... amplified, cloned, sequenced, and identified. Recombinant adenoviruses encoding rat BMP4 and BMP6 were constructed and compared with ADhBMP4 and ADhBMP6 in the in vitro and in vivo models. At the ... was detected in ADNULL-transduced cells. Figure 3 Western blot detection of BMP4 (A), BMP6 (C and E), and β-actin (B and D) . A solution of lyzed transduced Vero cells (A, B, C, and D) or conditioned ... pAdEasy1 probe. B. BMP6 cDNA fragment probe. DNA samples were digested by HindIII (Lanes 1, 2 and 3), BstXI (Lanes 4, 5, and 6), and BglII plus EcoRV (Lanes 7, 8, and 9). M, 1-kb DNA ladder. Lanes...