Báo cáo y học: " Toward more effective gene delivery" docx

... 99:: 301 adenovirus with reactive multivalent polymers based on poly[N-(2-hydroxypropyl)methacrylamide] decreased both infection of hepatocytes and virus phagocytosis by macro- phages, increasing the percentage ... administered intravenously, face an array of challenges from the innate and adaptive immune systems, which rapidly diminish the availability of vectors for delivery to target site...

Ngày tải lên: 14/08/2014, 08:20

... maternally inherited mRNA by cytoplasmic polyadenylation in Xenopus oocytes. Polyadenylation is required before the mRNA can be trans- lated, and regulation of this step is therefore necessary for oocyte ... necessary for oocyte maturation and embryonic development. Cytoplas- mic polyadenylation depends on phosphorylation of CPEB, a protein that is bound to specific cytoplasmic polyadenylatio...

Ngày tải lên: 14/08/2014, 14:21

... and hybrid- ized to the DNA microarray in the Affymetrix Fluidics Station 400. Affymetrix human U133 X3P arrays were used. The GCOS Affymetrix GeneChip Operating System (version 1.2, Affymetrix) ... Miyamoto Y, Fukuda A, Mabuchi A, Kotani A, Kawakami A, Yamamoto S, Uchida A, Nakamura K, Notoya K, Nakamura Y, Ikegawa S: An aspartic acid repeat poly- morphism in asporin inhibits chondrogenesi...

Ngày tải lên: 09/08/2014, 14:20

... open question e.g. ‘How may I help you?’ 2. Ask the value for any one of slots 1 n. 3. Explicitly confirm any one of slots 1 n. 4. Ask for the n th slot whilst implicitly confirm- ing 1 either slot ... interpreted by the dialogue man- ager. Post-mapping user responses are made up of one or more of the following types of utterance: (1) Stay quiet, (2) Provide 1 or more slot values, (3) Yes,...

Ngày tải lên: 17/03/2014, 04:20

... 2.7.1.1); glyceraldehyde 3-phosphate dehydrogenase (EC 1.2.1.12); enolase (EC 4.2.1.11); alcohol dehydrogenase (EC 1.1.1.1); pyruvate decarboxylase isozyme 1, 2, and 3 (EC 4.1.1.1); aldehyde dehydrogenase ... Germany; 2 Humboldt University Berlin, Institute of Biology, Theoretical Biophysics, Berlin, Germany; 3 Humboldt University Berlin, Medical Faculty (Charite ´ ), Institute of Biochemistr...

Ngày tải lên: 17/03/2014, 10:20

... selection of effective AS-ODNs are based on cell-free assays, e.g. RNAse H digestion screens and oligonucleotide scanning arrays [9]. As activity in cell-free assay may not always correlate with activity ... by combinations of CYP1A1 and GSTM1 gene polymorphisms in a Japanese population. Carcinogenesis 16, 2331–2336. 17. Kawajiri, K., Watanabe, J., Eguchi, H. & Hayashi, S. (1995) Gene...

Ngày tải lên: 31/03/2014, 15:20

... microarray system used was an Affymetrix U95A GeneChip (Affymetrix, Santa Clara, CA); 4566 genes were analyzed. Four healthy donors of PBMC were studied. † The microarray system used was an Affymetrix ... U95A GeneChip; 4566 genes were analyzed. The donors of PBMC studied were 48 with SLE and 42 healthy. ‡ The microarray system used was an Affymetrix U95AV2 GeneChip; about 4600 genes were a...

Ngày tải lên: 09/08/2014, 01:23

... oxide synthase. Adenylate cyclase, 3.4 6.4 1.9 Adenylate cyclase (type 7), an ATP-pyrophosphate lyase; type VII converts ATP to cAMP C. Genes expressed in active untreated patients only Gene bank ... uniquely expressed genes. Selection of hypervariable (HV) genes To have an opportunity to evaluate inhomogeneity in gene expression variability, it is necessary to normalize this vari- abilit...

Ngày tải lên: 09/08/2014, 01:23

... X-linked genes indicate that oligoclonal or monoclonal expansion of synoviocytes can occur in chronically inflamed rheumatoid synovial tissue, especially at sites of erosions [19]. More recently, we ... arthritis (RA) fibroblast-like synoviocytes and synovial tissue samples Patient Fibroblast-like synoviocytes Synovial tissue Mutation Frequency Mutation Frequency RA10 Codon 119 GCC>ACC (...

Ngày tải lên: 09/08/2014, 06:22

... pas- sively induced by injecting 3 µg lysozyme coupled to poly- L-lysine in 6 µl pyrogen-free saline into the knee joints. Histology of arthritic knee joints Total knee joints were dissected at days ... chondro- cyte death during ICA and arthritis induced by collagen type II [10,11]. IL-4 is regarded as a potent anti-inflamma- tory cytokine by direct inhibition of proinflammatory cytokines su...

Ngày tải lên: 09/08/2014, 06:22