analysis of genes and genomes phần 8 pdf

... be induced to reproduce Analysis of Genes and Genomes Richard J. Reece  2004 John Wiley & Sons, Ltd ISBNs: 0-470 -84 379-9 (HB); 0-470 -84 380 -2 (PB) 3 28 POST-GENOME ANALYSIS 10 Gene 1 URA3 URA3 Ura + PCR ... manipulate using standard techniques. However, the isolation of a full-length cDNA clone corresponding to entire RNA genomes (Ahlquist and Janda, 1 984 ) has pe...

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... suggested by Watson and Crick. The separation of the DNA strands of the parent helix (black) means that each strand can act as a template of the synthesis of a new strand of DNA (blue). This ... biology. The discovery of enzymes able to cleave DNA at specific Analysis of Genes and Genomes Richard J. Reece  2004 John Wiley & Sons, Ltd ISBNs: 0-470 -84 379-9 (HB); 0-...

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... Construction of genomic libraries ∼23 λZAP, EMBL4 Cosmid Construction of genomic libraries ∼44 pJB8 M13 In vitro mutagenesis DNA sequencing 8 9 M13mp 18 Phagemid General DNA manipulation In vitro mutagenesis 10–20 ... 2.13). 10000 Size (bp) Distance migrated (mm) 80 00 6000 5000 4000 3500 3000 2500 2000 1500 1000 750 500 250 10000 12.00 80 00 12 .85 6000 14.55 5000 16.12 4000 18. 24...

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... between 17 and 30 nucleotides in length, which flank the DNA target sequence that is to be copied. One of the primers is the same sequence as one strand of the DNA Analysis of Genes and Genomes Richard ... 3 product of gene 2. Following cleavage, the two ends of the + strand are ligated to form the single-stranded genome. The switch between the double-stranded RF form and...

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... This region of the protein interacts directly with DNA at a specific sequence called 1 100 200 300 400 500 600 700 80 0 88 1 1 65 65 94 85 1 88 1 88 17 681 961 48 DNA recognition Dimerization Gal80p binding DNA Binding Transcription activation Gal4p Figure ... bait and prey, in yeast (Fields and Song, 1 989 ). The screen was performed in a yeast strain that was deleted for the wild...

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... architecture of a typical expression vector is shown in Figure 8. 1. Analysis of Genes and Genomes Richard J. Reece  2004 John Wiley & Sons, Ltd ISBNs: 0-470 -84 379-9 (HB); 0-470 -84 380 -2 (PB) 254 ... basis of both charge and size, reveals a large number of spots corresponding to individual proteins (Figure 8. 8). This analysis shows that many proteins are of a...

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... function. The analysis of changes in the expression of all of the thousands or tens of thousands of genes within a genome is essential if we are to understand the interplay between genes and gene ... may be translated at Analysis of Genes and Genomes Richard J. Reece  2004 John Wiley & Sons, Ltd ISBNs: 0-470 -84 379-9 (HB); 0-470 -84 380 -2 (PB) 8. 5 PROTEIN...

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... G. and Brown, R. (1 985 ) Use of gene transfer and a novel cosmid rescue strategy to isolate transforming sequences. EMBO J., 4, 2 583 –2 588 . Brandhorst, B.P. and McConkey, E.H. (1974) Stability of ... the fertilized Analysis of Genes and Genomes Richard J. Reece  2004 John Wiley & Sons, Ltd ISBNs: 0-470 -84 379-9 (HB); 0-470 -84 380 -2 (PB) 404 GLOSSARY Homology –...

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... Proc.Natl.Acad.Sci.USA, 83 , 5414–54 18. Wyckoff, E. and Hsieh, T.S. (1 988 ) Functional expression of a Drosophila gene in yeast: genetic complementation of DNA topoisomerase II. Proc. Natl. Acad. Sci. USA, 85 , 6272–6276. Wysocki, ... Nature, 349, 785 – 788 . Lawyer, F.C., Stoffel, S., Saiki, R.K., Myambo, K., Drummond, R. and Gelfand, D.H. (1 989 ) Isolation, characterization,...

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