Báo cáo y học: " Caenorhabditis elegans comes of age" pptx

... that quite a bit of the work described above was already envisaged by Sydney Brenner in the early 1960s, before both the authors of this report were born. http://genomebiology.com/2008/9/6/312 Genome BBiioollooggyy 2008, ... and the research community worldwide, approximately one-quarter (around 5,000) of all C. elegans genes has been knocked out, all of which are publicly available...

Ngày tải lên: 14/08/2014, 08:21

... by LaDeana Hillier (W ashington University in St Louis) and Robert Waterston (University of Washington). C. elegans strains were p rovided by the Caenorhabditis Genetics Center (University of ... undetectable in early embryos as those with RNA-seq dcpm (depth of coverage per base per million reads) equals 0 in early embryos. E Emb, early embryo; L Emb, late embryo; L, larva stage; A...

Ngày tải lên: 09/08/2014, 22:23

... by microarrays or serial analysis of gene expression (SAGE), respectively, to tackle this problem. Fox reported on the profiling of cells from the embryonic motor circuit, where she not only ... the beginning of a extensive series of yeast two-hybrid experi- ments at Harvard, which will use this ORFeome resource to build a complete map of interactions among the proteins expressed by...

Ngày tải lên: 14/08/2014, 15:20

... absence of what they call ‘furnishings’ - you know, that little moustache and bushy eyebrows you have. Clifford (proudly): I am well furnished. Mink: Of course you are. Well, after they looked ... with little mounds of - Mink: Yes, yes, I know. I can’t help it. But as I was saying, I have solid brown, straight fur while you have patchy off- white and beige curly hair. Your coat would...

Ngày tải lên: 09/08/2014, 20:20

... ffoorr ssttuuddyyiinngg ddiiaattoomm bbiioollooggyy iinn PPhhaaeeooddaaccttyylluumm ttrriiccoorrnnuuttuumm Gene 2007, 440066:: 23-25. http://genomebiology.com/2008/9/12/245 Genome BBiioollooggyy 2008, ... nitrogen cycling in diatoms: a catabolic urea cycle has been identified involving ornithine and citruline and potentially yielding urea and subsequently ammonia from hydrolysis of the s...

Ngày tải lên: 14/08/2014, 21:20

... Fortney et al., Inferring the functions of longevity genes with modular subnetwork biomarkers of Caenorhabditis elegans aging Genome Biology 2010, 11:R13 Fortney et al. Genome Biology 2010, ... separately for two different C. elegans aging microarray datasets: 104 microarrays of individual wild-type (N2) worms over 7 ages (9 to 17 microarrays per age) [2], and 16 microarrays of...

Ngày tải lên: 09/08/2014, 20:21

... embryonic lethality was scored for the homozygous progeny of homozygous parents. For strains carrying 100% lethal alleles, embryonic lethality was scored for the mixed progeny of either heterozygous ... soaked C. elegans. For statistical analysis of the data, percent embryonic lethality was converted to survival (1 - % embryonic lethality). All survival values were normalized by the s...

Ngày tải lên: 14/08/2014, 14:21

... Carbethoxylation of coordinated histidine by diethylpyrocarbonate. J Inorg Biochem 51, 727–735. 30 Miles EW (1977) Modification of histidyl residues in proteins by diethylpyrocarbonate. Methods Enzymol 47, ... superfamily of small cysteine- rich, metal-binding polypeptides, present in all Eukaryota [2] and as also reported in Eubacteria [3,4]. They most likely evolved through a web of...

Ngày tải lên: 07/03/2014, 02:20

... higher the purity, but this comes at the expense of loss of valuable material. Organelle proteomics of this type also isolates the organelle from its cellular context, and at best can only provide ... most likely to be missed by such approaches. In the recent study in Cell by Foster et al. [6], Mann and his group have sought to avoid some of these problems by using protein correlati...

Ngày tải lên: 14/08/2014, 16:21

... ampli- fied cryptate emission (TRACE) technology assay (PCT Kryptor ® , B.R.A.H.M.S. AG, Hennigsdorf, Germany). The assay has a detection limit of 0.02 μg/L and functional assay sensitivity of 0.06 ... standardized care of patients as defined previously [34]. Thereby, LRTI was defined by the presence of at least one respiratory symp- tom (cough, sputum production, dyspnea, tachypnea,...

Ngày tải lên: 25/10/2012, 10:02