analysis of genes and genomes phần 10 pptx

... immunoprecipi- tation and CpG island microarray analysis. Genes Dev., 16, 235–244. Weiss, B. and Richardson, C.C. (1967) Enzymatic breakage and joining of deoxyri- bonucleic acid, I. Repair of single-strand breaks ... Cell, 107 , 415–418. Norrander, J., Kempe, T. and Messing, J. (1983) Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis. Gene...

Ngày tải lên: 14/08/2014, 11:21

... suggested by Watson and Crick. The separation of the DNA strands of the parent helix (black) means that each strand can act as a template of the synthesis of a new strand of DNA (blue). This ... Meselson and Stahl, however, dismissed this mode of replication by the analysis of individual single strands of replicated DNA. They heated their DNA (Figure 1.11) and found th...

Ngày tải lên: 14/08/2014, 11:21

... vitro mutagenesis 10 20 pBluescript YAC Construction of genomic libraries 100 0–2000 pYAC4 PAC Construction of genomic libraries 75–90 pAd10SacBII BAC Construction of genomic libraries 130–150 pBAC108L • ... functionally inactivated by the presence of the insert DNA 108 BASIC TECHNIQUES IN GENE ANALYSIS 2 bacterial cells (1–5 mL of bacterial culture) and can yield up to 10...

Ngày tải lên: 14/08/2014, 11:21

... between 17 and 30 nucleotides in length, which flank the DNA target sequence that is to be copied. One of the primers is the same sequence as one strand of the DNA Analysis of Genes and Genomes Richard ... ends of the + strand are ligated to form the single-stranded genome. The switch between the double-stranded RF form and the single-stranded + form of the M13 viral geno...

Ngày tải lên: 14/08/2014, 11:21

... a biochemical function of the polypeptide or • the ability of the polypeptide encoded by the recombinant clone to interact with other polypeptides. Analysis of Genes and Genomes Richard J. Reece  ... bait and prey, in yeast (Fields and Song, 1989). The screen was performed in a yeast strain that was deleted for the wild-type copies of the GAL4 and GAL80 genes, and th...

Ngày tải lên: 14/08/2014, 11:21

... base alteration per 10 6 10 8 bases replicated) and varies widely between individual genes and organisms. Naturally occurring mutations have, however, been used to isolate genes and describe specific ... 7.2). Procedures 0 102 03040506070809 0100 Efficiency of mutation (%) 0 5 10 15 20 Number of clones analysed to give 90% chance of isolating the mutant Figure 7.2. Effic...

Ngày tải lên: 14/08/2014, 11:21

... of changes in the expression of all of the thousands or tens of thousands of genes within a genome is essential if we are to understand the interplay between genes and gene products. DNA microarrays ... in expression of genes within the tissue samples can be detected through the analysis of the intensity and individual bands. (b) Differential display of four RNA sa...

Ngày tải lên: 14/08/2014, 11:21

... between five and 10 chloroplast genomes. Therefore, each plant cell may contain > ;100 00 chloroplast genomes. Transformation is likely to result in the alteration of just one of these genomes. A ... rounds of regeneration and selection Figure 11.6. Selection of homogenous transgenic chloroplasts. Plant cells can contain between 50 and 100 chloroplasts, and each of th...

Ngày tải lên: 14/08/2014, 11:21

... contributions to the establishment of oligonucleotide based, site directed mutagenesis and its development for protein studies (Chemistry) Analysis of Genes and Genomes Richard J. Reece  2004 John ... mitosis and meiosis Chromatid – one of the usually paired and parallel strands of a duplicated chromosome joined by a single centromere Chromatin – a complex of DNA and...

Ngày tải lên: 14/08/2014, 11:21