Báo cáo y học: "RNA interference pinpoints regulators of cell size and the cell cycle" doc

... Drosophila cell cultures treated with double-stranded RNA were analyzed by flow cytometry, providing a wealth of new information and identifying 488 regulators of the cell cycle, cell size, and cell ... detectable pheno- types. Surprisingly though, they primarily affected the cell cycle rather than cell size. Activation of this pathway in vivo, through the muta...

Ngày tải lên: 14/08/2014, 16:21

... the role of RNA in many facets of biology, illustrating the complexity and the dynamic interplay of cellular regulation. As plants and invertebrates lack the protein-based adaptive immunity that ... pathway. The biogenesis of RNA silencing transcripts can be derived from either the host cell nucleus mRNA pathway to yield miRNA or the cytoplasmic double strand RNA...

Ngày tải lên: 13/08/2014, 09:21

... 0.05). Cell viability and cell cycle To observe the effect of the combination of ASsiRNA and rADI i n HeLa and MCF-7 cells, cell viability and cell cycle were analyzed by MTT and flow cytometry, respectively. ... cytometry, respectively. The results of the cell viability (Figure 3) show that only the combination of ASsiRNA and rADI (Figure 3a) significa...

Ngày tải lên: 10/08/2014, 05:21

... by a Grant-in-Aids for Scientific Research from the Ministry of the Education, Culture, Sports, Science, and Technology of Japan. Author details 1 Department of Transfusion Medicine and Cell Therapy, ... Cell Therapy, Kyoto University Hospital, Kyoto, Japan. 2 Division of Internal Medicine, Kyoto Second Red Cross Hospital, Kyoto, Japan. 3 Department of Molecular Cell P...

Ngày tải lên: 10/08/2014, 09:22

... rely on cultured cells of uncertain phenotype and did not address the possibility that fluid transport may involve multiple different epithelial cell types, including alveolar epithelial type ... deficiency of this channel may also affect the function/expression other channels. Poten- tially this may be explained as that ion flow through the channel, either partially or wholly,...

Ngày tải lên: 12/08/2014, 15:21

... (pri-miRNA) and then proc- essed by the nuclear enzyme Drosha into shorter stem- loop structures (pre-miRNAs) that are exported from the nucleus to the cytoplasm, where they are cleaved by Dicer to yield ... expression of large T antigen late in the viral replica- tive cycle [14]. Since SV40 large T antigen is the major tar- get of the host cytotoxic T lymphocyte (CTL) respo...

Ngày tải lên: 13/08/2014, 06:20

... million years since the separation of the genus Drosophila [17]. Their regulation by the RNAi machinery could simply be a reflection of their retrotransposon nature. On the other hand, there is ... increase both the abundance of HeT-A and TART RNA and their frequency of transposition to broken ends [9]. In contrast, loss of one copy of either of the DNA repair ge...

Ngày tải lên: 14/08/2014, 16:21

... splice forms of HIF [14-16]. Specifically, it has been hypothesized that the antisense molecule may destabilize one splice variant of HIF mRNA and shift the balance in favor of the other variant ... artificial RNA duplexes and then transfected them into the cells. To investigate the presence of RNA duplexes in transfected and untreated cells, cytoplasmic lysate was iso...

Ngày tải lên: 14/08/2014, 16:21

... with a secondary reparative process; (3) the location of the impact and the occur- rence of the tumor corresponded exactly one to the other; and (4) there was a more than one year interval between ... or supple- mented by imaging criteria. CT/MRI, which elegantly reveal the severity and location of the traumatic brain injury and the subsequent formation of...

Ngày tải lên: 25/10/2012, 11:48

... decrease the number of handling steps, reduce contamination, and increase throughput and the accuracy of quantification will further enhance the utility of these assays [2,5]. Given the varied technologies ... M, Miyano Y, et al. Emergence and takeover of YMDD motif mutant hepatitis B virus during long-term lamivudine therapy and re-takeover by wild type after cessa...

Ngày tải lên: 03/11/2012, 09:41