Báo cáo y học: "Variable window binding for mutually exclusive alternative splicing" pdf

... R2 Research Variable window binding for mutually exclusive alternative splicing Dimitris Anastassiou, Hairuo Liu and Vinay Varadan Address: Center for Computational Biology and Bioinformatics, and Department ... mechanism called Variable Window Binding for mutually exclusive alternative splicing is described for exon 6 of the Drosophila Dscam gene, which represents...

Ngày tải lên: 14/08/2014, 16:20

... mutagenesis; squalene cyclase; thiol reagents. Oxidosqualene cyclases (OSCs) and squalene-hopene cyclases (SHCs) are key enzymes in triterpenoid biosynthesis: they transform acyclic isoprenoid precursors ... active centre cavity by site-directed mutagenesis, able to serve as a Ôsticky pointÕ for squalene analogues with thiol-modifying activity. The D376C/C435S mutant, already used in crysta...

Ngày tải lên: 17/03/2014, 23:20

... Xie, Youzhou and Fu, Yaoyao for their helpful technical instructions. And we feel grateful to all the authors for their citations in the review. Author details 1 Department of Otolaryngology, Eye ... ENT Hospital of Fudan University (formerly Shanghai Medical University), Shanghai, China. 2 Department of Otolaryngology, Eye and ENT Hospital, Fudan University, Shanghai, China. Authors’ con...

Ngày tải lên: 12/08/2014, 04:21

... and fluorescently labeled the cDNA with Cy5 (for the tunicamycin-treated sample) and Cy3 (for the untreated sam- ple) before simultaneous hybridization to genomic microar- rays. mRNAs which are ... puri- fication spin column and hybridized at 50°C for 24 h to glass slide microarrays containing the entire yeast genome. Syn- thesis of the cDNA for poly(A) + RNA (2 µg) used for the expe...

Ngày tải lên: 14/08/2014, 14:21

... 30 min, 50 °C; 1 cycle: 2 min, 94 °C; 35 cycles: 30 s, 94 °C, 30 s, 55 °C; 1min,72°C, followed by 1 cycle for 10 min at 72 °C. For the detection of b-actin, the number of cycles was reduced to 32. ... activity was normalized to b-galactosidase activity. Basal activity of the reporter plasmid in presence of empty expression vector was set to 1. Ó FEBS 2002 E2F6b, an alternatively spliced E...

Ngày tải lên: 08/03/2014, 09:20

... - ferent isoforms could be unambiguously identified by mass spectrometry (MS). A larger scale systematic ana- lysis of isoform proteomic identification based on MS data performed for the fruit fly [10] ... identify those isoform regions that are spe- cific for one isoform, that is, that do not map to any other isoform of the same gene [14]. Leoni et al. Genome Biology 2011, 12:R9 http://g...

Ngày tải lên: 09/08/2014, 22:23

... Fujii M, Hinz M, Nakayama K, Yamada Y, Ikeda S, Yamasaki Y, Kashanchi F, Tanaka Y, Tomonaga M, Yamamoto N: Activation of cyclin D1 and D2 promoters by human T-cell leukemia virus type I tax protein ... by co-cultivating normal human cord leukocytes and human leukaemic T cells. Nature 1981, 294:770-771. 4. Yamamoto N, Okada M, Koyanagi Y, Kannagi M, Hinuma Y: Trans- formation of human leu...

Ngày tải lên: 13/08/2014, 09:21

... model for translocation of Y- family DNA polymerases Ping Xie Correspondence: pxie@aphy.iphy. ac.cn Key Laboratory of Soft Matter Physics and Beijing National Laboratory for Condensed Matter Physics, ... E n -E n+1 ≥ 0 for the Y- family Pol. For the replicative Pol, E’ 1 -E 1 repre sents the binding affinity of binding site S 1 for one base of the ssDNA template (or the bindi...

Ngày tải lên: 13/08/2014, 16:20

... thresholds used for the COS(WR) function for each binding site motif. Tables S4 and Table S5 display detailed results for the enrichment tests performed for AAs, CEs, and ADs, applying the COS(WR) ... alternative events, when applying Single Scores (S) only.Click here for fileAdditional data file 3Experimentally defined SFBSs used for training and testingExperimentally defined...

Ngày tải lên: 14/08/2014, 21:20

... database for eukaryotic transcrip- tion factor binding profiles. Nucleic Acids Res 2004, 32:D91-D94. 21. Montgomery SB, Griffith OL, Sleumer MC, Bergman CM, Bilenky M, Pleasance ED, Prychyna Y, Zhang ... Biotechnology (CeBiTec), Bielefeld University, Universitätsstraße 27, 33615 Bielefeld, Germany. § Institute for Genome Research and Systems Biology (IGS), Center for Biotechnology...

Ngày tải lên: 14/08/2014, 21:20