Báo cáo sinh học: "Novel non-viral method for transfection of primary leukemia cells and cell lines" docx

... a density of 1 × 10 6 cells per ml. Nucleofection of cells Primary AML cells, K562 and HL60 cells were transfected by nucleofection with the optimised conditions by using the Nucleofector system ... The transfection data of the pri- mary AML cells of three patients and flow cytometric analysis are shown in figure 2a and 2b. Figures 1b (K562 cells) and 1c (HL60 c...

Ngày tải lên: 14/08/2014, 19:22

... implementation of a stand alone H.264 q-pel MC on FPGA has shown speeds between 95.9 fps for HD1080p frames, 229fps for HD 720p and between 2502 and 12623 fps for CIF and QCIF formats. Keywords: ... has been used. A stand alone H.264 q-pel MC block has achieved speeds between 95.9 fps for HD1080p frames, 229fps for HD 720p and between 2502 and 1262 fps for CIF and QC...

Ngày tải lên: 18/06/2014, 22:20

... lanes 1 and 2 versus 3 and 4). Random hexamers (Rndm) were more efficient than B4R1, a primer specific to the 3'-UTR (lanes 3 and 4 versus 5 and 6, or lanes 7 and 8 versus 9 and 10). For amplicon ... time of 2.5 min was used for ampli- cons over 2 kb (amplicons 1, 2 and 3), and extension time of 2 min was used for amplicons less than 2 kb (ampli- cons 1x, 1y, 4x...

Ngày tải lên: 19/06/2014, 08:20

... copies of a parser, each of which trained on a different sample from the training data, to predict the quality of a parse. The algorithm does not assume uniformity of training and test data, and ... lines) and Charniak (bottom two lines) model, in the two scenarios (in-domain and adaptation), vs. the min- imum length (ML lines 1 and 3) and confidence (CB, lines 2 and 4) b...

Ngày tải lên: 20/02/2014, 12:20

... words with a cutoff of N=10. The resulting n-gram set had a maximum recall of 2,610 phrasal terms from the WordNet gold standard, and found the best figure of merit for any of the association ... Klavans and E. Tzoukermann (1997). Expansion of multiword terms for indexing and retrieval using morphology and syntax. Proceedings of the 35th Annual Meeting of the A...

Ngày tải lên: 08/03/2014, 04:22

... different provinces of Zambia, with a view to assess the dimension of shortages and of imbalances in the distribution of health workers by province and by level of care. Population and methods We ... referral of patients, human resources and facilities. Right-sizing of the public sector facilities and the gradual increase of for- profit” and “not -for- profi...

Ngày tải lên: 18/06/2014, 17:20

... with the CRI-MAP 2.4 software [19], showed an average marker interval of 17.86 cM [18] and an information content (IC) for QTL detection of about 0.6 [17]. The use of this map for the parasite resistance genome ... http://www.gsejournal.org/content/41/1/46 Page 10 of 10 (page number not for citation purposes) 28. Weller JI, Kashi Y, Soller M: Power of daughter and granddau...

Ngày tải lên: 14/08/2014, 13:21

... method of Rendel and Robertson (1950), our procedure is based on a comparison of successive generations and is, thus, of a simpler formulation than iterative methods. METHODS Description ... demonstration of the formula of Rendel and Robertson (1950); the second is one in which individuals of the same group have the same age, similar to...

Ngày tải lên: 14/08/2014, 20:20

... present in the cells of strain TOY3, consistent with the idea that the TOY1 and TOY3 cells are useful for production of UDP-GlcA and UDP-Xyl, respectively. In order to quantify the levels of UDP-sugars ... was observed in TOY1 cells (Fig. 3B and Table 1). More- over, UDP-GlcA accumulated in TOY1 cells at a level of 5.71 lmolÆg )1 (dry weight) and was not detected in...

Ngày tải lên: 07/03/2014, 12:20

... BRL cells were obtained from RIKEN Cell Bank (Tsuku ba, Japan). Rat pituitary-derived GC cells were obtained from M. Karin (University of California, San Diego, CA, USA). The Y3 and BRL cells ... JRH Bioscience s, US A) and the GC cells were Fig. 1. Expression of B29/Ig-b mRNA (A) and DNase I hypersensitivity by SkM sodium channel and B29/Ig-b gene chromatins of Y3 ce...

Ngày tải lên: 24/03/2014, 03:21