Báo cáo y học: "Systematic analysis of transcribed loci in ENCODE regions using RACE sequencing reveals extensive transcription in the human genome" pptx

... have systematically analyzed the transcribed loci in 420 selected portions of the ENCyclopedia Of DNA Elements (ENCODE) regions using 5' -RACE and 3' -RACE sequencing. The ENCODE regions ... Biology 2008, 9:R3 Open Access 2008Wuet al.Volume 9, Issue 1, Article R3 Research Systematic analysis of transcribed loci in ENCODE regions using RAC...

Ngày tải lên: 14/08/2014, 08:20

... suggesting that if the instance compound targets the instance protein, inhibiting the heterozygous deletion strain, then co- inhibiting compounds would be more likely to inhibit the strain as ... absence of the target in yeast. In the homozygous data, several drugs with no obvious structural similarity clustered together (Figure 2c): rapamycin, calyculin A and wiskostatin....

Ngày tải lên: 09/08/2014, 20:21

... down-regulation in the autopsy samples. Interestingly, we also see up-regulation of genes involved in the ubiquitin cycle, protein ubiquitination, and ubiquitin-dependent protein catabolism. This ... expression patterns in postmortem human brains. Study of expression levels of 14 genes in human brain autopsy and biopsy samples found significant change in one of the...

Ngày tải lên: 14/08/2014, 16:20

... results in the formation of the branching epithelial tree of the adult gland. The onset of puberty results not only in the rapid growth of a ductal epithelial tree but also the appear- ance of specialized, ... list). Four out of five of the most highly upregulated transcripts through the onset of puberty are members of the MUP family of odorant-bindin...

Ngày tải lên: 14/08/2014, 14:21

... to identify key molecules in the course of hy- poxia-induced apoptotic pathways. Such analysis of trophoblastic cells will, in the near future, yield in- sights into the mechanisms of preeclampsia ... proteins induced by hypoxia in trophoblastic cells so as to clarify the mechanism of hypoxia-induced apoptosis by using the PoweBlot, an antibody-based Western a...

Ngày tải lên: 31/10/2012, 15:12

... Biochemistry of the Charité, Berlin, Germany using the ABI PRISM Big Dye Terminator system (Applied Biosystems, Germany). Sequences were analyzed using the Chromas software (Technely- sium Pty Ltd, ... confirmed by sequencing DNA samples of 51 healthy individuals. Interestingly, this SNP had not yet been registered in any SNP databases despite of its high frequency of...

Ngày tải lên: 31/10/2012, 16:49

... immunoglobulin chains were also found to be significantly increased in aged thymocytes. Understanding the changes in gene expression in thymocytes with age may hold the key in determining thymocyte ... levels of many of these genes identified in the array analysis. Moreover, Western blot analysis of thymo- cyte lysates also confirmed that IgM increases with age (...

Ngày tải lên: 03/11/2012, 11:35

... DM64 binds to the myotoxins through a myotoxic/ cytolytic site distinct from the catalytic site, as already described for the inhibition of myotoxicity by heparin [44]. At least in the case of B. ... sequences. The conserved aromatic residues phenylalanine and tyrosine typical of the Ig-fold are bold in each domain. Fig. 5. Inhibition of in vivo myotoxicity of myot...

Ngày tải lên: 21/02/2014, 01:21

... protein in the gel lane from which the density measurement was made. Centrifugation of p26 in sucrose gradients Sucrose gradients were formed in 0.1 M Tris/glycine (pH 7.4) by layering 5 mL of ... signifi- canceofasmallheatshock/a-crystallin protein (p26) in encysted embryos of the brine s hrimp, Artemia franciscana. Am. Zool. 39, 836–847. 49. Liang, P. & MacRae, T.H. (...

Ngày tải lên: 22/02/2014, 04:20

... in ammogenic activity of the intact molecule [6]. The presence of longer (compared to the highly endotoxic lipid of E. coli)chain fatty acids, the absence of phosphates and of an acyl group at O-3 of a-GlcN ... (C) p.p.m. can only be explained as resulting from the acylation of A3withacyl 3 . O-Acylation of GlcN A at O-3 and of acyl 2 at O-3 agreed with the low-...

Ngày tải lên: 08/03/2014, 09:20