Báo cáo y học: "RNA editing of human microRNAs" pdf

... original work is properly cited. Human miRNA editing& lt;p>A survey of RNA editing of miRNAs from ten human tissues indicates that RNA editing increases the diversity of miRNAs and their targets.</p> Abstract Background: ... A-to-I editing sites in six out of 99 pri-miRNAs, indicating that at least 6% of all human miRNAs may be targets of RNA editing. We wer...

Ngày tải lên: 14/08/2014, 16:21

... hibitor of reCBG [the hydrolysis of 4NP-b- D - glucopyranoside (10 m M ) was reduced by 98% in the presence of 4NP-b- D -mannopyranoside (10 m M )]. Hydrolysis of xenobiotic glycosides by reCBG The ... of alkyl glycosides with two human beta-glucosidases. Biochem. J. 262, 541±548. 11. Day, A.J. DuPont, M.S. & R idley, S. (1998) Deglycosylation of ¯avonoid and iso¯avonoid gly...

Ngày tải lên: 08/03/2014, 16:20

... The requirement of cofactor for TDP enzymatic activity was examined by determining the specific activity of TDP by following the cleavage of 1 m M of substrate by 0.125 l M of enzyme in reaction ... as free enzyme for subsequent rounds of catalysis [15]. Fig. 4. Time-dependence of human TDP activity. Enzymatic reactions were performed by incubating 0.125 l M of human TDPND3...

Ngày tải lên: 31/03/2014, 21:21

... anti-TGF-β or IL-10. Adding less than 1% of these cells to T cells strongly inhibited the generation of cytotoxic T-lymphocyte activity by preventing the activation of CD8 + cells [14]. Other workers ... immunosuppression by CD4 + CD25 + regulatory T cells is mediated by cell surface-bound TGF-β [19]. Many of these differences can possibly be explained by the heterogeneity of CD4 +...

Ngày tải lên: 09/08/2014, 03:24

... preferen- tially by Th1 cells and IL-8 mainly by Th2 cells, whereas MCP- 1 is induced equally by both subsets. This illustrates the capacity of fibroblasts to activate flexible patterns of chemok- ine ... inhibitor of the MEK/ERK pathway U-0126 strongly inhibited IL-8 mRNA induced by both Th1 and Th2 cells (Figure 6a,b). Inhibition of nuclear factor-κB (NF-κB) pathway by PSI and of...

Ngày tải lên: 09/08/2014, 07:20

... effect of the combination of ASsiRNA and rADI i n HeLa and MCF-7 cells, cell viability and cell cycle were analyzed by MTT and flow cytometry, respectively. The results of the cell viability (Figure ... use of down-regulation of this enzyme by RNA interference in three human cancer cell lines (A375, HeLa, and MCF-7) as a way to restore sensitivity to rADI in resistant cells. The e...

Ngày tải lên: 10/08/2014, 05:21

... disease mechanism of the early- onset hypertrophic cardiomyopathy and encephalopath The patients of early-onset hypertrophic cardiomyopathy and encephalopathy were shown to have a homozygous mutation, ... similar- ity analyzed by EMBOSS Pairwise Alignment Algorithms [43], though there is h igh conservation between human NDUFV2 and Y. lipolytica NUHM with 51.8% identity and 66.5% simila...

Ngày tải lên: 10/08/2014, 05:21

... Interactions of human BMMSCs and F-MSCs with leukocyte subpopulations 3.1 Interactions with T Lymphocytes Currently, the interactions of MSCs with T lymphocytes are the best studied. Many reports ... produc- tion of IL-12 by DCs. In the presen ce of BMMSCs, the differentiation of CD14 + monocytes into DCs is impaired, and the monocytes retain high expression of CD14 + –a marker...

Ngày tải lên: 10/08/2014, 05:21

... constituted by all elements except genomic DNA; and the positive control was constituted by HPV-DNA type 16 previously typing (Diagnosis Molecular Laboratory of University of Caxias do Sul). Four μg of ... pairs (pb) of the human β-globin gene, ensuring the qualification and quantification of DNA for HPV analysis, and by the PGM09 and PGMY11 oligonucleotides, which amplify a segme...

Ngày tải lên: 12/08/2014, 04:21

... with adenovirus types 4, 7 and 21 in military recruits during a 9-year survey. J Hyg (Lond) 1969, 67:255-268. 20. Morgan PN, Moses EB, Fody EP, Barron AL: Association of adenovirus type 16 with Reye’s-syndrome-like ... 11:116 http://respiratory-research.com/content/11/1/116 Page 4 of 8 15. Ryan MA, Gray GC, Smith B, McKeehan JA, Hawksworth AW, et al: Large epidemic of respiratory illnes...

Ngày tải lên: 12/08/2014, 11:22