Báo cáo y học: ":Identification of novel stem cell markers using gap analysis of gene expression data" ppsx

... PHP. Abbreviations CYP, cytochrome P450 protein; Ebf, early B -cell factor; ESC, embryonic stem cell; GO, Gene Ontology; HSC, hematopoi- etic stem cell; mESC, murine embryonic stem cell; Nf2f, nuclear ... study we illustrate our methodology in the analysis of a database of stem- cell related DNA microarray samples that we previously developed (StemBase [7]). In particular...

Ngày tải lên: 14/08/2014, 08:20

... by PCR analysis. (b) q-PCR analysis and Luciferase assays using Dual Luciferase Reporter Assay System was performed on mES clones overex- pressing the firefly luciferase (Luc) gene. The system ... Reporter Assay System (Promega Italia, Milano, Italy). YFP fluorescence assay to detect the expression of the YFP reporter was performed using the DM6000 Leica Microscope. Karyotyping T...

Ngày tải lên: 09/08/2014, 20:22

... K, Fukada M, Kawai M, Yokochi T: Detection of lipopol- ysccharide (LPS) and identification of its serotype by an enzyme-linked immunosorbent assay (ELISA) using poly-L- lysine. J Immunol Methods ... Immunol Cell Biol 2004, 82(5):488-96. 12. Lim S -Y, Meyer M, Kjonaas RA, Ghosh SK: Phytol derivatives as novel adjuvants: 1. Assessment of safety and efficacy during stimulation of h...

Ngày tải lên: 11/08/2014, 10:23

... E.G7-Ova cells or 2C3 cells (1.2 × 10 6 cells/well) for 5 days in the presence of 10% CO 2 at 37°C to generate cytotoxic effector cells. Cytotoxicity assay As previously described, the target cells ... without any surfactant. Humoral immunity was assessed in terms of titer, specificity, and isotypic profiles. The effect on cell- mediated immunity was studied by assaying the induction...

Ngày tải lên: 11/08/2014, 10:23

... determine the promoter specif icity of the RNAP holoenzyme by binding to the core enzyme. Therefore, differential expression and/or activity of sigma factors can redirect global gene expression. During exponential ... 12:R17 http://genomebiology.com/2011/12/2/R17 Page 6 of 19 of (p)ppGpp in E. coli. Of the many detect ed (p)ppGpp targets that may contribute to the observed stre...

Ngày tải lên: 09/08/2014, 22:23

... (consisting of the number of procedures performed by sex, age-group and calendar year) was extracted individually and exported into Microsoft Excel (Microsoft Corp, Redmond USA) for analysis. MBS ... which are commonly documented for foot surgery but do not specifically pertain to the foot, the incidence of foot surgery reported here is an underestimate. Finally, analysis of the da...

Ngày tải lên: 10/08/2014, 21:23

... vivo identity of mesenchymal stem cells. Stem Cells 2008, 26:2287-2299. 3. Caplan AI, Bruder SP: Mesenchymal stem cells: building blocks for molecular medicine in the 21st century. Trends Mol ... marked protection of ischemic heart by Akt-modified mesenchymal stem cells. Nat Med 2005, 11:367-368. 51. Abdallah BM, Kassem M: Human mesenchymal stem cells: from basic biology to clin...

Ngày tải lên: 11/08/2014, 03:20

... quickly assessed when appropriate using the Glasgow Coma Scale. The degree of severity in critically ill patients was assessed using the Injury Severity Score (ISS) and Acute Physiology and Chronic ... Surgery Specialist, General Surgery II Service and Emergency Department (Surgical Section), Gregorio Marañón University General Hospital, Madrid, Spain 4 General Surgery Specialist, Gener...

Ngày tải lên: 12/08/2014, 20:20

... test this hypothesis. • Hyperoncotic albumin decreased the odds of acute kidney injury by 76% and of death by 48%. • Hyperoncotic hydroxyethyl starch increased the odds of acute kidney injury by 92% ... and generalizab ility of the CRYCO results with respect to hyperoncotic albumin, namely use of hyperoncotic albumin in a sm all minority of the most severely ill patients, concomit...

Ngày tải lên: 13/08/2014, 21:21