Báo cáo y học: "A Drosophila protein-interaction map centered on cell-cycle regulators" potx

... Gal4 yeast two-hybrid system [6]. We con- structed yeast arrays for use in the LexA-based two-hybrid system by subcloning approximately 12,000 Drosophila ORFs, using the same PCR amplification ... inter- action map because cell-cycle regulatory systems are known to be highly conserved in eukaryotes, and because previous results have suggested that the cell-cycle regulatory network is...

Ngày tải lên: 14/08/2014, 14:21

... normalization makes the assumption that the most frequent tag count cor- responds to nucleosomes that fully occupy their position, and is justified by the reasonable expectation that the most com- monly ... these different conventions have the potential to cause confusion and conflicting interpretations, we sought to develop a con- vention for defining a nucleosome reference map in Saccha-...

Ngày tải lên: 09/08/2014, 20:20

... Nacional Autónoma de México) by a fellowship from the Consejo Nacional de Ciencia y Tecnología (Mexico). This work was partially supported by the “Consejos de Ciencia y Tecnología Nacional ... (mediated by NAPs) in background regulation, and the digital compo- nent by the qualitatively more effective (almost binary) regulation exerted by DNA-binding specific transcription factors .....

Ngày tải lên: 09/08/2014, 20:21

... sequencing. A genetic map is a necessary companion to the sequence. In addition, because there was originally no physical map for the honey bee genome project, a meiotic map was the only resource for ... cited. Honey bee linkage map& lt;p>The meiotic map of the honey bee is presented, including the main features that emerged from comparisons with the sequence-based physical map....

Ngày tải lên: 14/08/2014, 07:21

... Senftleben U, Cao Y, Xiao G, Greten FR, Krahn G, Bonizzi G, Chen Y, Hu Y, Fong A, Sun SC, et al.: Activation by IKKalpha of a sec- ond, evolutionary conserved, NF-kappa B signaling pathway. Science ... regulator activity Transcription regulator activity Transcription factor activity DNA binding Ion transporter activity Enzyme activator activity Lipid binding (a) (b) R104.14 Genome Biology...

Ngày tải lên: 14/08/2014, 07:21

... by hybridization with individual, nonredundant ESTs to identify clones carrying single copy sequences. This approach helped confirm BAC contigs and allowed us to iden- tify functional genes on ... produce a sec- ond-generation map containing 1,755 SNP markers; con- struction of BAC contigs using two methods, namely restriction digest fingerprinting of BAC clones and hybridiza- tion of EST...

Ngày tải lên: 14/08/2014, 08:20

... interactions is shown, as well as the number of interactions identified using each detection method. In silico only are interactions only predicted by in silico methods without any confirmation from ... protein-interaction data becomes available. For this reason, we strongly encourage the continu- ation of model organism protein-interaction mapping projects. Methods of verifying protei...

Ngày tải lên: 14/08/2014, 14:21

... type, and only chromosomes from F2 progeny carrying a confirmed mutation are balanced. F1 CG31666-w + + CG32111-w + + y, w; ; x y, w CG32111-w + , CGyyy ∆ TM6B y, w; CG31666-w + , CGxxx ∆ CyO y, ... mosaic for the mutations. Given that mosaicism can only be scored in eyes and there only in nonoverlapping expression domains, the mutation rates discussed below may be slightly underesti- ma...

Ngày tải lên: 14/08/2014, 14:21

... for Psychiatric and Behavioral Genetics, Virginia Commonwealth University, Department of Psychiatry, Richmond, VA 23298-0126, USA. Correspondence: Trudy FC Mackay. Email: trudy_mackay@ncsu.edu © ... causally associ- ated with variation in aggression, due to functional polymorphisms in cis-regulatory regions, and which tran- scripts are trans-regulated and change expression as a conse- quenc...

Ngày tải lên: 14/08/2014, 21:20

... Oslo, Norway. 4 University of Oslo, Faculty Division Oslo University Hospital, Kirkeveien, Oslo, Norway. Authors’ contributions TS was involved in the study conception and design, acquisition of data ... permission to function like an emergency vehi- cle with light-and-siren response for the study purpose only. This study of documentation quality was part of a cross-sectional MVA study evalua...

Ngày tải lên: 25/10/2012, 09:56