Báo cáo y học: "Common gene expression strategies revealed by genome-wide analysis in yeast" ppt

... comprehensive analysis of six variables characterizing gene expression in yeast, including transcription and translation, mRNA and protein amounts, reveals a general tendency for levels of mRNA and protein ... half-lives in the budding yeast proteome. Proc Natl Acad Sci USA 2006, 103:13004-13009. 10. MacKay VL, Li X, Flory MR, Turcott E, Law GL: Gene expression analyzed by hig...

Ngày tải lên: 14/08/2014, 08:20

... I/R injury [8,23]. Apart from chemokines also cytokines play a crucial role in mediating I/R injury after transplantation. An important group of pro-inflammatory cytokines is the interleukin-1 ... hypothermia [7]. However, not only locally generated inflammatory mediadtors like cytokines/chemokines [8] resulting from I/R injury but also systemic inflammatory mediators in the donor affect...

Ngày tải lên: 11/08/2014, 03:20

... that germination rate reflected the hybrid incompatibility of the alternate species. By using megagametophytes from a single tree in the hybrid zone, we built a nearly complete genetic map for pine ... megagametophytes w ere extracted a s described by Yin et al [27]. AFLP primer combinations were selected based on the results in Li et al [16]. AFLP genotyping protocol was described b...

Ngày tải lên: 12/08/2014, 03:21

... the interleukin 5 gene; λ Ig: the λ -chain of immunoglobulin gene; γIg: the γ-chain of immunoglobulin gene; IFN-ind: the interferon-inducible gene 6-16; IL4R: the interleukin 4 receptor gene; ... dehydrogenase subunit 4 gene, IL5 – the interleukin 5 gene, λ Ig – the λ -chain of immunoglobulin gene, γIg – the γ-chain of immunoglobulin gene, IFN-ind – the interferone-inducible...

Ngày tải lên: 02/11/2012, 11:08

... analyzed individually. Flow cytometry analysis The cell type composition and level of protein expression for some genes in the inguinal lymph nodes were analyzed by flow cytometry. Single cell ... information regarding the pathways in which the genes are operating. Conclusion We showed that a rather simple microarray analysis is useful in identifying interesting pathways and can...

Ngày tải lên: 09/08/2014, 01:23

... combining gene expression analysis with histomorphometry allows the exploration of any relationships between gene expression and indices of bone architecture and bone remodelling. Interestingly, ... matrix. Increased OPN may augment OB synthetic activity by increasing OB longevity and surface extent of bone formation. Hence, increased OPN mRNA expression in OA may contribute t...

Ngày tải lên: 09/08/2014, 08:23

... judged by genetic studies [27]. Interestingly, many of the candidate susceptibility genes for OA identified by genetic screening approaches have bone-related functions, further suggesting the involvement ... function and pathway analyses were carried out by searching the National Centre for Biotechnology Information database [52] and by using various analysis programs includ- ing On...

Ngày tải lên: 09/08/2014, 10:21

... include overactive inflammatory response in the form of hypercy- tokinemia (cytokine storm), that is initiated inside the infected cells or tissue in response to virus replication resulting in ... hours post infec- tion by employing gene expression profiling on a micro- array platform. A comparative analysis was thus carried out at different time points post infection between hi...

Ngày tải lên: 12/08/2014, 01:21

... <.05) within 21 days of pneumonectomy. Cluster analysis of the 22 genes indicated that changes in gene expression did not occur in a single phase, but in at least four waves of gene expression: ... was obtained using cell cycle flow cytometry of CD31 + endothelial cells (Figure 2). An early increase in S-phase cells was noted on Day 3 after pneumonectomy. By Day 6 after...

Ngày tải lên: 12/08/2014, 13:22

... inducing ubiquination of their receptors. Additionally, Smad7 can induce TGFβ-production by fibroblasts upon activation by pro-inflammatory cytokines like TNF and IFN-gamma, thereby enabling autocrine ... downregulation of decorin gene expression in COPD patients, at least partially via the Smad pathway. Our findings may explain why only a sub- set of smokers demonstrates an excess...

Ngày tải lên: 12/08/2014, 14:20