Báo cáo y học: "Global analysis of mRNA stability in the archaeon Sulfolobus" pps

... original work is properly cited. Sulfolobus mRNA stability& lt;p>A microarray-based analysis of mRNA half-lives in two species of Sulfolobus, an hyperthermophilic archaeon, shows that their mRNA ... eukaryotes and bacteria. In contrast, relatively little is known about RNA stability in the third domain of life, Archaea. Here, we present a microarray-based analysi...

Ngày tải lên: 14/08/2014, 17:22

... 5' end, thereby fluorescently labeling the sense strand of the PCR product. The PCR product was denatured and then hybridized to the microarray. As in microarray analysis, the labeled sample ... Quantification of microarray data using three synthetic RNAs: syn1, syn2 and syn3. Each data point is the average of two independent labelling/hybridization reactions. Pro...

Ngày tải lên: 14/08/2014, 14:21

... protein degradation, cell divi- sion, energy metabolism and RNA binding proteins. The majority of transcripts for RNA binding proteins are depos- ited maternally into the early embryo, highlighting ... Nucleolus Chromatin binding Chromosome Helicase RNA binding Cytosol Nuclease activity Ubiquitin conj. enzy Protein biosynthesis Mitochondrion Methyltransferase Spindle GPCR Vitamin bindi...

Ngày tải lên: 14/08/2014, 07:22

... upon activation of the insulin and wingless pathways in S2 cells Binding of insulin-like peptides to the insulin receptor in Drosophila cells leads to the activation of dPI3 kinase, which http://genomebiology.com/2009/10/1/R11 ... splicing can play a mechanistic role in mediating the effects of insulin and wingless on alter- native splicing. Numerous changes in altern...

Ngày tải lên: 14/08/2014, 21:20

... clus- tering of these genes was then performed to identify gene expression patterns among the study samples. As in the JDM study, a striking increase in the expression of a group of genes previously ... the interferon (IFN) pathway in systemic lupus erythematosus (SLE) [3–6]. Analysis of microarray data A statistical analysis of appropriately normalized micro- arra...

Ngày tải lên: 09/08/2014, 01:23

... against this pro-inflammatory cytokine. Interestingly in our PLCH patients this mechanism was down-regulated. The results of our proteome analysis of PLCH BAL suggested the involvement of some ... Other proteins like purine nucleoside phosphorylase, pyruvate kinase isozymes, fibrinogen gamma chain, alpha 1B glycoprotein and actin cytoplasmic 1 were identified. BAL proteome a...

Ngày tải lên: 10/08/2014, 09:22

... represent the inclusion lines (dot lines) and the lower and left lines including the extensions are the exclusion lines. Any profile of myelinated nerve fiber section hitting the exclusion lines is ... this amino acid occurs in only a few other pro- teins like elastin [30,31]. Therefore, hydroxyproline has been used for many years as a means of determining the amount of...

Ngày tải lên: 10/08/2014, 10:20

... (70.7%) of the boys and 39 (47.0%) of the girls were diagnosed with the com- bined subtype. The predominantly inattentive subtype was diagnosed in 86 (25.4%) of the boys and 38 (45.8%) of the girls. ... obtained by using other instruments that distinguish between various times of the day, such as the Daily Parent Rating of Evening and Morning Behavior (DPREMB) sc...

Ngày tải lên: 13/08/2014, 18:21

... determine better whether the novel transcripts may be functional, we examined their ability to encode pro- tein. The sequences of RACE products were analyzed with respect to whether they contain ... gain a better understanding of why the SYN3 and TIMP3 genes were not detected by microarray analysis, we examined their expression level by real-time quantitative PCR. As shown in Fig...

Ngày tải lên: 14/08/2014, 08:20

... down-regulation in the autopsy samples. Interestingly, we also see up-regulation of genes involved in the ubiquitin cycle, protein ubiquitination, and ubiquitin-dependent protein catabolism. This ... significantly in the two brain regions examined (see Material and methods). Numbers in brackets indicate the percentage of significant probe sets compared to the total nu...

Ngày tải lên: 14/08/2014, 16:20