Báo cáo y học: "Functional genomics of early cortex patterning" ppt
Báo cáo y học: "Functional genomics of early cortex patterning" ppt
... mammalian cortex. (a) Dorsal view of the mouse
cerebral cortex at day E13.5 with the rostro-caudal gradients of the
transcription factors Pax6 and Emx2 superimposed (see [9]). (b) The
early cortex ... cortical
patterning by signaling in the direction of the arrows (reviewed in [12]).
(d) Sansom et al. [5] interrogated the genetic identity of the neocortex by
performing a microa...
Báo cáo y học: "Functional genomics of the yeast DNA-damage response" ppsx
... studying DNA damage
Early studies using DNA microarrays indicated that transcrip-
tional responses often reflect underlying biology: for instance,
the expression of cell-cycle genes that cycle ... as a system are motivated
by these factors, but efforts to do this are limited by tech-
niques, by resource availability and perhaps by our concep-
tualization of the nature of the biology....
Báo cáo y học: "Functional genomics analysis of low concentration of ethanol in human hepatocellular carcinoma (HepG2) cells. Role of genes involved in transcriptional and translational processes"
... One-Cycle cDNA
Synthesis Kit (Affymetrix). Fifteen micrograms of
labeled and fragmented cRNA were subsequently
hybridized to a Human Genome U133 plus 2.0
microarray (Affymetrix). After the hybridization, ...
1. Laboratory for Molecular Pathobiochemistry and Clinical Research, Max Planck Institute of Molecular Physiology,
Dortmund, Germany;
2. Department of Statistics, University...
Báo cáo y học: "Functional consequences of DECTIN-1 early stop codon polymorphism Y238X in rheumatoid arthritis" pptx
... wild-
type or heterozygous for the Y2 38X polymorphism.
Cells ho mozygous for the DECTIN-1 Y2 38X poly-
morphism were obtained from three members of a
family previously analyzed for mucocutaneous ... presence
of the DECTIN-1 Y2 38X polymorphism on cytokine
production capacity of macrophages were studied by
stimulating the cells with b-glucans. Furthermore, the
presence of t he DECT...
Tài liệu Báo cáo Y học: Functional analysis of DM64, an antimyotoxic protein with immunoglobulin-like structure from Didelphis marsupialis serum pdf
... potentiated by enzymatic degradation of
phospholipids [7,44].
The capacity of DM64 to inhibit the myotoxicity induced
by myotoxins I (Asp49) and II (Lys49) from B. asper venom
was analyzed in vivo ... three
domains and are present in the inhibitory receptors of
Fig. 6. Inhibition of in vitro cytotoxicity of myotoxins I or II by DM64.
Cytotoxicity was analyzed in vitro using C2C12 sk...
Tài liệu Báo cáo Y học: Functional analysis of a small heat shock/a-crystallin protein from Artemia franciscana docx
... lLof4· SDS
polyacrylamide gel treatment buffer. Twenty microliters of
each sample was then electrophoresed in 12.5% SDS
polyacrylamide gels, blotted to nitrocellulose and probed
with antibody ... H.S., Han, Y. & Fung, B.K K. (2000)
Subunit e xchange of small h eat sho ck proteins. Analysis of olig-
omer formation of aA-crystallin and Hsp27 by fluorescence res-
onance energy transf...
Báo cáo Y học: Functional reconstitution of the HIV receptors CCR5 and CD4 in liposomes pot
... was
analysed by FACS.
RESULTS
Functional expression of a Myc-his tagged version
of CCR5, and a his-tagged version of CD4
Because the post-translational modifications of CCR5, such
as tyrosine ... obtained by limiting dilution and analysed by
FACS using the antibody 2D7 (Fig. 1), which recognizes
CCR5 [36]. Western blot analysis of the cloned protein from
a postnuclear supernatant of...
Báo cáo Y học: Functional expression of human liver cytosolic b-glucosidase in Pichia pastoris Insights into its role in the metabolism of dietary glucosides ppt
... hibitor of reCBG [the hydrolysis of 4NP-b-
D
-
glucopyranoside (10 m
M
) was reduced by 98% in the
presence of 4NP-b-
D
-mannopyranoside (10 m
M
)].
Hydrolysis of xenobiotic glycosides by reCBG
The ... puri®cation of CBG
and allowed characterization in s ome detail, especially with
respect to its glycone/aglycone speci®city a nd ability to
catalyse the hydrolysis of dietary xenobi...
Báo cáo Y học: Functional assignment of motifs conserved in b1,3-glycosyltransferases A mutagenesis study of murine UDP-galactose:b-N-acetylglucosamine b1,3-galactosyltransferase-I pptx
... .D. (2001 )
Biosynthesis of the linkage region of glycosaminoglycans: c loning
and activity of galactosyltransferase II, the sixth member of the
b1,3galactosyltransferase f amily (b3GalT6). J. ... and Thierry Hennet
Institute of Physiology, University of Zu
È
rich, Switzerland
The b1,3-glycosyltransferase enzymes identi®ed to date
share several conserved r egions and c onserved c...
Báo cáo Y học: Functional epitope of common c chain for interleukin-4 binding ppt
... System for X-Ray
Crystallography and NMR. Yale University Press, New Haven,
CT, USA.
43. Jack, A. & Levitt, M. (1978) Refinement of large structures by
simultaneous minimization of energy ... completely fit the
results of t he double-mutant cycle analysis. Alternatively,
the interaction of IL-4 side chain N15 with H159 (coupling
energy only 0.8 k JÆmol
)1
) m ay be indirect. Of p...
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