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Báo cáo y học: "Detection of weakly conserved ancestral mammalian regulatory sequences by primate comparisons" doc

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Báo cáo y học: "Detection of weakly conserved ancestral mammalian regulatory sequences by primate comparisons" doc

... exons Conserved sequences overlapping regulatory elements20 (16%)11 (9%) Conserved sequences of unknown function Evolutionary conservation of six primate -conserved sequences in anthropoid primates, ... gene regulatory activity in a variety of in vitro and invivo assays. These three regulatory sequences are so weakly conserved in distant, non -primate mammals that none of thethree independent ... the feasibility of genome-wide discovery of primate -conserved regulatory elements.Sequence divergence of the identified regulatory elementsbetween human and distant mammals may reflect functionalchanges...
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Báo cáo Y học: Inhibition of hyaluronan synthesis in Streptococcus equi FM100 by 4-methylumbelliferone doc

Báo cáo Y học: Inhibition of hyaluronan synthesis in Streptococcus equi FM100 by 4-methylumbelliferone doc

... Laemmli [32].Assay of cell-free HA synthesisCell-free HA synthesis was performed by a modification of the method reported by Nakamura et al. [18]. Analysis of the transfer of UDP-[U-14C] GlcUA ... pathogenicity by allowingthem to escape from the immune systems of their hosts.The HA synthesized by Streptococci is not chemicallyor structurally distinguishable from that synthesized in mammalian ... treatment may causea decrease in hyaluronan synthase activity by altering thelipid environment of membranes, especially the distribution of different cardiolipin species, surrounding hyaluronansynthase.Keywords:...
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Báo cáo y học: "Detection of bone erosions in rheumatoid arthritis wrist joints with magnetic resonance imaging, computed tomography and radiography" ppt

... radiography, and showed a mark-edly higher sensitivity of MRI than radiography when CT wasconsidered the reference method. Secondly, when measuringerosion volumes by CT and MRI, a very high intramodality ... potentially a major source of error. Especially, theperipheral border of erosions can be difficult to define as sig-nal intensities of erosions and adjacent soft-tissues often arevery similar ... http://arthritis-research.com/content/10/1/R25Page 5 of 8(page number not for citation purposes)The even higher agreement (87% vs 77%) between CT andMRI in a study of nine RA wrist joints by Perry and colleagues[12] may partly be explained...
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Báo cáo y học: " Detection of HIV-1 RNA/DNA and CD4 mRNA in feces and urine from chronic HIV-1 infected subjects with and without anti-retroviral therapy" pptx

... Taqpolymerase buffer and Taq-polymerase in thermocycler(Applied Biosystems) with cycling conditions of 94°C, 10min followed by 35 cycles of 94°C, 1 min, 55°C, 1 min,72°C, 1 min. Presence of the ... More reliable diag-nosis of infection with human immunodeficiency virus type 1(HIV-1) by detection of antibody IgGs to pol and gag proteins of HIV-1 and p24 antigen of HIV-1 in urine, saliva, ... 67% of subjects' fecal sam-ples by RT-nested PCR. Since the subjects involved in theirstudy were in early stages of HIV-1 infection, when therapid replication of HIV-1 and destruction of...
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Báo cáo y học: " Identification of novel conserved functional motifs across most Influenza A viral strains" pps

... University in Cairo, New Cairo, Egypt.3Department of Biochemistry, University of Saskatchewan, Canada.4Faculty of Computers &Information, Cairo University, Ahmed Zowail Street, Cairo, Egypt.5BiologyDepartment, ... in the performancewith NM and MK. Analysis of results by NM, MK, IE, RS, and SZ. Writing by ME, MK, NM, and IE. Revision by ME, RS, IE and SZ. Scripting by OA, MK andNM. All authors read and ... Entropy calculationMining for conserved sequences among the aligned sequences was performed by determining the entropy of regions with at least 21 nucleotides in length with amaximum of 2 mismatches....
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Báo cáo y học: " Detection of dengue-4 virus in pune, western india after an absence of 30 years - its association with two severe cases" docx

