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Báo cáo y học: "GOToolBox: functional analysis of gene datasets based on Gene Ontology" pptx

Báo cáo y học:

Báo cáo y học: "GOToolBox: functional analysis of gene datasets based on Gene Ontology" pptx

... biologist.Indeed, on the one hand, the GO-Proxy implementation of a gene- to -gene distance calculation based on several GO termsallows the determination of classes consisting of functionallyrelated genes. ... provided the original work is properly cited.GOToolBox: functional analysis of gene datasets based on Gene Ontology<p>Tools are presented to identify Gene Ontology terms that are over- or under-represented ... selection andfitting of ontology level (GO-Diet), statistical analysis of termsassociated with gene sets (GO-Stats), GO -based gene cluster-ing (GO-Proxy), and gene retrieval based on GO annotationsimilarity...
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Báo cáo y học:

Báo cáo y học: " Antibody microarray analysis of cell surface antigens on CD4+ and CD8+ T cells from HIV+ individuals correlates with disease stages" ppt

... therapy [4-8] and lack of activa-tion and expression of CD38 and HLA-DR on CD4+ Tcells correlates with long-term non-progression [9]. Theenumeration of CD4+ T-lymphocytes by flow cytometry isused ... immunophenotyping of CD antigens relies on flow cytometry. Although very reliable, the flowcytometry only allows estimation of 3–6 markers in agiven assay. The recently developed antibody microarraytechnology ... in syncytia formation and virusinfectivity and the increase in its expression on lym-phocytes in HIV infection [40].Interestingly, the expression levels of two NK associatedreceptors on CD8+...
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Báo cáo y học:

Báo cáo y học: "Longitudinal microarray analysis of cell surface antigens on peripheral blood mononuclear cells from HIV+ individuals on highly active antiretroviral therapy" doc

... HIV-infection. Suchinvestigations would be labor-intensive, time-consumingand expensive if done by flow cytometry. Secondly, thedetections of cell surface antigens in our study lay a solidfoundation ... by flow cytometry, this study isthe first to use a cell -based antibody microarray (135 anti-gens) to retrospectively and longitudinally monitor theeffect of antiretroviral therapy on cell surface ... the duration of the therapy usage for microarrayassay and correlation analysis. The 4 time points that hadsimilar duration of therapy for each patient were furtherselected for studying time...
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Báo cáo y học:

Báo cáo y học: "Drawing the tree of eukaryotic life based on the analysis of 2,269 manually annotated myosins from 328 species" ppsx

... DistanceMdBtCafMamRnHsPatMm03.6(distant)(close)No DataMyo1AMyo1BMyo1CMyo1DMyo1EMyo1FMyo1GMyo1HMyo3AMyo3BMyo5AMyo5BMyo5CMyo6Myo7AMyo7BMyo9AMyo9BMyo10Myo15Myo16Myo18AMyo18BMyo19Myo35times mean distancewithin ... 3000HsMyo7ATicMyo22Pf_aMyo23Pf_aMyo27Pf_aMyo26TepMyo25Pf_aMyo24BAcMyo4HsMyo10HsMyo5AHsMyo3ADmMyo15EnMyo17DmMyo21HsMyo16AtMyo8AHsMyo1AHsMhc1LemMyo13HsMyo6HsMyo19IpMyo28DmMyo20HsMyo9AAtMyo11ATgMyo14CeMyo12HsMyo18APhrMyo29IQ ... page)NematodaVertebrata1000962705820921704UrochordataEchinodermataAnthozoaProtostomiaChoanoflagellida0.35Mhc0.30 0.25 0.20 0.15 0.10 0.05 0Myo5Myo27Myo34Myo6Myo30Myo26Myo23Myo14Myo24Myo25Myo20Myo17Myo18Myo32Myo12Myo16Myo21Myo33Myo35Myo1Orphan SequencesMyo19Myo28Myo3Myo9Myo7Myo15Myo10Myo22Myo13Myo8Myo11Myo31Myo4Myo29http://genomebiology.com/2007/8/9/R196...
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Báo cáo y học:

Báo cáo y học: " A single dose of DNA vaccine based on conserved H5N1 subtype proteins provides protection against lethal H5N1 challenge in mice pre-exposed to H1N1 influenza virus" ppt

