Báo cáo y học: " Gene expression profiling of human prostate cancer stem cells reveals a pro-inflammatory phenotype and the importance of extracellular matrix interactions" pps

... were dominated by the signaling of inflammatory cytokines through the JAK-STAT (Janus activated kinase-signal trans- ducer and activator of transcription) pathway and the interac- tion of cell surface ... same system, as the focal adhesion pathway is activated by extracellular matrix- receptor interaction. Changes in extracellular matrix and associated proteins have...

Ngày tải lên: 14/08/2014, 08:21

... results obtained with RNA from normal paws of naive BALB/c SCID mice (AN), clinically normal pre-arthritic paws (PA), acutely arthritic paws (AA) and chronically inflamed paws (CA). Available online ... progression and effi- cacy of treatment, or to predicting the prognosis of the dis- ease. The major obstacles in the human studies are the relatively late diagnosis...

Ngày tải lên: 09/08/2014, 06:22

... 5'-ggtcatggagatgtc- cgtaa-3' and 3'-accaatagagagaccaggaagaa-5'; GTSE1: 5'- acgtgaacatggatgacccta-3' and 3'-gttcgggaaccggattattta-3'; CDC2: 5'-ggtcaagtggtagccatgaaa-3' ... 5'-ggtcaagtggtagccatgaaa-3' and 3'-ccaggagggata- gaatccaag-5'; and MKI67: 5'-ccccaaccaaaagaaagtctc-3' and 3'-gactaggagctggagggctt...

Ngày tải lên: 09/08/2014, 14:20

... endothelial cells RNA extraction. She also provided the statistical analysis. MD and PA performed the gene array analysis. RA helped in the flow-cytometric analysis of endothelial cells. LC and PA were responsible ... K, Imai H, Harada M, Osanai H, Kondo T, Murohara T: The impact of the capability of circulating progenitor cell to differentiate on myocardial salvage in...

Ngày tải lên: 12/08/2014, 14:22

... chain reaction; SAM, statistical analysis of microarrays; TCA, tricarboxylic acid. Table 8 Sequences of the primers used for validation of microarray results by real-time PCR Gene GeneBank accession Forward ... AF_503444 5'-AAG CAG GGC AGA CAT TGC GAT-3' 5'-CAG GAT GTG GCT CTA GGC TAT GT-3' 1.98 Hamp2 AY_232841 5'-AAG CAG GGC AGA CAT TGC GAT-3' 5'-...

Ngày tải lên: 14/08/2014, 08:20

... for- ward AAC AAG AAC TAC GCA ATG GGG and reverse GTC CTC TGT TCA TAG CCT TCT G; nuclear receptor co-repressor 2 (NCOR2): forward CAG CAG CGC ATC AAG TTC AT and reverse GTA ATA GAG GAC GCA CTC AGC; ... fibrosis. A combination of the Affymetrix change algorithm (Microarray Suite 5) and of analysis of variance models was used to identify TGFβ-regulated genes. Additional criteria...

Ngày tải lên: 12/08/2014, 18:21

... articular cartilage and has been shown to play a role in collagen cleavage in at least a subset of OA patients [5]. Cathepsin O and cathepsin Z are also highly expressed genes in cartilage and, again, ... Osteoarthritis Cartilage 2009, 17:547-555. 45. Nakayama Y, Nara N, Kawakita Y, Takeshima Y, Arakawa M, Katoh M, Morita S, Iwatsuki K, Tanaka K, Okamoto S, Kitamura T, Seki N, M...

Ngày tải lên: 09/08/2014, 14:22

... instruc- tions of the manufacturer, using commercially available assays-on-demand (Applied Biosystems, Applera Deut- schland GmbH, Darmstadt, Germany). Ct values were cal- culated by the ABI PRISM software, ... statistical analysis The quality control, normalization and data analysis, were assured with the affy package of functions of statistical scripting language 'R&apo...

Ngày tải lên: 18/06/2014, 15:20

... transcription system. The recombinant RNA was quantified by the measurement of A 260 and used as a standard (after cDNA synthesis) in the quantitative RT–PCR reaction. Quantitative PCR analysis For quantification, ... Toivanen P: Expression of the myc-family proto-oncogenes and related genes max and mad in synovial tissue. Scand J Rheumatol 1999, 28:314-318. 37. Hashir...

Ngày tải lên: 09/08/2014, 01:22

... the overlying soft tissues, the patella tendon was transversally cut and the prox- imal and distal part carefully mobilized. After exposure, the 'Hoffa's fatty body' was superfused ... Feldmann M, Brennan FM, Williams RO, Woody JN, Maini RN: The transfer of a laboratory based hypothesis to a clinically useful therapy: the development of anti-TNF therapy...

Ngày tải lên: 09/08/2014, 06:23