Báo cáo y học: "Challenging the spliceosome machine" pot

... sites. The enhancement of A content in the vicinity of D-1 to D6 may reduce the likelihood of RNA secondary struc- ture and thereby facilitate spliceosome function by increasing the availability of ... to this hypothesis, our results suggest that when the spliceosome processes pre- mRNAs with either very long or very short splice elements, it is advantageous to increase the st...

Ngày tải lên: 14/08/2014, 16:20

... delivery of vital substrates. Especially in settings of septic shock, the current routine administration of adrenergic vasopressor therapy therefore may fail to reverse the primary defect. The largely ... of arterial pressure, they may not favorably affect capillary perfusion and, thereby, mitigate the failure of oxygen and other sub- strate delivery and exchange. In the present iss...

Ngày tải lên: 13/08/2014, 18:22

... moved by chromatin-remodeling enzymes and the transcription machinery. In this way, access to the underlying DNA is regulated [38]. Measuring epigenome dynamics Given the evidence that many regions ... spectrometry (MS) can be used to study the dynamics of post- translational modications in the total histone pool by brie y labeling newly synthesized histones with a heavy isotope...

Ngày tải lên: 09/08/2014, 22:23

... integrity – how do you balance your own safety and your patient’s needs? Imagine the devastating psychological distress when you must deny patients access to the hospital for relatively urgent tests ... ethical and psychologic issues – the ‘collateral damage’ of the outbreak – cannot be ignored because they challenge some deeply held convictions and ethical conceptions in ways that th...

Ngày tải lên: 12/08/2014, 19:22

... anticipated. These factors significantly reduced the power of the study. Based on their findings, the authors estimate that more than 100 hospitals would be needed to show the 30% difference they sought. ... seen from baseline to the study period for both groups combined. Conclusion The MET system greatly increases emergency team calling, but does not substantially affect...

Ngày tải lên: 25/10/2012, 10:39

... series will probably be familiar, it is hoped that there will still be useful lessons to be learned, for example the underlying assumptions of a hypothesis test that were not fully appreciated, or ... some previously unrecognised confusion between terms. The first article, presented in this issue, covers the presentation and summary of data. It is unlikely that the material covered by...

Ngày tải lên: 25/10/2012, 10:45

... the catalytic efficiency of the enzyme, the cold adaptation of the enzyme could not be attributed to the presence of the Gly cluster in this element. The present study supports the idea that the ... Gly261 by Ala in mutant G261A resulted in an enzyme with slightly lower stability. On the other hand, in the double mutant G261A /Y2 69A the additional replacement of Tyr269...

Ngày tải lên: 22/02/2014, 04:20

... guanidine hydro- chloride (GdnHCl), urea or elevated temperatures, they destroy the tertiary structure of proteins which results in the loss of enzymatic activity. Regarding the secondary struc- ture ... secondary structure only after the disruption of the native tertiary structure of RNase A (Figs 2–4). Interestingly, the preservation of the tertiary structure coincides with t...

Ngày tải lên: 22/02/2014, 07:20

... angle almost typical for the 3 10 helix. In this region of the polypeptide chain, the secondary structure is characterized also by the O i H N i þ 3 hydrogen bonds, found in all 40 DYANA structures ... residue for the final family is given in Fig. S1. Fig. 3A shows the family of 40 structures and Table 1 contains a summary of statistical data indica- ting the quality of the 40...

Ngày tải lên: 17/03/2014, 10:20

... catalytic activity proceed essentially by the same mechanism. Indeed, catalytic residues occupy the same position in the 3D structures of the two enzymes. In addition, the p redicted secondary structures ... function, display only one polar cavity in the vicinity of the conserved catalytic Asp re sidue. Keywords: neurospora; P-ATPase; homology model; cavity; H + . The 3D struc...

Ngày tải lên: 23/03/2014, 21:20