... cross-protectionmainly relies on the cell-mediated immune responsesinduced by infection.Though the cross-protection provided by infectioncould play a role in alleviating symptoms of H5N1 infec-tion and reducing ... mice.Conclusions: Pre-existing immunity against seasonal influenza viruses is useful in offering protection against H5N1infection. DNA vaccination may be a quick and effective strategy for persons ... 10):2559-2564.29. Epstein SL, Kong WP, Misplon JA, Lo CY, Tumpey TM, Xu L, Nabel GJ:Protection against multiple influenza A subtypes by vaccination withhighly conserved nucleoprotein. Vaccine...
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Báo cáo y học:

Báo cáo y học: "Segment-orientated analysis of two-dimensional strain and strain rate as assessed by velocity vector imaging in patients with acute myocardial infarction"

... artery (LAD) by primary PCI were retrospectively enrolled in this study. Inclusion criteria were AMI caused by LAD oc-clusion (type I, ESC) and coronary artery disease af-fecting only 1 or ... stratification 1. Further intervention, such as a percutaneous coronary inter-vention (PCI) or a coronary artery bypass graft (CABG), is only indicated if the myocardium is hypokinetic due ... Faber L. Two-dimensional strain analysis of the global and regional myocardial function for the differentiation of pathologic and physiologic left ventricular hypertrophy: A study in athletes and...
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Báo cáo y học:

Báo cáo y học: "A comparative analysis of antibody repertoire against Staphylococcus aureus antigens in Patients with Deep-Seated versus Superficial staphylococcal Infections"

... arsenal of components and products that contribute to the pathogenesis of infections. A great deal is known about the contribution of these bacterial factors to the development of infection, [1] ... and healthy control group. These two unique bands are likely to be of diagnostic value. The diagnostic efficacy of antibody detection tests was not the primary objective of this study and these ... qualitatively by immunoblot analysis of sera from patients of both the group. Figure 2 shows the IgG immunoblot profile of the sera of patients with deep-seated and superficial infections respectively....
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Báo cáo y học:

Báo cáo y học: "ene expression analysis of human red blood cells"

... may have implications on the pathophysiology and diagnosis of various diseases involving red blood cells. Key words: red blood cell, gene expression analysis Introduction Human erythrocytes ... Interestingly, human RBCs contain 40-50% of genes encoding cell cycle processes (including 3-5% of genes for transcription/translation) as compared to only 10-20% of genes responsible for ... human erythrocyte proteome: analysis by ion trap mass spectrometry. Mollecular und Cellular Proteomics 2004;3:501-509. 9. Tyan YC, Jong SB, Liao JD et al. Proteomic profiling of eryth-rocyte proteins...
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Báo cáo Y học: A functional role of the membrane-proximal extracellular domains of the signal transducer gp130 in heterodimerization with the leukemia inhibitory factor receptor pot

Báo cáo Y học: A functional role of the membrane-proximal extracellular domains of the signal transducer gp130 in heterodimerization with the leukemia inhibitory factor receptor pot

... were regularly checked for the absence of mycoplasma infection using PCR detection of mycoplasmaDNA.Plasmid constructionConstruction of gp130 wild-type and domain mutantexpression vectors D4, ... membrane-bound gp130 proteins byflow cytometry.COS7 cells were transiently transfected using the Fugenemethod. Efficiency of transfection was analysed by flowcytometry.Flow cytometryCells were collected, ... in thedimerization and activation of gp130 by formation of disulfide bonds [30]. The analysis of cysteine residues ingp130 led to the finding that the two N-terminal cysteines of D5 (C458 and...
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Báo cáo y học:

Báo cáo y học: " Quantitative biomarker analysis of synovial gene expression by real-time PCR" potx

... osteoarthritis synovium. Based on determinations of sampling error and coefficient of variation, twofold differences in gene expression in serial biopsies can be detected by assayingapproximately six synovial ... variation in PCR assays is slightlygreater than that required for a clinical laboratory analysis [26], the precision is sufficient to allow detection of twofold differences. Analysis of individual ... [5,19]. One potential advantage of image analysis- based IHC is the ability to evaluate selec-tive regions of the synovium, although unintentional bias of Arthritis Research & Therapy Vol 5...
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