Báo cáo y học: "Digital drug discovery" pot

... cardiomyocytes were approved for federally funded research under President George W. Bush’s policy of August 2001. The hESC-derived cardiomyocytes beat in culture, and display an action poten- tial ... ideally take only hours to days to run. The NIH is currently allocat- ing funds for technical developments in this field. The application of genomics, bioinformatics and systems biology in dru...

Ngày tải lên: 14/08/2014, 14:22

... antirheumatic drugs on RASFs were determined by MTS (3-[4,5-dimethylthiazol-2-yl]-5-[3-car- boxymethoxyphenyl]-2-[4-sulfophenyl]-2H-tetrazolium) cell proliferation assay (Promega GmbH, Mannheim, Germany). SFs ... anti-inflammatory drug (NSAID) and steroidal anti-inflammatory drug (SAID) response signatures in human chondrocytesNonsteroidal anti-inflammatory drug (NSAID) and steroidal an...

Ngày tải lên: 09/08/2014, 01:22

... data and genotyped as a heterozygote. A genotype was discordant if a heterozygous SNP was called with the sequence data but the genotype was called homozygous on the DNA microarray. For all metrics, ... Materials and methods). Homozygous and heterozygous calls were examined independently using zygosity calls produced by SAMtools. The observed non- reference allele frequency for SNPs, deletion...

Ngày tải lên: 09/08/2014, 22:23

Ngày tải lên: 14/08/2014, 14:21

... from R&D Systems (Minneapolis, MN) and displayed two bands at 18 kD and 23 kD. This difference may be due to dissimilar cell types, which may produce different numbers of glycosylation sites. ... may be expressed by their adenoviral vectors in AN and SD rats, the osteogenic potentials of BMP4 or BMP6 are significantly reduced in immunocompetent rats. This limitation may be caused b...

Ngày tải lên: 31/10/2012, 17:08

... would have very profound implications for human germline gene therapy. Gene transfer into embryos (using pronuclear microinjection or RVVs) is inherently very costly and technically demanding, ... Similarly, the pronuclei in ovine zygotes are very difficult to visualise, due to sharing a very similar refractive index with the cytoplasm; this necessitates the use of top-quality optics, ......

Ngày tải lên: 03/11/2012, 10:01

... 5200 (RLYR) 2 1950 (RLYR) 4 338 (RLYR) 8 48 Dendritic R4 (RLYR) 2 K 3700 (RLYR) 4 K 2 K 1510 (RLYR) 8 (K 2 K) 2 K 1514 Dendritic R8 RLYRKVYG 3950 (RLYRKVYG) 2 K 1420 (RLYRKVYG) 4 K 2 K 610 (RLYRKVYG) 8 (K 2 K) 2 K 112 Tachyplesin 108 928 ... determined accurately because o f its poor solubility in phosphate buffers, this peptide rapidly and quantitatively induced erythrocytes aggregations. Pr...

Ngày tải lên: 22/02/2014, 04:20

... 269) 1555 cells, using a-hydroxy-farnesyl phosphonic acid, showed that Mydj2, in agreement with previous studies [10,30] exists in two forms, a farnesylated and a nonfarnesylated one (data not shown). We ... the farnesylated and the nonfarnesylated forms of Mydj2 protein by Western blotting analysis, using the same anti-dj2 Ig as in immuno- fluorence experiments. As shown in F ig. 5 , Mydj2 do...

Ngày tải lên: 17/03/2014, 17:20

... CO 2 Acetaladehyde fi acetate Glyceraldehyde 3-P fi glycerol Formation of Acetyl-CoA in the cytosol Acetate fi acetyl-CoA (cytosolic) Anaplerotic reaction (cytosolic) Pyruvate (cytosolic) + CO 2 fi ... 6-P EMP-pathway Glucose 6-P fi fructose 6-P (reversible) Fructose 6-P fi 2 glyceraldehyde 3-P Glyceraldehyde 3-P fi phosphoenolpyruvate Phosphoenolpyruvate fi pyruvate (cytosolic) PP-pathway Glucose .....

Ngày tải lên: 31/03/2014, 23:20

... MMP-2, the clinical phenotype is not yet explained. We still do not know precisely what role MMP-2 plays in human biological processes. In vitro MMP-2 can cleave type I and type IV collagens, but ... MMP- 13 lacking this catalytic domain has no enzymatic activity. The MMP-13 –/– mice are fertile, and they grow and survive normally. The major developmental phenotype includes widened growth ......

Ngày tải lên: 09/08/2014, 01:21