Báo cáo y học: "Protein synthesis molecule by molecule" pot

... sizes - a very similar behavior to that observed by Yu et al. [11] for protein production. This similarity immediately leads to a possible alternative interpretation of the results by Yu et al. ... some of the spatial dynamics, as it can in many eukaryotic systems [14]. Although protein localization has previously been addressed using single -molecule immuno- chemistry in fixed specimens...

Ngày tải lên: 14/08/2014, 16:21

... interactions can be inferred: by identifying domain pairs that are significantly overrepresented in inter- acting proteins [72]; by coevolutionary analysis; by identi- fying correlated mutations ... transfer) usually provides very limited, if any, clues. Evolutionary relationships, though complicated by the ortholog/paralog dichotomy, are by far the strongest predictors and the next...

Ngày tải lên: 14/08/2014, 21:20

... reaction followed by synthesis is puzzling or in any case very difficult to analyse in terms of kinetics. From a functional point of view, if a reaction reverse of synthesis followed by synthesis using ... phosphatidylinositol; PtdOH, phosphatidic acid; CDP-DAG, CDP-diacylglycerol; CMP, cytidine monophosphate; CTP, cytidine triphosphate. Enzymes: phosphatidylinositol synthase or CDP-...

Ngày tải lên: 22/02/2014, 04:20

... synoviocytes [37]. The present study demonstrates the potency of IL-17, IL-1β, and TNF-α alone and also in combination to induce the syn- thesis of IL-6, IL-8, and TSG-6 by SFCs. The three cytokines activate ... Extraction Kit; Qiagen, Hilden, Germany) followed by transcription in vitro by the SP6 promoter with the Roche transcription system. The recombinant RNA was quantified by the...

Ngày tải lên: 09/08/2014, 01:22

... of cytokines and endog- enous proinflammatory molecules. High mobility group box chromosomal protein 1 (HMGB1), a nuclear DNA-binding protein, proved recently to be a potent proinflammatory cytokine ... O, Erlandsson-Harris H, Janson A, Kokkola R, Zhang M, Yang H, Tracey KJ: High mobility group 1 protein (HMG-1) stimulates proinflammatory cytokine synthesis in human monocytes. J Exp Med 200...

Ngày tải lên: 09/08/2014, 10:23

... Chem Neuroanat 2005, 29:127-136. 34. Hayashi Y, Nishio M, Naito Y, Yokokura H, Nimura Y, Hidaka H, Watanabe Y: Regulation of neuronal nitric-oxide synthase by calmodulin kinases. J Biol Chem 1999, ... of nNOS [35]. Recently, Xu and Krukoff demonstrated in an in vitro study that ADM significantl y stimulated NO production from primary rat hypothalamic neurons by dephosphorylation of...

Ngày tải lên: 10/08/2014, 05:21

... down- regulation of PLC activity [4] and intracellular metabo- lism of IP 3 by phosphomonoesterases [5-8]. Although PLC activity is modulated by depletion of enzyme substrate [4], and decay of receptor-mediated ... removed by sequen- tial sedimentation with 3% gelatin in order to remove most of the erythrocytes. Following centrifugation (280 × g at 10°C for 10 min), residual erythrocyte...

Ngày tải lên: 11/08/2014, 08:22

... after Fontan surgery • PLE may be the common endpoint of multiple patho- physiological pathways. We hypothesise that infection and inflammation are associated with, and may possi- bly be, an important ... were quantified by ELISA (R&D Systems, Wiesbaden, Germany and IBL GmbH, Hamburg, Germany, respectively). The concentration of the complement fragment C3d was determined by radio- imm...

Ngày tải lên: 12/08/2014, 19:22

... analysis of CDK9 dephosphorylation by PP1 and PP2A in vitro. Autophos- phorylated CDK9/cyclin T1 was subjected to dephospho- rylation by PP2A and PP1. Also we analyzed the effect of dephosphorylation ... phosphorylation of CDK9. Dephosphorylation of CDK9 by PP2A prevents formation of P-TEFb/Tat/TAR RNA complex in vitro We next analyzed whether dephosphorylation of CDK9 by PP2A or by...

Ngày tải lên: 13/08/2014, 09:21

... 3,5,3',5'-tetraiodo-l-thyronine, 6,7-dinitroquinoxaline- 2,3-dione, 9-hydroxy aristolochic acid, 3,5,7-trihydroxy-2-(4- hydroxyphenyl)-4h-chromen-4-one, 5-hydroxy-2-(4-hydrox- yphenyl)-1-benzofuran-7-carbonitrile, ... calculated, of which 13 were calculated by Sybyl (Tripos Inc., St Louis, Mis- souri, USA [46]), 49 by Cerius2 (Version 4.10L, Accelrys Inc., San Diego, CA, USA [47...

Ngày tải lên: 14/08/2014, 08:20