... Africa with the majority of the reported outbreaks attributed to serotype O virus. In this study, phylogenetic analyses of the polyprotein coding region of serotype O FMD viruses from Kenya and Uganda ... 2 Phylogenetic relationships of the VP1 coding region of type O East African viruses in comparison to other FMD virus serotypes. Evolutionary relationships of the VP1 c...
Ngày tải lên: 12/08/2014, 04:20
... cre- ated by using serially diluted XMRV plasmid (pcDNA3.1 (-)/VP62). The assay was analyzed by the ABI 7300 Real- Time PCR System using the default thermal cycling conditions for the two-step RT-PCR ... paraformaldehyde and analyzed by flow cyto- metry (BD FACScan). The percentages of GFP-positive cells were measured using CellQuestPro software [14]. Western blot analysis For Wes...
Ngày tải lên: 13/08/2014, 01:20
Báo cáo y học: "Anticancer Activity of the PR Domain of Tumor Suppressor RIZ1"
... over the progression of cancer; and the second question is whether the PR domain alone possesses anticancer activity. In this study, we quan- titatively analyzed the mRNA expression level of ... research grant from the Cancer Research Society of Canada. W. Sun would also like to acknowledge the College of Phar- macy and Nutrition, University of Saskatchewan for the...
Ngày tải lên: 25/10/2012, 11:15
Báo cáo y học: " Experimental ablation of the pancreas with high intensity focused ultrasound (HIFU) in a porcine model"
... During the therapeutic process, real-time estimation of the therapeutic effect was carried out by the computer system through the graphic changes in the target field and the hyperecho of the ... and use of laboratory animals from the Ministry of Science and Technology of the People’s Republic of China. Instruments HIFU ablation was performed with a HIFU tu-...
Ngày tải lên: 25/10/2012, 11:18
Tài liệu Báo cáo Y học: Molecular modeling of the dimeric structure of human lipoprotein lipase and functional studies of the carboxyl-terminal domain docx
... as the high- affinity peak of wild-type LPL. The LPL in the eluate was detected by Western blot with LPL polyclonal antibody 7640. The fraction number of the high-affinity peak was the same for the ... orientation, the catalytic site in the N-terminal domain of one subunit and the substrate recognition site in the C-terminal domain of the other face each other, t...
Ngày tải lên: 21/02/2014, 15:20
Tài liệu Báo cáo Y học: Structural determinants of the half-life and cleavage site preference in the autolytic inactivation of chymotrypsin pdf
... used. RESULTS The enzymatic activity and molecular stability of the mutants The catalytic activity and the molecular stability of the mutants were assayed because a change in either of these parameters ... seen from their inactivation rates in the presence of equimolar amounts of trypsin (Table 2). The cleavage of the interdomain and autolysis loops To follow the...
Ngày tải lên: 22/02/2014, 07:20
Tài liệu Báo cáo Y học: Solution structure of the mEGF/TGFa44250 chimeric growth factor doc
... fits to the set of distances. The main advantages of the procedure are simple: the details of the calibration are precisely and unambiguously defined, it is fully automatic, and the family of calculated ... found. Several of these were identified in previous studies of EGF, and the backbone fold of the chimera is clearly similar to those of the EGF structures....
Ngày tải lên: 22/02/2014, 07:20
Tài liệu Báo cáo Y học: EPR characterization of the mononuclear Cu-containing Aspergillus japonicus quercetin 2,3-dioxygenase reveals dramatic changes upon anaerobic binding of substrates potx
... perturbation of the axial symmetry of the tensor, whereas axial symmetry, i.e. g xx % g yy << g zz is typically found for type 2 sites. The grouping of the g-values and the line-shape of the spectrum ... Vlaardingen, the Netherlands; 2 University of Groningen, Laboratory of Biophysical Chemistry, Groningen, the Netherlands; 3 Department of Molecular Physics...
Ngày tải lên: 22/02/2014, 07:20
Báo cáo Y học: Subsite mapping of the binding region of a-amylases with a computer program ppt
... is not hydrolysed by a-amylases therefore, the products of hydrolysis are always b-glycosides. Selection of these glycosides as substrates has been based on their size (DP 4–10) and good yields ... region of a-amylases with a computer program Gyo¨ ngyi Gye ´ ma ´ nt 1 , Gyo¨ rgy Hova ´ nszki 2 and Lili Kandra 1 1 Institute of Biochemistry, Faculty of Sciences, University of Debr...
Ngày tải lên: 08/03/2014, 09:20
Báo cáo Y học: Functional reconstitution of the HIV receptors CCR5 and CD4 in liposomes pot
... the C-terminus of the receptor which is located on the inside of the cell, we then determined if signal transduction by the protein was affected by this modification. One of the consequences of ... version of CCR5 was present on the surface of transfected cells, and had the expected molecular mass. To test whether the presence of the myc-his tag at the C-ter...
Ngày tải lên: 08/03/2014, 09:20