... every day from 1stday of hospitalisation.@1- Day of admission.@2- Day of discharge/death.Cecilia et al. Virology Journal 2011, 8:46http://www.virologyj.com/content/8/1/46Page 2 of 4India/2009) ... suffered primary orsecondary infections. HI antibody titre of >1:2560 in theacute phase of infection is considered confirmatory of secondary infection [8]. Case 1 had very low levels of HIantibodies ... inical eva-luation and physical examinations. The case historyforms of the patients were filled from the time of admis-sion. Neither of the patients had any recorded history of dengue infection...
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Báo cáo y học: " Detection of swine transmissible gastroenteritis coronavirus using loop-mediated isothermal amplification" pptx

... TGEV-LAMP was analyzed by agarosegel electrophoresis and fluorescence by adding 1 ×SYBR greenI in the LAMP reaction.LAMP evaluationThe specificity of TGEV-LAMP w as examined by theuse of RNA (or ... Thehigh specificity of LAMP is most probably attributableto recognition of the target sequence by six independent sequences in the initial stage and by four independent sequences during the ... shown in Table 1.Detection of TGEV by LAMPTGEV deprived from the cell culture was first qualita-tively analyzed by LAMP. Amplification products wereanalyzed by agarose gel electrophoresis....
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Báo cáo y học: "Detection of poliovirus by ICC/qPCR in concentrated water samples has greater sensitivity and is less costly using BGM cells in suspension as compared to monolayers" pot

... waters. Typically we utilize monolayers of 3 cell lines; buffalo green monkey kidney(BGM), human colonic carcinoma (CAC O-2) and African rhesus monkey kidney (MA104) with the intent of provid-ing ... on day 6at -80°C and stored until the RNA extraction proce durewas performed. Because of the highly lytic nature of poliovirus type 1 strain LSc-1 and the duration of theexperiment only 1 freeze/tha ... to each of the three monolayers and three suspensions. Themonolayers were incubated at 37°C for 80 min to allowfor adsorption of the PV1. They were subsequentlyreturned to the safety hood for...
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Báo cáo y học: " Detection of porcine parvovirus using a taqman-based real-time pcr with primers and probe designed for the NS1 gene" doc

... synthesized by Saituo* Correspondence: cuishangjin@yahoo.cn† Contributed equally3Division of Swine Infectious Disease, State Key Laboratory of VeterinaryBiotechnology, Harbin Veterinary ... parvovirus, subfamily Parvoviri-nae,familyParvoviridae. It is one of the major etiologi-cal agents of reproductive failure in pigs. Reproductivefailure caused by PPV is characterized by embryonicand ... TheTaqManreal-timePCRassaywillbeusefulforstudying the molecular epidemiology of PPV infectionsin swine populations. The assay will also be very usefulfor early diagnosis of PPV and therefore for manage-ment of...
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Báo cáo y học: "Detection of novel insect flavivirus sequences integrated in Aedes albopictus (Diptera: Culicidae) in Northern Italy" potx

... AF119661, DQ181799, AY702040, U88535, DQ285561, AY708047, AF298807, AY762084, AY776330, AY762085, AY618988, AF100466, AY858044, AY858047, AY744685, AF013407, AF013383, AY632541, NC009029, DX03700, ... used in the phyloge-netic study (Accession Number): GenBank: AF202541, DQ118127, AY278441, AY278442, AF260968, D00246, AY274505, DQ256376, AY688948, DQ116961, DQ318020, AY277251, AY765264, AF013384, ... with ABI Prism BigDye TerminatorCycle sequencer v3.1 ready reaction (Applied Biosystems)and analyzed with the ABI PRISM 377 DNA Analyzer(Applied Biosystems). Sequence analysis was carried outwith...
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