Báo cáo y học: "Analysis of cell-based RNAi screens" pdf

... used by the software in the normalization, Analysis steps for a cell-based HTS assayFigure 2 Analysis steps for a cell-based HTS assay. The main steps in the computational analysis of a cell-based ... genes. More generally, one can consider any type of previously known gene list, which we term a category, and ask whether the genes of a category exhibit particularly extreme phenot...

Ngày tải lên: 14/08/2014, 17:22

... previously described [20]. Analysis of Fc γ RIIIa gene polymorphism The analysis of the F/V polymorphism was performed essentially as previously described [21]. MBL gene polymorphism Variants of MBL ... minority of the patients had previously been typed with a lymphocytotoxicity test or by restriction fragment length polymorphism as described before [2]. C4A gene deletion was determin...

Ngày tải lên: 09/08/2014, 01:24

... (white) of each codon of productively rearranged V L genes of B cells isolated from peripheral blood and from the parotid gland of a patient with Sjögren’s syndrome. The frequency of mutation of each ... 400 mg hydroxychloroquine and 2 mg prednisone daily at the time of analysis. After developing parotid gland enlargement, lymphoma of the parotid gland was excluded by partial...

Ngày tải lên: 09/08/2014, 03:24

... ultimately be required. The FcγRs play important roles in the initiation and propaga- tion of many different immunological and inflammatory proc- esses. Consequently, they may act as susceptibility ... participated in the collection of clinical data and the recruitment of patients into the study. DS, JR and VK undertook some of the genotyping assays on DNA prepared in the laboratory...

Ngày tải lên: 09/08/2014, 07:20

... results of a preliminary canine- specific whole genome microarray study, using a small number of samples. Assays were designed for quantification of expres- sion of five collagen genes (type I ... followed by 40 cycles of 95°C for 1 minute and 60°C for 15 seconds, as recommended by the manufacturer (Applied Biosystems). Real-time data were ana- lysed by using the Sequence Detection...

Ngày tải lên: 09/08/2014, 08:22

... University of Pavia, Via Taramelli 5, 27100 Pavia, Italy 2 Department of Biochemistry, University of Pavia, Via Taramelli 5, 27100 Pavia, Italy 3 Department of Internal Medicine, University of Pavia, ... patients. Cystatin SN is a secreted protein, which belongs to SD-type or type-2 cystatins [31], which are potent inhibitors of CA clan mammalian cysteine peptidases. Intracel- lu...

Ngày tải lên: 09/08/2014, 08:22

... phenotypic analysis of lymphoid populations was per- formed. As expected, all MRL mice had expanded lymphocyte populations, primarily in CD4 - CD8 - double-negative T cells. By flow cytometry, ... loss of the major negative regulator of the classical complement pathway fails to impact disease severity, the use of the MRL mouse as a prototypical model of immune complex renal injury...

Ngày tải lên: 09/08/2014, 10:21

... use of the T4 Gene 32 Protein, which may increase the yield of PCR products [30- 32]. Sequence analysis of the PCR-positive samples revealed the presence of a mixture of bacterial DNA in synovial ... with SAPHO (synovitis, acne, pustulosis, hyperostosis, and osteitis) syndrome, which is often regarded to be a form of spondyloarthropathy, suggest- ing an infectious origin of this...

Ngày tải lên: 09/08/2014, 10:23

... 0.0006). Genotype analyses of all of the other SNPs yielded findings similar to those of the allele frequency comparisons, and no differences were found by sex stratification in any of them (not shown). ... polymorphism that is tagged by some haplotypes containing the T allele of rs582757 (Table 2). No significant change was detected by stratifying by sex (data not shown) or by the...

Ngày tải lên: 09/08/2014, 14:20

... 145) and age-matched healthy women (n = 257). XCI analysis was performed by enzymatic digestion of DNA with a methylation sensitive enzyme (HpaII) followed by PCR of a polymorphic CAG repeat in the ... ± 4 years in GD patients). The mean age of diagnosis was 37.9 ± 15.1 years. The diagnosis of GD was based on the presence of biochemical hyperthyroidism as indi- cated by a decrease...

Ngày tải lên: 09/08/2014, 14:22