Báo cáo y học: "A first-draft human protein-interaction map" doc

... analyzed by RT-PCR. Flow cytometry was utilized to determine caspase-3 in human cancer cell lines. Results: Viability, proliferation and apoptosis of the tissues were moderately affected by cultivation. ... regimens. Viability To visualize cell viability in slices of exemplary NSCLC tis- sue specimens, 2-photon microscopy was used in combi- nation with the LIVE/DEAD ® viability/cytotoxicit...

Ngày tải lên: 12/08/2014, 15:20

... References 1. Mayer J, Boldt J, Mengistu AM, Röhm KD, Suttner S: Goal-directed intraoperative therapy based on autocalibrated arterial pressure waveform analysis reduces hospital stay in high-risk ... Singer M: The validity of trans-esophageal Doppler ultrasonography as a measure of cardiac output in critically ill adults. Intensive Care Med 2004, 30:2060-2066. 3. Mayer J, Boldt J, Poland...

Ngày tải lên: 13/08/2014, 20:21

... Biology 2004, Volume 5, Issue 12, Article R96 Stanyon et al. http://genomebiology.com/2004/5/12/R96 Genome Biology 2004, 5:R96 Yeast two-hybrid arrays Two yeast arrays were constructed by homologous ... was gen- erated using the Gal4 yeast two-hybrid system [6]. We con- structed yeast arrays for use in the LexA-based two-hybrid system by subcloning approximately 12,000 Drosophila ORFs, using...

Ngày tải lên: 14/08/2014, 14:21

... N,N¢-diisopropylethylamine; DMS, dimethylsulfide; EDT, ethanedithiol; hCt, human calcitonin; MBHA, 4-methyl benzhydryl- amine resin; Mtt, 4-methyltrityl; Pip, 2-phenylisopropyl; OFm, fluoren-9-yl methylester; sCt, salmon ... in vivo hypocalcemic potencies were assessed. The novel analogues include cyclo17,21-[Asp17, D -Phe19, Lys21]hCt (2), cyclo17,21-[Asp17,Aib18,Lys21]hCt (3), cyclo17,21-[A...

Ngày tải lên: 31/03/2014, 21:21

... study of emotion. Psychophysiology 1996, 33:156-161. 11. Bradley MM, Cuthbert BN, Lang PJ: Startle reflex modification: emotion or attention? Psychophysiology 1990, 27:513-522. 12. Bradley MM, ... startle. Psychophysiology 1997, 34:23-31. 24. Grillon C, Davis M: Acoustic startle and anticipatory anxiety in humans: effects of monaural right and left ear stimulation. Psychophysiology 1995, 32:1...

Ngày tải lên: 09/08/2014, 01:21

... chondrocytes) shows superinduction of inflamma- tory mediators as observed by gene chip analysis, but fails to show the cardinal signs of inflammation [3]. These types of analyses will not only facilitate ... therapy and diagnosis. A system approach to arthritis ‘General System Theory’, proposed in 1940, has per- vaded all fields of science and has penetrated into popular thinking in psycholo...

Ngày tải lên: 09/08/2014, 01:21

... 0.092. Association study As reported in Table 2, the CT genotypes ( 452 His/ 452 Tyr heter- ozygosity) and the TT genotypes ( 452 Tyr/ 452 Tyr homozygosity) of the C+1354T SNP were underrepresented in SSc ... Role of 5-hydroxytryptamine in platelet thrombus formation and mechanisms of inhibition of thrombus forma- tion by 5-hydroxytryptamine 2A antagonists in rabbits. Arch Int Pharmacodyn Th...

Ngày tải lên: 09/08/2014, 13:21

... is a potential dependency among these two data types and protein-protein interologs from yeast, worm and fly since they all rely on the same phylogenetic trees. Many human protein interactions ... from human curated pathways, it is easy to superimpose pathways back to these subnetworks to see what pathways are involved in these cancer genes. Many pathways are statistically signif- icantly...

Ngày tải lên: 09/08/2014, 20:22

... SNVs were homozygous in gDNA and yet heterozygous in cDNA, and the median coverage for these SNVs in gDNA was only 12×, compared to 28× for SNVs that matched for zygosity. It is likely that many of the ... dataset, 10% of the 19,054 SNVs that overlapped by location between gDNA and cDNA were heterozygous in gDNA and yet homozygous in cDNA. Differences in zygosity between the two meth- ods can...

Ngày tải lên: 09/08/2014, 20:22

... identi- fied by PCR analysis. (b) q-PCR analysis and Luciferase assays using Dual Luciferase Reporter Assay System was performed on mES clones overex- pressing the firefly luciferase (Luc) gene. The system ... (HSP-65) 0.78 0.75 3410 Eif1a Eukaryotic translation initiation factor 1A, Y- linked 0.8 0.76 403 Pkm2 Pyruvate kinase isozyme M2 1.26 3.05 935 Bdh1 D-beta-hydroxybutyrate dehydrogena...

Ngày tải lên: 09/08/2014, 20:22