Báo cáo y học: " Status of complete proteome analysis by mass spectrometry: SILAC labeled yeast as a model system" ppt
Báo cáo y học: " Status of complete proteome analysis by mass spectrometry: SILAC labeled yeast as a model system" ppt
... Issue 6, Article R50 Research Status of complete proteome analysis by mass spectrometry: SILAC labeled yeast as a model system Lyris MF de Godoy *† , Jesper V Olsen *† , Gustavo A de Souza *† , ... pairs. Yeast was SILAC labeled as explained in Figure 7 and one gel band was analyzed. In principle, SILAC peptide pairs should both be recognized and sequ...
Ngày tải lên: 14/08/2014, 16:21
Báo cáo y học: "Extension of the PNA world by functionalized PNA monomers eligible candidates for inverse Diels Alder Click Chemistyr"
... evaporator and con- secutively purified by a silica gel column. As an eluent n-hexane and acetic ether were used at the ratio of 2:1. The purity was estimated by use of thin-layer chromatography. Cleavage ... continuously over night and concentrated by the rotary evaporator. The yellow residue 15 featured oily consistency and was dissolved in dichloromethane. The solvent wa...
Ngày tải lên: 26/10/2012, 08:57
Báo cáo Y học: Characterization of the exopolysaccharide produced by Streptococcus thermophilus 8S containing an open chain nononic acid doc
... Center, Department of Biomolecular Mass Spectrometry) essentially according to [13]. Argon was used as a collision gas with a collision energy of 75 eV. Monosaccharide and methylation analysis For ... methanol, the mixture of partially methylated alditols was acetylated with acetic anhydride (3 h, 120 °C), and analyzed by GLC and GLC–EIMS [14,19]. NMR spectroscopy Prior to N...
Ngày tải lên: 08/03/2014, 09:20
Báo cáo Y học: Regulation of mammalian translation factors by nutrients pot
... kinases although mTOR has not been shown to phosphorylate any lipid substrates. Similar domains are also found in several protein kinases such as the ataxia talengiectasia (mutated) kinase ATM and ... importantly, they found that a mutant of S6K1 that is resistant to inhibition by rapamycin was also resistant to the effects of amino acid withdrawal. These data suggest that amino aci...
Ngày tải lên: 23/03/2014, 21:20
Báo cáo Y học: Inhibition of SERCA Ca2+ pumps by 2-aminoethoxydiphenyl borate (2-APB) 2-APB reduces both Ca2+ binding and phosphoryl transfer from ATP, by interfering with the pathway leading to the Ca2+-binding sites ppt
... Activities of the Ca 2+ ATPase were measured at 37 °C, using the coupled enzyme assay, at either pH 7.2 (A) or pH 6.0 (inset). The activity of purified Ca 2+ ATPase was also measured as a function of ... effects of 2-APB on the activity of the purified Ca 2+ -ATPase were investigated, were carried out using a coupled enzyme assay as previously described [22]. Typically, 15 lgo...
Ngày tải lên: 31/03/2014, 23:20
Báo cáo y học: "Adaptation of the Emotional Contagion Scale (ECS) and gender differences within the Greek cultural context" ppt
... with a mean age of 19.9 years (standard deviation (SD) = 3.28 years) and 312 men with a mean age of 20.76 years (SD = 3.50 years). The age span for both men and women was 18 to 45 years. The sample ... file [http://www.biomedcentral.com/content/supplementary/1744- 859X-7-14-S1.doc] BioMed Central Page 1 of 6 (page number not for citation purposes) Annals of General Psychiatry Open...
Ngày tải lên: 08/08/2014, 23:21
Báo cáo y học: "Reliability of computerized image analysis for the evaluation of serial synovial biopsies in randomized controlled trials in rheumatoid arthritis" pps
... Reli- ability of measures of disease activity and disease damage in rheumatoid arthritis: implications for smallest detectable dif- ference, minimal clinically important difference, and analysis of ... evaluation of ST. The major advantage of DIA is standardization of image acquisition and processing, minimizing variance, and the ability to quantify the actual stained area toget...
Ngày tải lên: 09/08/2014, 06:23
Báo cáo y học: "Modulation of granulocyte-endothelium interactions by antileukoproteinase: inhibition of anti-type II collagen antibody-induced leukocyte attachment to the synovial endothelium" ppt
... Germany) and transferred to an S-VHS video system for subsequent off-line analysis. Microcirculatory analysis For quantitative off-line analysis a computer-assisted microcir- culation image analysis ... assays (cell adhesion assays, FACS analysis, reporter gene assays) and the statistical analysis. PG and AK performed the animal experiments and intravital fluo- rescence microscopi...
Ngày tải lên: 09/08/2014, 08:22
Báo cáo y học: " Impairment of chondrocyte biosynthetic activity by exposure to 3-tesla high-field magnetic resonance imaging is temporar" pptx
... cartilage) was always kept con- stant [13,14]. The other part of the cartilage samples was used for mRNA isolation, alkaline phosphatase assays, and cell death assays; to this end, tissue was digested ... runx2/cbfa1 (270 bp), 5'-CCCCACGACAACCGCAC- CAT-3' (forward) and 5'-CACTCCGGCCCACAAATC-3' (reverse) [25]; IL-1β (394 bp), 5'-AAA CAG ATG AAG AGC TGC ATC CAA-3&apos...
Ngày tải lên: 09/08/2014, 08:22
Báo cáo y học: "Risk of acute myocardial infarction with nonselective non-steroidal anti-inflammatory drugs: a meta-analysis" ppsx
... myocardial infarction; BMI, body mass index; CABG, coronary artery bypass grafting; CAD, coronary artery disease; CCF, congestive cardiac failure; CHD, coronary heart disease; COX, cyclo-oxygenase; CVD, ... colorectal adenomas and carcinomas. Cochrane Database Syst Rev 2004, 2:CD004079. 8. Cryer B, Feldman M: Cyclooxygenase-1 and cyclooxygenase-2 selectivity of widely used nonsteroidal a...
Ngày tải lên: 09/08/2014, 08:22