledge of the identity of a mediating TF can give important insights into the func- tion of a gene via inference of ... methods for the detection of regulatory regions. One approach of growing popularity is based on the preferential conservation of functional sequences over the course...

Ngày tải lên: 06/08/2014, 18:20