Báo cáo y học: "Canonical Wnt signaling: high-throughput RNAi widens the path" pdf

... func- tional redundancy precludes their detection, or that they have a relatively minor role. Alternatively, their effects may have been masked by the presence of Wingless in the primary screen. This ... is little evidence of these other interacting pathways in the dataset. In some cases it is possi- ble that the modulation of Wnt/ ␤-catenin signaling by these pathways is only seen i...

Ngày tải lên: 14/08/2014, 14:22

... minimizing the effect on the homeostasis of other cytokines. The results of the present study demonstrate the power of using heterogeneous cellular data to qualitatively and quantitatively map intermediate ... interval of all the basal values for the particular measurements. Connections were then drawn from the lig- ands that significantly stimulate cytokines to the signalin...

Ngày tải lên: 14/08/2014, 16:21

... question of the physiological consequences of these interactions. How transient or stable are they at both the cytological and the molecular level? What is the temporal correlation between the interchromosomal ... soluble enzymes and cofactors. Yet in recent years there has been a growing appreciation that the nucleus is in fact highly organized. In interphase chromo- somes, DNA i...

Ngày tải lên: 14/08/2014, 15:20

... contained rapidly proliferating epithelium and a relative paucity of fully differentiated pulmonary cell types. Unexpectedly, use of Affymetrix array analysis to study gene expression revealed very high ... normal, but internally they contained highly proliferative, cuboidal epithelium lacking fully differentiated lung cell types. Unexpectedly, microarray analysis and in situ hybridization re...

Ngày tải lên: 06/08/2014, 18:20

... domains of Syk family kinases, ZAP-70 and Syk. The Syk kinases are activated upon binding to phospho-ITAMs and phosphorylation by the SFKs. Once activated, the Syk kinases phosphorylate the critical ... antigen binding, there are many levels of regulation in this signaling pathway. The SFKs themselves are tightly regulated by phosphorylation of their inhibitory C- terminal tyrosine...

Ngày tải lên: 09/08/2014, 01:22

... monocytes to the site of injured endothelium by pro-inflammatory chemokines is a key regulatory component in the forma- tion of an atherosclerotic lesion [16,17]. The monocytes subsequently adhere ... have already been noted by other researchers studying mono- cyte differentiation [27,28,32]. In particular, we show that THP-1 cells rapidly become adherent and their morphol- ogy changes...

Ngày tải lên: 11/08/2014, 08:21

... com- pletely or partially inactive. Importantly, we repeatedly observed that the transactiva- tion property of Tat was usually not affected by the pro- tein purification strategy employed. The protein purification ... purposes) Activation of the cytokine signaling events by the Tat proteinsFigure 6 Activation of the cytokine signaling events by the Tat proteins. (A) Secretion of...

Ngày tải lên: 13/08/2014, 09:20

... of the Scylla and Charybdis genes is induced by hypoxia via the hypoxia-inducible transcription factor HIF. The same induction by HIF and downstream effects on TSC1/TSC2 was reported for the ... inhibitors discovered by these screens, the paralogous pro- teins Scylla and Charybdis. These two function in the insulin pathway downstream of PI 3-kinase and the protein kinase (and ce...

Ngày tải lên: 14/08/2014, 14:21

... site (5'ss, AG/GURAGU), the branchpoint sequence (CURAY), the polypyrimidine (PY) tract (a run of polypyrimidines located between the 3' splice site and the branchpoint), and the 3' splice ... under the curve was normalized to unity. The two regions used in this study are depicted below the curves. The PY tract region consisted of the region from -30 to -3,...

Ngày tải lên: 14/08/2014, 20:22

... background, leaving the cell monol- ayer. DNA stained images were segmented to identify nuclei in the cell monolayer. Mean cell area was calculated as the area of the monolayer divided by the number of ... in this sys- tem, Gap1 and Tsc2, as genetic landmarks to position positive regulators within the pathway. We began by using RNAi to modify the Gap1 phenotype. As expected,...

Ngày tải lên: 14/08/2014, 21:20