Báo cáo sinh học: "Construction and characterization of a BAC library from a gynogenetic channel catfish Ictalurus punctatus" pps

... domains. The largest domain encompasses strands b1 to b6 a nd strand b7 to the C-terminus. The central b sheet is composed of 7 parallel b strands associated to an anti- parallel strand (b2) and is ... of Rv1399c from M. tuberculosis (Eur. J. Biochem. 271) 3955 Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis...

Ngày tải lên: 07/03/2014, 16:20

... viruses bearing genetic insertions between the E and NS1 genes must be stable to be useful for the development of new live attenuated vaccine viruses expressing antigens of other pathogens. The genetic ... YF 17D and 17D-Dengue 4 (YF17D/DEN4) viruses bearing the green fluorescent protein gene between E and NS1. We have characterized foreign gene expression and gen...

Ngày tải lên: 18/06/2014, 18:20

... produced from the cDNA clone. The resultant transcripts were used to transfect RD cells. At 72 h post-transfection, cells and supernatants were harvested and analyzed by microscopy and biochemical ... and testing of candidate live attenuated vaccines and antiviral therapeutics against CVA16. Methods Cells and viruses RD and Vero cells were grown in DMEM (Gibco, Grand Is...

Ngày tải lên: 18/06/2014, 18:20

... Detection and characterization of translational research in cancer and cardiovascular medicine. Journal of Translational Medicine 2011 9:57. Submit your next manuscript to BioMed Central and take ... leading journals in cancer and cardiovascular medicine. For cancer, we used JCR’s “Oncology” cate- gory. For cardiovascular medicine, we combined “Car- diac &am...

Ngày tải lên: 18/06/2014, 19:20

... R BioMed Central Page 1 of 15 (page number not for citation purposes) Virology Journal Open Access Research Development and characterization of positively selected brain-adapted SIV Peter J ... study represents a new, brain-adapted genotype of SIV with a unique phenotype of infection. Further study of this gen- otype and its unique phenotype, both alone and in com...

Ngày tải lên: 19/06/2014, 08:20

... the different domains. Herein we describe the design, construction and regenera- tion of live YF 17D and 17D-Dengue 4 (YF17D/DEN4) viruses bearing the green fluorescent protein gene between E and ... the cleavage between E and NS1 is carried out by the cellular signal peptidase present in the lumen of endoplasmic reticulum where virus maturation takes place. Therefor...

Ngày tải lên: 20/06/2014, 01:20

... first construction and characterization of an infectious cDNA clone of CVA16. The availability of this infectious clone will greatly enhance future virological investigations and vaccine development ... Tan XJ, Wang HY, Yan DM, Zhu SL, Wang DY, Ji F, Wang XJ, Gao YJ, Chen L, et al: An outbreak of hand, foot, and mouth disease associated with subgenotype C4 of...

Ngày tải lên: 20/06/2014, 01:20

... tissues and ESM from eggs collected from commercial broiler breeder farmsFigure 1 Detection of CAV DNA in pooled embryonic tissues and ESM from eggs collected from commercial broiler breeder ... we report detection of CAV and characterization of isolates based on sequence and phylogenetic analysis of partial VP1 gene from commercial broiler bre...

Ngày tải lên: 20/06/2014, 01:20

... al.: Identification and characterization of alkaline serine protease from goat skin surface metagenome. AMB Express 2011 1:3. Submit your manuscript to a journal and benefi t from: 7 Convenient ... ORIGINAL ARTICLE Open Access Identification and characterization of alkaline serine protease from goat skin surface metagenome Paul Lavanya Pushpam,...

Ngày tải lên: 21/06/2014, 05:20

... Materials from Fly Ash: A Waste from Thermal Power Stations, by High Energy Ball Milling K. Thomas Paul ặ S. K. Satpathy ặ I. Manna ặ K. K. Chakraborty ặ G. B. Nando Received: 27 April 2007 / Accepted: ... conventional materials. The key charac- teristics of nanomaterials are its small size, narrow size distribution, low levels of agglomeration and high di...

Ngày tải lên: 22/06/2014, 18:20

Ngày tải lên: 07/08/2014, 20:23

... a bovine BAC library with four genome-equivalent coverage André E GGEN a, ∗ , Mathieu G AUTIER a , Alain B ILLAUT d , Élisabeth P ETIT a , Hélène H AYES a , Pascal L AURENT a , Catherine U RBAN b , Martha ... represented in BAC libraries is around 28. Added to the bovine YAC, ovine BAC [10] and goat BAC [8] libraries hosted at Inra, this gives a 15 genome-e...

Ngày tải lên: 09/08/2014, 18:21

... ied a Haspin kinase, designated AtHaspin, in Arabidopsis thaliana. The purified AtHaspin phosphorylated histone H3 at both Thr3 and Thr11 in vitro. Live imaging of AtHaspin-tdTomato and GFP -a- tubulin in ... aurora-like kinases in Arabidopsis and other plants. Plant Cell 2005, 17(3):836-848. 11. Kawabe A, Matsunaga S, Nakagawa K, Kurihara D, Yoneda A, Hasezawa S, Uchiyam...

Ngày tải lên: 11/08/2014, 11:22

... isolates of DEV will also be of importance in order to assess the possible role of the UL31 protein in viral pathogenesis. These properties of the UL31 protein provide a prerequisite for further ... analysis of coding sequences of UL31 predicts a 35.7 kDa basic protein, and thus the molecular mass of the protein reacted with the UL31 antiserum was co...

Ngày tải lên: 12/08/2014, 04:21

... 10.1051/gse:2003046 Original article Construction and characterization of a BAC library from a gynogenetic channel catfish Ictalurus punctatus Sylvie M .A. Q UINIOU a, b , Takayuki K ATAGIRI a , Norman W. M ILLER a , ... 571–576. [13] Katagiri T., Asakawa S., Minagawa S., Shimizu N., Hirono I., Aoki T., Construc- tion and characterization of BAC libraries f...

Ngày tải lên: 14/08/2014, 13:22