Báo cáo y học: "Systematic analysis of gene expression in human brains before and after death" docx

... may alter gene expression patterns in postmortem human brains. Study of expression levels of 14 genes in human brain autopsy and biopsy samples found significant change in one of the genes, indicating ... protein ubiquitination is significantly increased after death, while the expression of genes involved in protein biosynthesis, rRNA processing, organelle org...

Ngày tải lên: 14/08/2014, 16:20

... patients and 12 controls [18]. The microarray assay used (Panorama Cytokine Gene Array membranes; Sigma Genosys, Inc) included 375 genes enriched in cytokines, chemokines, cell surface 286 ably derive ... together and were preferentially induced by a combination of IFN-α and IFN-β. However, two genes preferentially induced by IFN-γ (SERPING1 and FCGR1A) were also significantly i...

Ngày tải lên: 09/08/2014, 01:23

... microarray analysis identified 209 genes that were differentially expressed in circulating leukocytes from 14 high- versus low-lonely individuals, including up-regulation of genes involved in immune ... activation and prolif- eration. Several other indications of immune activation emerged in the set of over-expressed genes, including pro- inflammatory cytokines, chemokines, gr...

Ngày tải lên: 14/08/2014, 08:20

... studies of the entire human genome are thus likely to expand the number of protein cod- ing genes accordingly. Complementary natural antisense transcripts exert control at many steps of gene expression ... standard hybridization, staining, and wash- ing protocols. The arrays were scanned on an Affymetrix 7G Plus GeneChip scanner, and the signal intensity data were processed us...

Ngày tải lên: 14/08/2014, 08:20

... farthest in describing the anatomy of gene expression and compares data from numbers of individual animals using different methods to assay gene expression. However, the number of genes covered remains ... function and development of circuits in the brain and for genetic manipulation of circuit elements. This level of analysis will be critical to the next stage...

Ngày tải lên: 14/08/2014, 07:21

... closely related family members is an artifact of hybridization. Profile of microRNA expression in the developing mouse brainFigure 3 Profile of microRNA expression in the developing mouse brain. ... Quantification of microarray data using three synthetic RNAs: syn1, syn2 and syn3. Each data point is the average of two independent labelling/hybridization reactions. Probe...

Ngày tải lên: 14/08/2014, 14:21

... clarify the role and pathogenic importance of Th1/Th2 cytokines and neurotrophin release in asthma pathogenesis. Proliferation and survival of mast cells, eosinophils and lymphocytes in the airways ... neurotrophin mRNA and protein expression We next investigated the ability of the proinflammatory cytokine IL-1β to induce neurotrophin expression in HBSMC. At 2.5 h a...

Ngày tải lên: 12/08/2014, 16:20

... (Figure 2c): rapamycin, calyculin A and wiskostatin. The similar- ity in these profiles resulted from inhibition of strains deleted for genes involved in intracellular transport and multidrug resistance ... com- prehensive view of drug-protein interactions. Such results can play an invaluable role in understanding and predicting a compound's clinical effects and in gu...

Ngày tải lên: 09/08/2014, 20:21

... gain and loss both in terms of defining and of detecting them, and failure to recognize gene remnants will inevitably lead to mistaking a situation like that shown in Figure 1b (in which a gene ... pseudogenes and gene remnants (regions recognizable as gene- derived only by synteny and statistically significant sequence simi- larity to the parent gene) . There is th...

Ngày tải lên: 14/08/2014, 17:22

... ribosylation factor-like protein 3 and annexin A3 (ANXA3)), proteins involved in ion transport (such as serotransferrin (TRFE) and hemoglobin subunit beta) and several inflammatory proteins (including ... phosphorylase, pyruvate kinase isozymes, fibrinogen gamma chain, alpha 1B glycoprotein and actin cytoplasmic 1 were identified. BAL proteome analysis of PLCH patients also...

Ngày tải lên: 10/08/2014, 09:22