Báo cáo y học: "Analysis of genetic systems using experimental evolution and whole-genome sequencing" docx

... Hegreness and Kishony http://genomebiology.com/2007/8/1/210 Genome Biology 2007, 8:201 Genome Biology 2007, 8:201 Minireview Analysis of genetic systems using experimental evolution and whole-genome ... Hegreness* † and Roy Kishony* ‡ Addresses: *Department of Systems Biology, Harvard Medical School, Longwood Avenue, Boston, MA 02115, USA, † Department of Organismic...

Ngày tải lên: 14/08/2014, 17:22

... Genomewide analysis of mRNA processing in yeast using splicing-specific microarrays. Science 2002, 296:907-910. 22. Yeakley JM, Fan JB, Doucet D, Luo L, Wickham E, Ye Z, Chee MS, Fu XD: Profiling alternative ... sample of 153 exon-skipping events predicted by the microarray analysis, 73 were successfully validated by RT- PCR and sequencing (a 48% validation rate). This initial study h...

Ngày tải lên: 09/08/2014, 20:20

... pulmo- nary fibrosis (IPF) is characterized by a predominance of gene expression for Th2-type regulatory cytokines. This pattern is associated with high levels of IL-4 and IL-5 and a paucity of IFN-γ ... identical conditions and as previously described [26,27]. Data Analysis The genotype frequencies, allele carriage frequency and allelic frequency were determined by direct count...

Ngày tải lên: 13/08/2014, 13:20

... therapy, focusing on a potential genetic clue to medication response. Key words: asthma, genetic polymorphisms O ur understanding of the pathophysiology and treat- ment of asthma is quickly advancing. ... majority of studies indicate that the Gly16 SNP appears to carry the greatest potential clinical impact. The wild genetic makeup of the sixteenth amino acid position of the...

Ngày tải lên: 08/08/2014, 21:20

... Doyle A: Patterns of psychiatric comorbid- ity, cognition, and psychosocial functioning in adults with attention deficit hyperactivity disorder. Am J Psychiatry 1993, 150:1792-1798. 23. Ratey ... Consumer and Specialty Pharmaceuticals. Authors' contributions JK and ML conceptualized and designed the study. ML had primary responsibility for analysis of and interpreta- tion...

Ngày tải lên: 08/08/2014, 21:20

... University. Genetic analyses DNA was extracted by the salting-out method described by Miller and colleagues [18]. Analysis of genetic polymor- phism was predominantly performed by polymerase chain reaction ... previously described [20]. Analysis of Fc γ RIIIa gene polymorphism The analysis of the F/V polymorphism was performed essentially as previously described [21]. MBL gene pol...

Ngày tải lên: 09/08/2014, 01:24

... analyses of the frequency of occurrence and mutations of the highly mutable motifs RGYW and WRCY revealed that mutations in nonproductive V L rearrange- ments of B cells from the parotid gland ... nonproductive and productive repertoires of these donors exhibited a comparable usage of Vκ and Jκ as well as Vλ and Jλ gene elements [10,11]. Determination of Taq polymerase...

Ngày tải lên: 09/08/2014, 03:24

... cytotoxic granule and cytokine release), antigen presen- tation and antibody-dependent cellular cytotoxicity [6]. Con- versely, FcγRIIb contains an inhibitory motif in the cytoplasmic tail and ... the collection of clinical data and the recruitment of patients into the study. DS, JR and VK undertook some of the genotyping assays on DNA prepared in the laboratory of EAJ and...

Ngày tải lên: 09/08/2014, 07:20

... followed by 40 cycles of 95°C for 1 minute and 60°C for 15 seconds, as recommended by the manufacturer (Applied Biosystems). Real-time data were ana- lysed by using the Sequence Detection Systems software, version ... 1 Standard curves generated for each assay by ten fold serial dilutions of template,withreal-time data analysed by using the Sequence Detection Systems software,...

Ngày tải lên: 09/08/2014, 08:22

... cultural examination of common bacteria and fungi, and of direct acid-fast bacilli smears (Kinyoun method) and cultures for mycobacteria, and for the microscopic examination of Pneumocystis jiroveci ... of pro-inflammatory cytokines, the influx of inflammatory cells, and fibrinogenesis, whereas the downregulation of GSTP, Cu–Zn SOD and cystatin SN could favor vascular...

Ngày tải lên: 09/08/2014, 08:22