Báo cáo y học: "Genomic analysis reveals that Pseudomonas aeruginosa virulence is combinatorial" docx

... directly with degree of virulence, suggesting that these genes do not function autonomously to affect virulence. Our genomic analysis of PA14 virulence has demonstrated that pathogenicity in this ... microarray experiments; D Park for assistance with microar- ray data analysis; and E Mylonakis for discussions regarding the evolution of fungal pathogens. We especially acknowledge S...

Ngày tải lên: 14/08/2014, 17:22

... after combined analysis applying Bonferroni P value correction. Additional data file 3 is a table listing Rhizosphere differentially expressed genes after com- bined analysis applying Benjamini ... image analysis software (Axon Instruments, Inc.). Spots with anom- alies were discarded and excluded from further analysis. Spot signal intensities were normalized by applying the Lowess i...

Ngày tải lên: 14/08/2014, 08:20

... evolutionary history and Table 3 SNP genotyping success categorized by polymorphic quality score Polymorphic quality score 2 3 4 5 Number of genotyped loci 5 15 28 11 Number of polymorphic loci ... as out- liers: a nonsynonymous polymorphism in csrp1 (F ST = 0.893), a synonymous polymorphism in β -catenin (Aln101106_1089; F ST = 0.904), and an intronic polymorphism in ptc2 (Aln100281_1741...

Ngày tải lên: 14/08/2014, 20:22

... previously reported that this dose of V 2 O 5 causes minimal cytotoxicity (<10% by lactate dehydrogenase assay) and yet induces H 2 O 2 pro- duction, activates intracellular signaling pathways ... It is possible that STAT1-dependent induction of CXCL9 and CXCL10 could be a mechanism for lymphocyte accumu- lation around airways and blood vessels following lung injury by V 2 O 5 . Po...

Ngày tải lên: 12/08/2014, 15:20

... man- ufacturers claim that the purity of the isolated cell popu- lation is high (i.e. > 95%), their recommended flow cytometry analysis to assess cell purity only makes use of an antibody against CD4, ... respectively. Signals were revealed using the ECL™ Western blotting detection rea- gent (Amersham, Piscataway, NJ). Densitometry analysis was performed using the freely available...

Ngày tải lên: 13/08/2014, 05:21

... datasets. Essentiality analysis for yeast and human genes confirms these findings. Motif analysis indicates conservation in the corresponding regulatory mechanisms. Gene Ontology analysis and analysis of the ... cycle characteristics when compared with the original set of cycling genes for each Analysis of cycling genes using complementary high throughput datasetsFigure 2 (see follo...

Ngày tải lên: 14/08/2014, 08:20

... regulation Glycan biosynthesis and metabolism Enzyme activity Signal transduction Amino acid metabolism Secondary metabolism Proteolysis and peptidolysis Lipid metabolism Carbohydrate metabolism Transport Number ... contributions YYO, TM and RAD designed the study; YYO, ND and SC car- ried out the experiments; YYO and HP performed data processing and statistical analysis; DB and SM created t...

Ngày tải lên: 14/08/2014, 08:21

... analysisFigure 1 Significantly differentially regulated pathways identified by GSEA analysis. The histogram represents the frequency distribution of maximum ES scores obtained with GSEA analysis ... associated pathway are differentially regulated to detect over-representation. A third is that the associated pathways primarily involve tissues that were not arrayed. That is, becaus...

Ngày tải lên: 14/08/2014, 14:21

... low-resolution cytological analysis. A comparison with studies using S. cerevisiae Hsf [27,28] sug- gest that a set of conserved genes are regulated by Hsf in both organisms. Overall, this study presents ... 'binding affinity' or that simple binding site prediction is not a reliable way of identifying genuine HSEs. Comparative analysis Two genome-wide studies in the budding...

Ngày tải lên: 14/08/2014, 14:21

... is most differentially expressed is missing sequence information no more frequently than expected by chance (χ 2 = 1.177, p = 0.2779). We repeated this analysis for the top 500 statistically significant ... coverage, intensity = 6.882; genes without coverage, intensity = 6.865). This analysis, however, may be con- founded by the fact that most genes are not statistically signif- ica...

Ngày tải lên: 14/08/2014, 20:22