Báo cáo y học: "Global nucleosome occupancy in yeast" pps

... gain further insight into the role of nucleosomes in gene regulation, we systematically evaluated promoter nucleo- some occupancy in yeast by immunoprecipitating nucleo- somal DNA and quantifying ... of rapamycin treatment on nucleosome occupancy in the vicinity of these consensus sites. Although ribosomal protein gene expression is dramatically reduced by rapamycin [31,32], Rap1...

Ngày tải lên: 14/08/2014, 14:21

... [8] investigates nucleosome occupancy specifically at yeast promoters [9]. Both groups used a combination of chromatin immunoprecipitation and microarray analysis: after cross-linking proteins ... to determine the relative histone occupancy at the entire genome, whereas Bernstein et al. [9] used the same approach with only intergenic DNA in order to determine histone occupancy in...

Ngày tải lên: 14/08/2014, 14:21

... phosphatases play relevant roles in the biology of the yeast (cell integrity, cell c ycle regulation, cation homeostasis, etc) and that they interact with several key signalling pathways. In some cases, ... rcelona, Spain. E-mail: Joaquin.Arino@uab.es Abbreviations: MAP kinase, mitogen activated protein kinase; TOR, target of rapamycin; PP1, type 1 protein phosphatase; PP2A, type 2A prote...

Ngày tải lên: 17/03/2014, 23:20

... ml/kg hypertonic saline in hydroxyethyl starch. Following administration there was a small effect on oxygenation by increasing cardiac output and oxygen delivery. There was no control group in this study for ... sepsis are only descriptive. Furthermore, administration of hypertonic saline solutions during the early stages of septic shock when the patient is not yet hyperdynamic has not be...

Ngày tải lên: 12/08/2014, 19:21

... insertions directly into the Myc locus, almost all T lymphomas overexpress Myc as compared to normal splenocytes and thymocytes, whether there are insertions into the Myc locus, Pvt1 locus, or into an ... the nearby Myc locus – Pvt1 and the microRNAs encoded by it are as important as Myc in T lymphomagenesis, and, presumably, in T cell activation. An analysis of the co-mutations in the...

Ngày tải lên: 13/08/2014, 06:20

... poly-ubiquitin-binding protein resin and followed this with denaturing Ni-chelate chromatography in order to purify ubiquitinated proteins from yeast cells expressing 6xHis-ubiquitin. Remarkably, ... abundant topology, followed by Lys63 and Lys11 chains and the less frequent linkages through Lys33, Lys27, Lys6, and Lys29 (the latter is detected only in combination with Lys33 linkage) [42]...

Ngày tải lên: 14/08/2014, 14:22

... regeneration in this study, another recent study describes up-regulation of select inflammatory cytokines (Hmgb1 and INF -y) during post- pneumonectomy lung regeneration, although most cytokines studied ... time-dependent analysis), Ingenuity Pathway Analysis (IPA) version 2.0 (Ingenuity ® Systems Inc, Redwood City, CA; http://www.ingenuity.com ) was used to search for biological functions...

Ngày tải lên: 12/08/2014, 14:20

... Hitsuda Y, Matsumoto Y, Sasaki T: Functional assay of NF-kappaB translocation into nuclei by laser scanning cytometry: inhibitory effect by dex- amethasone or theophylline. Naunyn Schmiedebergs Arch Pharmacol ... an anti- inflammatory effect by inhibiting IL-13 production in lymphocyte and peripheral blood mononuclear cells [43,44]. In this study, we also investigated the influence of c...

Ngày tải lên: 12/08/2014, 18:22

... com- bined subtype. The predominantly inattentive subtype was diagnosed in 86 (25.4%) of the boys and 38 (45.8%) of the girls. The subgroups "predominantly hyperactive- impulsive subtype" ... obtained by using other instruments that distinguish between various times of the day, such as the Daily Parent Rating of Evening and Morning Behavior (DPREMB) scale [14] or the Weekly Rating...

Ngày tải lên: 13/08/2014, 18:21

... investigating whether these find- ings translate into optimized volume therapy in acutely ill patients are clearly warranted. Competing interests The study was supported by PULSION Medical Systems ... responsive. In contrast, if the EVLWI shows a pronounced increase, while GEDVI rises only marginally, this may be an indicator of overhydration. Limitations of the study The patients in ou...

Ngày tải lên: 13/08/2014, 20:21