Báo cáo y học: "Functional genomics of the yeast DNA-damage response" ppsx

... significantly by examining the genetic and physical context of the interactions. In other words, they attempt to place the transcriptional response to DNA damage in yeast within the context of the cell ... phenotype than disrup- tion of either gene alone) have been very successful at mapping functional pathways within yeast cells [20-22]. The diploid-based synthetic leth...

Ngày tải lên: 14/08/2014, 17:22

... the C-terminus of the receptor which is located on the inside of the cell, we then determined if signal transduction by the protein was affected by this modification. One of the consequences of ... version of CCR5 was present on the surface of transfected cells, and had the expected molecular mass. To test whether the presence of the myc-his tag at the C-ter...

Ngày tải lên: 08/03/2014, 09:20

... paper the complete resolution of the protein components of phycobilisomes from the cyanobacterium Synechocystis 6803, together with the determination of their molecular mass, has s uccessfully been ... a in the thylakoid membrane [5]. Phycocyanin is the major constituent of the phycobilisomes, while allophycoc yanin holds the bridging pigments between phycobilisomes an...

Ngày tải lên: 08/03/2014, 16:20

... immediately upstream of the TATA box in the p-126 minimal promoter construct completely abolished the stimulation of luciferase activity by CDCA in HepG2 cells (Fig. 6). Interestingly CDCA reduced the activity of ... activity of the mutant m-126 Luc even below that of the wild-type p-126 Luc. Analysis of the binding affinity of the putative FXRE site for the orp...

Ngày tải lên: 17/03/2014, 23:20

... general conservation of these other aspects of the glycome, dif- ferences suggest that there may be equally dramatic var- iations as observed for N-glycosylation. Cytoplasmic glycome Whereas glycosylation ... predominantly in the secretory compartments, formation of the precursors for these pathways generally originates in the cytoplasm, and the cytoplasm is also a s ite for c...

Ngày tải lên: 09/08/2014, 22:23

... amino group of side chain of lysine. The OD1 is one of the oxygen atoms present in the carboxyl group of the side chain within the aspartic acid. Discussion Thecleavagespecificityofalphavira l capsid ... analyze the influence of the C-terminal aa sequence for the activity of the ChikV capsid protease. As expected, mutating tryptophan in the 261 position to alan...

Ngày tải lên: 12/08/2014, 02:20

... early cortical patterning by signaling in the direction of the arrows (reviewed in [12]). (d) Sansom et al. [5] interrogated the genetic identity of the neocortex by performing a microarray study ... illuminate the underlying genetic control of the protomap model by using high-density oligonucleotide arrays to elucidate the transcriptional profile of discrete neocortical...

Ngày tải lên: 14/08/2014, 16:20

... coughs. We hypothesized that the expiratory period during cough is composed of two subphases each of which is regulated separately. Furthermore, we speculated that the duration of the expiratory interval ... ms). The ampli- tude of the ABD muscle EMG, amplitude of the PS muscle EMG, cough inspiratory (CT I ) and expiratory (CT E ) dura- tions were obtained from the mov...

Ngày tải lên: 13/08/2014, 10:20

... genes were functionally clustered into specific biological processes from the classification systems of the gene ontology annotation [35]. The gene ontology analysis of the identified genes ... One-Cycle cDNA Synthesis Kit (Affymetrix). Fifteen micrograms of labeled and fragmented cRNA were subsequently hybridized to a Human Genome U133 plus 2.0 microarray (Affymetrix)....

Ngày tải lên: 31/10/2012, 15:28

... as the high- affinity peak of wild-type LPL. The LPL in the eluate was detected by Western blot with LPL polyclonal antibody 7640. The fraction number of the high-affinity peak was the same for the ... orientation, the catalytic site in the N-terminal domain of one subunit and the substrate recognition site in the C-terminal domain of the other face each other, t...

Ngày tải lên: 21/02/2014, 15:20