Báo cáo y học: "Functional genomics of early cortex patterning" ppt

... mammalian cortex. (a) Dorsal view of the mouse cerebral cortex at day E13.5 with the rostro-caudal gradients of the transcription factors Pax6 and Emx2 superimposed (see [9]). (b) The early cortex ... cortical patterning by signaling in the direction of the arrows (reviewed in [12]). (d) Sansom et al. [5] interrogated the genetic identity of the neocortex by performing a microa...

Ngày tải lên: 14/08/2014, 16:20

... studying DNA damage Early studies using DNA microarrays indicated that transcrip- tional responses often reflect underlying biology: for instance, the expression of cell-cycle genes that cycle ... as a system are motivated by these factors, but efforts to do this are limited by tech- niques, by resource availability and perhaps by our concep- tualization of the nature of the biology....

Ngày tải lên: 14/08/2014, 17:22

... One-Cycle cDNA Synthesis Kit (Affymetrix). Fifteen micrograms of labeled and fragmented cRNA were subsequently hybridized to a Human Genome U133 plus 2.0 microarray (Affymetrix). After the hybridization, ... 1. Laboratory for Molecular Pathobiochemistry and Clinical Research, Max Planck Institute of Molecular Physiology, Dortmund, Germany; 2. Department of Statistics, University...

Ngày tải lên: 31/10/2012, 15:28

... wild- type or heterozygous for the Y2 38X polymorphism. Cells ho mozygous for the DECTIN-1 Y2 38X poly- morphism were obtained from three members of a family previously analyzed for mucocutaneous ... presence of the DECTIN-1 Y2 38X polymorphism on cytokine production capacity of macrophages were studied by stimulating the cells with b-glucans. Furthermore, the presence of t he DECT...

Ngày tải lên: 12/08/2014, 11:23

... potentiated by enzymatic degradation of phospholipids [7,44]. The capacity of DM64 to inhibit the myotoxicity induced by myotoxins I (Asp49) and II (Lys49) from B. asper venom was analyzed in vivo ... three domains and are present in the inhibitory receptors of Fig. 6. Inhibition of in vitro cytotoxicity of myotoxins I or II by DM64. Cytotoxicity was analyzed in vitro using C2C12 sk...

Ngày tải lên: 21/02/2014, 01:21

... lLof4· SDS polyacrylamide gel treatment buffer. Twenty microliters of each sample was then electrophoresed in 12.5% SDS polyacrylamide gels, blotted to nitrocellulose and probed with antibody ... H.S., Han, Y. & Fung, B.K K. (2000) Subunit e xchange of small h eat sho ck proteins. Analysis of olig- omer formation of aA-crystallin and Hsp27 by fluorescence res- onance energy transf...

Ngày tải lên: 22/02/2014, 04:20

... was analysed by FACS. RESULTS Functional expression of a Myc-his tagged version of CCR5, and a his-tagged version of CD4 Because the post-translational modifications of CCR5, such as tyrosine ... obtained by limiting dilution and analysed by FACS using the antibody 2D7 (Fig. 1), which recognizes CCR5 [36]. Western blot analysis of the cloned protein from a postnuclear supernatant of...

Ngày tải lên: 08/03/2014, 09:20

... hibitor of reCBG [the hydrolysis of 4NP-b- D - glucopyranoside (10 m M ) was reduced by 98% in the presence of 4NP-b- D -mannopyranoside (10 m M )]. Hydrolysis of xenobiotic glycosides by reCBG The ... puri®cation of CBG and allowed characterization in s ome detail, especially with respect to its glycone/aglycone speci®city a nd ability to catalyse the hydrolysis of dietary xenobi...

Ngày tải lên: 08/03/2014, 16:20

... .D. (2001 ) Biosynthesis of the linkage region of glycosaminoglycans: c loning and activity of galactosyltransferase II, the sixth member of the b1,3galactosyltransferase f amily (b3GalT6). J. ... and Thierry Hennet Institute of Physiology, University of Zu È rich, Switzerland The b1,3-glycosyltransferase enzymes identi®ed to date share several conserved r egions and c onserved c...

Ngày tải lên: 08/03/2014, 16:20

... System for X-Ray Crystallography and NMR. Yale University Press, New Haven, CT, USA. 43. Jack, A. & Levitt, M. (1978) Refinement of large structures by simultaneous minimization of energy ... completely fit the results of t he double-mutant cycle analysis. Alternatively, the interaction of IL-4 side chain N15 with H159 (coupling energy only 0.8 k JÆmol )1 ) m ay be indirect. Of p...

Ngày tải lên: 08/03/2014, 16:20