Báo cáo y học: "The many routes to regulating mRNA translation" pot

... thereby facilitating poly(A) addition catalyzed by another complex member, Gld-2, a noncanonical poly(A) polymerase. Using polyadenylation state array (PASTA) analysis to separate and identify mRNA ... an mRNA can cycle between repressed and translationally active states, and that this cycling may involve a spatial segregation within the cell cytoplasm, was supported by Roy Parker (Univ...

Ngày tải lên: 14/08/2014, 17:22

... separately for microarray analysis. Microarray and cluster analysis All experiments for microarray analysis were performed inde- pendently, in triplicate, to facilitate statistical analysis. Total RNA ... 10, and 12 hours relative to pupariation, labeled, and hybridized to Affymetrix Drosophila Genome Arrays. The sensitivity and accuracy of the array data were determined by comparing th...

Ngày tải lên: 14/08/2014, 15:20

... likely to be easy to both genotype and sequence. The sensitivity of SNP and indel detection in repetitive or copy-number-variable regions will be lower. Finally, a non-trivial proportion of any ... missed is likely to be substantially higher, however, as the set of ‘true’ calls used to derive the SNP sensitivity estimates (from the Illumina 610K genotyping array) is biased towar...

Ngày tải lên: 09/08/2014, 20:20

... self-certainty factor correlated signifi- cantly with the PANSS total score and negative symptom and positive symptom factors, while the composite index correlated negatively with cognitive factors, ... contributions YCK wrote the draft of this manuscript. YCK and YPL conceptualised and designed the study. YCK collected and analysed the data. YPL supervised the study. YCK analysed the data fu...

Ngày tải lên: 11/08/2014, 16:22

... we are trying to achieve two things with the same system? It has got to the point now where you want a system to enroll people into studies, and at the other end you want a system where you have ... therapy. So I think if the purpose of this is to try and speak a language that everybody understands, to classify patients so that you can group them together in clinically relevant ways...

Ngày tải lên: 12/08/2014, 19:22

Ngày tải lên: 12/08/2014, 20:20

... positive end-expiratory pressures in patients with the acute respiratory distress syndrome. N Engl J Med 2004, 351:327-336. 4. Meade MO, Cook DJ, Guyatt, GH, Slutsky AS, Arabi YM, Cooper DJ, Davies, ... strategy may be not be adequate for all patients. For a given level of PEEP, transpulmonary pressures may vary widely from patient to patient. If the clinician could measure transpulmonar...

Ngày tải lên: 13/08/2014, 21:21

... display, yeast two-hybrid analysis, or any experiment that requires specific antibodies, are difficult or impossible to apply to transmembrane regions of membrane proteins. This makes it hard to ... problem and method. Although it is not yet possible to apply automated methods to provide any desired function, computational design is well suited to identifying combinations of amino...

Ngày tải lên: 14/08/2014, 07:21

... zinc by other metals) may contribute to male infertility. Exposure of males to certain alkylating agents has also been reported to have an adverse impact on repro- duction by modifying cysteine ... delivery to hepatoma cells using galactosylated liposome-polycation- DNA complexes (LPD). J Drug Target 2005, 13:121-128. 81. EBI tools: ClustalW [http://www.ebi.ac.uk/clustalw] http://geno...

Ngày tải lên: 14/08/2014, 08:20

... responses by providing costimulatory or coinhibitory signals upon binding their receptor. Costimulatory signals are defined as those that act in conjunction with antigen-receptor signals, leading to ... ligands for the PD-1 receptor, is differentially regulated by cytokines: interleukins IL-4 and IL-13 upregulate PD-L2 [29,30], whereas inflammatory cytokines, primarily interferon-␥, modulat...

Ngày tải lên: 14/08/2014, 14:21