analysis of genes and genomes phần 10 pptx
analysis of genes and genomes phần 10 pptx
... immunoprecipi-
tation and CpG island microarray analysis. Genes Dev., 16, 235–244.
Weiss, B. and Richardson, C.C. (1967) Enzymatic breakage and joining of deoxyri-
bonucleic acid, I. Repair of single-strand breaks ... Cell, 107 , 415–418.
Norrander, J., Kempe, T. and Messing, J. (1983) Construction of improved M13
vectors using oligodeoxynucleotide-directed mutagenesis. Gene...
analysis of genes and genomes phần 2 doc
... suggested by Watson and
Crick. The separation of the DNA strands of the parent helix (black) means that each
strand can act as a template of the synthesis of a new strand of DNA (blue). This ... Meselson and Stahl,
however, dismissed this mode of replication by the analysis of individual single
strands of replicated DNA. They heated their DNA (Figure 1.11) and found
th...
analysis of genes and genomes phần 3 pot
... vitro
mutagenesis
10 20 pBluescript
YAC Construction of genomic libraries 100 0–2000 pYAC4
PAC Construction of genomic libraries 75–90 pAd10SacBII
BAC Construction of genomic libraries 130–150 pBAC108L
• ... functionally inactivated by the presence of
the insert DNA
108 BASIC TECHNIQUES IN GENE ANALYSIS 2
bacterial cells (1–5 mL of bacterial culture) and can yield up to 10...
analysis of genes and genomes phần 4 potx
... between 17 and 30
nucleotides in length, which flank the DNA target sequence that is to be
copied. One of the primers is the same sequence as one strand of the DNA
Analysis of Genes and Genomes Richard ... ends of the + strand are ligated
to form the single-stranded genome.
The switch between the double-stranded RF form and the single-stranded +
form of the M13 viral geno...
analysis of genes and genomes phần 5 potx
... a biochemical function of the polypeptide or
• the ability of the polypeptide encoded by the recombinant clone to interact
with other polypeptides.
Analysis of Genes and Genomes Richard J. Reece
... bait and prey,
in yeast (Fields and Song, 1989). The screen was performed in a yeast strain
that was deleted for the wild-type copies of the GAL4 and GAL80 genes, and
th...
analysis of genes and genomes phần 6 pps
... base alteration per 10
6
10
8
bases replicated) and varies widely between individual genes and organisms.
Naturally occurring mutations have, however, been used to isolate genes and
describe specific ... 7.2). Procedures
0 102 03040506070809 0100
Efficiency of mutation (%)
0
5
10
15
20
Number of clones analysed to give
90% chance of isolating the mutant
Figure 7.2. Effic...
analysis of genes and genomes phần 7 pot
... of changes in the expression of all of the thousands or tens of
thousands of genes within a genome is essential if we are to understand the
interplay between genes and gene products.
DNA microarrays ... in
expression of genes within the tissue samples can be detected through the analysis of the
intensity and individual bands. (b) Differential display of four RNA sa...
analysis of genes and genomes phần 8 pdf
... between five and 10 chloroplast genomes. Therefore, each plant cell may
contain > ;100 00 chloroplast genomes. Transformation is likely to result in the alteration of
just one of these genomes. A ... rounds
of regeneration
and selection
Figure 11.6. Selection of homogenous transgenic chloroplasts. Plant cells can contain
between 50 and 100 chloroplasts, and each of th...
analysis of genes and genomes phần 9 ppsx
... contributions to the establishment of
oligonucleotide based, site directed mutagenesis and its development for protein
studies (Chemistry)
Analysis of Genes and Genomes Richard J. Reece
2004 John ... mitosis and meiosis
Chromatid – one of the usually paired and parallel strands of a duplicated chromosome
joined by a single centromere
Chromatin – a complex of DNA and...
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