Báo cáo y học: "Gene prediction: compare and CONTRAST" pptx

... prediction over time. The gene sensitivity and specificity and the exon sensitivity and specificity on the EGASP test set [5] are shown for several programs by year of initial publication. Included ... sequences. By comparison, the accuracy of the Ensembl evidence-based gene predictions used in the EGASP experiment at the gene level were 71.6% sensitivity and 67.3% specificity and 7...

Ngày tải lên: 14/08/2014, 08:20

... was recently shown to bind the P-selectin glycoprotein ligand 1 – a molecule that plays a role in homing of T lymphocytes [32]. The role played by the cytokine receptor IL-13 receptor α 1 in arthritis ... postcapillary synovial venulesLeucocytes rolling along the endothelium of postcapillary synovial venules. Shown are the proportions of leucocytes rolling along the endothelium of postcapi...

Ngày tải lên: 09/08/2014, 06:23

... extracted from parotid, submaxillary gland, salivary glands, trachea, stomach, liver and gallbladder, duodenum and intestine and kidney by using guanidine hydrochloride as previously described [13]. RT-PCR ... map, subclone and sequence; (c) 5¢ and 3¢ ends are usually very similar between genes from the same class; and (d) clones may show instability presumably due to the repetiti...

Ngày tải lên: 08/03/2014, 23:20

... were collected, and live cells (lymphocytes) were separated on Lympholyte-M (Cedarlane, Ontario, Canada). Finally, 2 × 10 7 lymphocytes were injected intraperitoneally on days 0 and 7 into recipient ... Proteoglycan-induced polyarthritis and spondylitis adoptively transferred to naive (nonimmunized) BALB/c mice. Arthritis Rheum 1990, 33:866-876. 31. Mikecz K, Glant TT: Migration and...

Ngày tải lên: 09/08/2014, 06:22

... study, we analyzed gene-expression profiles in the syn- ovial lining tissues in situ in early RA using synovial specimens obtained by targeted biopsy, followed by LCM and cDNA microarray analyses. ... in early RA. That is, we prepared synovial specimens from early RA by targeted biopsy under arthros- copy, and analyzed gene-expression profiles in the synovial lin- ing tissues selected by...

Ngày tải lên: 09/08/2014, 06:23

... 4 Protein levels and collagenolytic activity more strongly inhibited by dual treatment with LG268 and rosiglitazoneProtein levels and collagenolytic activity more strongly inhibited by dual treatment ... a polyclonal MMP-13 antibody generously provided by Peter Mitchell (Pfizer Inc, New York, NY, USA). Protein bands were visualized by incubation with a goat anti-rabbit secondary antibod...

Ngày tải lên: 09/08/2014, 13:22

... cartilage-degrading glycosidases was moderately downregulated by proinflammatory cytokines including TNFα, IL-1β and IL-17. Conclusions According to our present data, glycosidases expressed by synovial membranes and ... MMPs, proinflammatory cytokines and chemokines [13]. There is increasing evidence that SFs are key players in the pathogenesis of RA by invading and eroding hyaline ca...

Ngày tải lên: 09/08/2014, 14:20

... symbol is not the most com- monly used symbol in the literature (for example, TP53 and P53). Finally, new online resources are continually being developed, and staying abreast of these tools and ... assays) https://products.appliedbiosystems.com/ab/en/US/adirect/ab?cmd=search&type=GEKEY&filter=GENE_SYMBOL&limit_to=ALL&species=H_SAPIENS,M_M USCULUS,R_norvegicus&keywor...

Ngày tải lên: 09/08/2014, 20:21

... Phusion polymerase (Finnzyme, Finland). PCR cycle condition was: 1 cycle at 98°C for 30 sec, followed by 30 cycles at 98°C f or 10 sec, 61°C for 30 sec, 72°C for 15 sec and a final cycle at 72°C ... spsim@fmhs.unimas.my Faculty of Medicine and Health Sciences, Universiti Malaysia Sarawak, Lot 77, Seksyen 22 KTLD, Jalan Tun Ahmad Zaidi Adruce, 93150 Kuching, Sarawak, Malaysia Yee and Sim...

Ngày tải lên: 10/08/2014, 05:21

... chromosome 7q36. Hepatic lipase (LIPC) is a lipolytic enzyme synthesized in hepatocytes playing a major role in HDL metabolism. It takes part in hydrolysis of triacylglycerides and phosphol- ipids of HDL 2 into ... events. Arterial Hypertension was evident at the time of primary surgery in 73% of our patients and did significantly Table 1: Demographics of patients and medical therapy a...

Ngày tải lên: 10/08/2014, 10:20