Báo cáo y học: " Epigenetic regulation: DNA confers identity but is not enough to maintain it" pps

... deamination of cytosine or 5-methyl cytosine, respectively. This potentially makes TDG part of a 5-methyl cytosine disassembly line, downstream of enzymes such as activation-induced cytidine deaminase ... thymidine DNA glycosylase (TDG), unlike most DNA glycosylases, is essential for embryonic develop- ment. TDG removes the pyrimidines from G:T or G:U mis- matches that occur by deaminat...

Ngày tải lên: 14/08/2014, 16:20

... steps yet very little is known about the mecha- nisms by which chromatin modifications regulate transcrip- tion. Ultimately, structural analysis may provide the key mechanistic insights. This meeting, ... example, the histone acetyltransferase MOZ (monocytic leukemia zinc finger protein) is fused to the histone acetyltransferase CBP. David Heery (University of Nottingham, UK) revealed t...

Ngày tải lên: 14/08/2014, 14:21

... PII-ATP/ GlnK-ATP, 5-carboxymethyl-2-hydroxymuconate iso- merase and 4-oxalocrotonate tautomerase bound to the inhibitor 2-oxo-3-pentynoate. Keywords: nitrogen regulation; PII X-ray structure; crystal packing, ... regulatory subunit of aspartate transcarbamylase, a viral transcriptional regulator and procarboxypeptidase B [9]. Additionally, we found HsPII aligned with the enzymes: 5-carboxy...

Ngày tải lên: 24/03/2014, 04:21

... erythematosus: the Toronto Risk Factor Study. Arthritis Rheum 2003, 48:3159-3167. 34. Selzer F, Sutton-Tyrrell K, Fitzgerald SG, Pratt JE, Tracy RP, Kuller LH, Manzi S: Comparison of risk factors ... levels not only are determined by MBL genotype and promoter poly- morphisms but also are influenced by MASP activity and serum levels of other complement factors. Assays are available to te...

Ngày tải lên: 09/08/2014, 08:23

... appropriately controlled genetic conditions, our results conclusively demonstrate that EGFR activity is not required to produce and is not a major mediator of abnormal activity phenotypes, and ... experimentally manipulated, with infusion of TGFA into the hamster hypothalamus reversibly suppressing locomotor activity [3,6]. However, exogenous administration of receptor lig- ands can...

Ngày tải lên: 10/08/2014, 09:20

... Densitometric values are always pre- sented as a ratio of chemokine receptor band intensity to GAPDH band intensity. Flow cytometric analysis In preparation for flow cytometric analysis, freshly iso- lated ... donor. Microarray analysis Gene expression analysis was performed using Affymetrix U95Av2 gene arrays, according to the manufacturer's pro- tocols. Ten micrograms of total RNA...

Ngày tải lên: 11/08/2014, 08:21

... power to detect CAP patients that will be hospitalised, whereas CRP or WBC have very poor discriminatory power in this point. In CAP it is essential to assess disease severity to optimise therapeutic ... leukocyte count Leukocyte count (WBC) was determined by the hospital laboratory. Serum CRP was measured by nephelometry with a commercially available assay (Behring Diagnostics, Marbu...

Ngày tải lên: 12/08/2014, 14:20

... nurses and physicians – significantly over-rating their performance as to protocol adherence [12]. Even if analgesia was monitored adequately and medications administered according to need, the ... multiple barriers exist that mitigate against the perfect management of pain in the ICU, the first step is to measure the pain and to measure the response to initial therapy. Without th...

Ngày tải lên: 13/08/2014, 10:20

... array (CBA) multiplex assay (BD Biosciences, San Jose, CA, USA). Histology and immunohistochemistry Paraffin lung sections were stained with haematoxylin and eosin or fluorescein isothiocyanate-labeled ... were measured by ELISA. Plasminogen activator activity (PAA) was determined by an amidolytic assay [26]. Tumor necrosis factor (TNF)-α, IL-6, IL-12p70, IL-10 and interferon (IFN)-γ were mea...

Ngày tải lên: 13/08/2014, 20:21

... both adrenal tissue and Y1 adrenal tumor cells by immunohistochemistry and immunocytochemistry and Western blot. Stimulation of Y1 cells with ACTH significantly stimulated endogenous mitochondrial ... (2001) Mitochondrial processing of newly synthesized steroidogenic acute regulatory protein (StAR), but not total StAR, mediates cholesterol transfer to cytochrome P450 side chain cleavag...

Ngày tải lên: 08/03/2014, 09:20