Báo cáo y học: "Towards the visualization of genome activity at nanoscale dimensions" potx

... achieved by inhibiting the fluorescence of molecules at the outer region of a scanning excitation spot in a saturated manner. With the use of carefully chosen dyes and focal intensity conditions, ... calibration (that is, by spectral precision distance microscopy (SPDM) and spatially modulated illumination (SMI)). For co-localization studies in fixed cells, the distance betwee...

Ngày tải lên: 14/08/2014, 16:20

... a primary phase directly after the start of treatment and a secondary phase that develops in initial responders during the course of therapy. The latter is explained by the formation of anti-drug antibodies ... that TNF-blocking therapy is effective for only approxi- mately two thirds of patients, reflecting that there are ‘responders’ as well as ‘nonresponders’. Given the d...

Ngày tải lên: 09/08/2014, 14:21

... GTTGCtATGC-AAAAATATTATGGCTTTACTAAAATTTATACAAG CATGTCAA TTAACAC Tenrec GTTGCCATaC-AAAAATATTATGGCTTTACTAAAATTTATACAAG CATGTCAA TTAACAC Opossum GTTGCCATGC-AAAAATATTATGGCTTTACTAAAATTTATACAAG CATaTCAA ... GTTGCCATGC-AAAAATATTATGGCTTTACTAAAATTTATACAAG CATGTCAA TTAACAC Rhesus GTTGCCATGC-AAAAATATTATGtCTTTACTAAAATTTATACAAG CATGTCAA TTAACAC Mouse GTTGCCATGC-AAAAATATTATGGCTTTACTAAAATTTATACAAG CgTGTC...

Ngày tải lên: 14/08/2014, 07:21

... clearly on a journey analogous to the one that started 15 years ago and ended with the first draft of the human genome. At last year’s meeting, Richard Gibbs, one of the fathers of the human genome ... warehouses of protein- interaction data. It was good news indeed to hear that they have agreed to cooperate within the International Molecular Exchange (IMEX) consortiu...

Ngày tải lên: 14/08/2014, 08:20

... multimerization on membranes thereby modulating HIV-1 assembly. The formation of the HIV-1 RNP is dynamic and likely central to the fate of the vRNA during the late phase of the HIV-1 replication cycle. Results: ... expectation because packaging into virions of the virus-specific RNA harbor- ing this deletion was less than 2% of that of the wild-type virus [50]....

Ngày tải lên: 12/08/2014, 23:23

... can examine the effect of cross-hybridization by taking advantage of the experimental design of the Latin Square dataset. For each of the 91 possible pairs of experiments in the Latin Fitting data to ... On the other hand, θ 2 is an estimate of the standard error of every gene on the array. We take as our θ 2 simply the average of all σ 2 values. That is...

Ngày tải lên: 14/08/2014, 07:21

... explanations for the failure of this study to demonstrate a benefit for MET. The mostly likely explanation was that the study was underpowered. The initial power and sample size calculations ... While we cannot definitively say that MET systems improve outcomes, it seems self-evident that the goal of identifying and treating patients early in the course of their illnes...

Ngày tải lên: 25/10/2012, 10:39

... efficiency of the enzyme, the cold adaptation of the enzyme could not be attributed to the presence of the Gly cluster in this element. The present study supports the idea that the Gly cluster, in ... compared to the wild-type enzyme). Interestingly, the activity of the mutant at elevated temperatures (20–25 °C) exceeded that of the wild-type protein. Furt...

Ngày tải lên: 22/02/2014, 04:20

... col- lapse of the tertiary structure of RNase A coinciding with the loss of its enzymatic activity. In contrast to the dena- turation by guanidine hydrochloride, urea or temperature, the breakdown of the ... indicate a detectable increase in the content of secondary structure only after the disruption of the native tertiary structure of RNase A (Figs 2–4). Inter...

Ngày tải lên: 22/02/2014, 07:20

... and the wild-type FNRs. Thus, the k cat and K Fd m steady-state parameters and the k obs for the fast ET processes with Fd suggest that in the case of the E139Q mutant formation of the alternative ... FNR mutants at E139 were determined for two reactions catalysed in vitro by FNR by fitting the experimental data to the Michaelis–Menten equation. Diaphorase activity. T...

Ngày tải lên: 31/03/2014, 08:20