... 1Division of Human Gene Therapy, Department of Medicine, University of Alabama at Birmingham, Birmingham, USA, 2Division of Human Gene Therapy, Departments of Pathology, University of Alabama ... 3Division of Human Gene Therapy, Departments of Surgery, University of Alabama at Birmingham, Birmingham, USA, 4Division of Human Gene Therapy, Departments of Cell Biology, University of Alabama ... L-glutamine. All of the cells weremaintained at 37°C in a 5% CO2 humidified incubator.Generation of the Ad5.PTDtat vector Genetic modification of the Ad5 vector with PTDtat wasachieved using our...