Báo cáo y học: "Detection of weakly conserved ancestral mammalian regulatory sequences by primate comparisons" doc

... exons Conserved sequences overlapping regulatory elements 20 (16%) 11 (9%) Conserved sequences of unknown function Evolutionary conservation of six primate -conserved sequences in anthropoid primates, ... gene regulatory activity in a variety of in vitro and in vivo assays. These three regulatory sequences are so weakly conserved in distant, non -primate mamma...

Ngày tải lên: 14/08/2014, 17:22

... Laemmli [32]. Assay of cell-free HA synthesis Cell-free HA synthesis was performed by a modification of the method reported by Nakamura et al. [18]. Analysis of the transfer of UDP-[U- 14 C] GlcUA ... pathogenicity by allowing them to escape from the immune systems of their hosts. The HA synthesized by Streptococci is not chemically or structurally distinguishable from that s...

Ngày tải lên: 08/03/2014, 09:20

... radiography, and showed a mark- edly higher sensitivity of MRI than radiography when CT was considered the reference method. Secondly, when measuring erosion volumes by CT and MRI, a very high intramodality ... potentially a major source of error. Especially, the peripheral border of erosions can be difficult to define as sig- nal intensities of erosions and adjacent soft-tissues of...

Ngày tải lên: 09/08/2014, 10:23

... Taq polymerase buffer and Taq-polymerase in thermocycler (Applied Biosystems) with cycling conditions of 94°C, 10 min followed by 35 cycles of 94°C, 1 min, 55°C, 1 min, 72°C, 1 min. Presence of the ... More reliable diag- nosis of infection with human immunodeficiency virus type 1 (HIV-1) by detection of antibody IgGs to pol and gag proteins of HIV-1 and p24 antigen of HIV-1...

Ngày tải lên: 10/08/2014, 05:21

... University in Cairo, New Cairo, Egypt. 3 Department of Biochemistry, University of Saskatchewan, Canada. 4 Faculty of Computers & Information, Cairo University, Ahmed Zowail Street, Cairo, Egypt. 5 Biology Department, ... in the performance with NM and MK. Analysis of results by NM, MK, IE, RS, and SZ. Writing by ME, MK, NM, and IE. Revision by ME, RS, IE and SZ. Scripting...

Ngày tải lên: 11/08/2014, 21:22

... every day from 1 st day of hospitalisation. @1 - Day of admission. @2 - Day of discharge/death. Cecilia et al. Virology Journal 2011, 8:46 http://www.virologyj.com/content/8/1/46 Page 2 of 4 India/2009) ... suffered primary or secondary infections. HI antibody titre of >1:2560 in the acute phase of infection is considered confirmatory of secondary infection [8]. Case 1 had ve...

Ngày tải lên: 11/08/2014, 21:22

... TGEV-LAMP was analyzed by agarose gel electrophoresis and fluorescence by adding 1 × SYBR greenI in the LAMP reaction. LAMP evaluation The specificity of TGEV-LAMP w as examined by the use of RNA (or ... The high specificity of LAMP is most probably attributable to recognition of the target sequence by six independent sequences in the initial stage and by four independent...

Ngày tải lên: 12/08/2014, 01:21

... waters. Typically we utilize monolayers of 3 cell lines; buffalo green monkey kidney (BGM), human colonic carcinoma (CAC O-2) and African rhesus monkey kidney (MA104) with the intent of provid- ing ... on day 6 at -80°C and stored until the RNA extraction proce dure was performed. Because of the highly lytic nature of poliovirus type 1 strain LSc-1 and the duration of the experiment...

Ngày tải lên: 12/08/2014, 01:22

... synthesized by Saituo * Correspondence: cuishangjin@yahoo.cn † Contributed equally 3 Division of Swine Infectious Disease, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary ... parvovirus, subfamily Parvoviri- nae,familyParvoviridae. It is one of the major etiologi- cal agents of reproductive failure in pigs. Reproductive failure caused by PPV is characterized b...

Ngày tải lên: 12/08/2014, 02:20

... AF119661, DQ181799, AY702040 , U88535, DQ285561, AY708047, AF298807, AY762084, AY776330, AY762085, AY618988, AF100466, AY858044, AY858047 , AY744685, AF013407, AF013383, AY632541, NC009029, DX03700, ... used in the phyloge- netic study (Accession Number): GenBank: AF202541 , DQ118127, AY278441, AY278442, AF260968, D00246, AY274505, DQ256376 , AY688948, DQ116961, DQ318020, AY277251, AY76526...

Ngày tải lên: 12/08/2014, 04:21