Báo cáo y học: "Direct selection and phage display of a Gram-positive secretom" ppsx

... (5'-CCGGAAACGTCACCAATGAA) and BigDye ® Terminator v3.1 Cycle Sequencing Kit (Applied Bio- systems, Foster City, California, USA) and was analyzed on a ABI3730 Genetic Analyzer (Applied Biosystems) at ... Haertle T, Topisirovic L: Molecular characterization of a novel bacteri- ocin and an unusually large aggregation factor of Lactobacil- lus paracasei subsp. paracasei BGSJ2...

Ngày tải lên: 14/08/2014, 08:20

... to an apparent molecular mass of 120 kDa. Protein was concentrated by ultrafiltration and analyzed by SDS/PAGE. Determination of enzyme activities The assays were routinely carried out under anoxic ... Fe-containing catalytic subunit of CO dehydrogenase. A catalytically active CooSI dimer has been previously purified and characterized from C. hydrogenoformans [25]. The 89- and 62-...

Ngày tải lên: 23/03/2014, 21:20

... cytoplasmic fraction (20 lg, lane 1) and of CCT purified from rabbit reticulocyte lysate (2 lgand6lg in lanes 2 and 3, respectively). Lanes 1 and 2 were Western blotted and immunostained by an ... CCT were quantitated by the use of a phosphori- mager and expressed as a fraction of the maximum amount of bound labeled tubulin and plotted as a function of the ratio of...

Ngày tải lên: 23/03/2014, 21:20

... hypotheses. Disposing of dying cell: a fine balance between proinflammatory and anti-inflammatory signals Phagocytosis of apoptotic cells is known to be an anti- inflammatory [15] and immunologically silent ... process of cell suicide that leads to ordered destruction of the cells and their safe disposal by professional (macrophages and immature dendritic cells) and nonpr...

Ngày tải lên: 09/08/2014, 01:23

... the analysis (Figure 1). All the study subjects were male, and the median age of the 198 patients with available age was 30 years (range, 20 to 63 years). Of these, 134 patients were new cases and the ... of one sputum sample is homogeneous and representative of the total bacillary population in a patient. Methods: We systematically analysed several pre-treatment isolates from...

Ngày tải lên: 12/08/2014, 16:20

... ion spray voltage, -4.5 kV; sheath gas (N 2 ) at a flow rate of 60 arbitrary units; auxiliary gas (N 2 ) at a flow rate of 20 arbitrary units; capillary tem- perature, 350°C; capillary voltage , ... RESEARCH Open Access Chemical fingerprinting and quantitative analysis of a Panax notoginseng preparation using HPLC-UV and HPLC-MS Hong Yao, Peiying Shi, Qing Shao, Xiaohui Fan *...

Ngày tải lên: 13/08/2014, 14:20

... such as Asia, Africa, Pacific Islands and the Arctic and the rate of HBsAg positivity ranges from 8% to 15%. In the low endemic area, such as Western countries, HBV is predominantly a disease of ... Africa. Genotype B and C are common in Asia; genotype D, in southern Europe, the Middle East, and India; genotype E, in West Africa and South Africa; genotype F, in South and...

Ngày tải lên: 02/11/2012, 11:17

... Publisher. All rights reserved Short Communication Human toxoplasmosis and the role of veterinary clinicians Laura Kramer Department of Animal Health- Veterinary Parasitology Laboratory- University ... animal clini- cians. Veterinarians are often faced with the need to explain to their clients that it is not necessary, for example, to give away one’s cat during pregnancy and tha...

Ngày tải lên: 03/11/2012, 11:06

Ngày tải lên: 03/11/2012, 11:11

... promoter TGA AGC TGG AAA CCA TCA TTC AAA ACA TTA GCA GGA ATT TTC 52 343 Lower promoter GGA GTT CTG CCA GGG AAC CAC GAC AGG GGA GAA CGC CAC TTA 57 587 Exon 1 GTG CTG CCT GAG AAG GAT TG GAA AGT GCC ... exactly with the mobilities of di-, mono- and unglycosylated forms of recombinant cystatin F previously characterized by enzymatic removal of N-linked carbohydrate and carbohydrate comp...

Ngày tải lên: 21/02/2014, 01:21