Báo cáo y học: "High-throughput analysis of spatio-temporal dynamics in Dictyostelium" docx
Báo cáo y học: "Functional analysis of an arthritogenic synovial fibroblast" docx
... histological analysis of injected joints revealed a
high incidence of disease transfer (Table 1), characterized
by variable degrees of synovitis, soft-tissue inflammation,
synovial hyperplasia, ... the
presence of the transgene could be detected in only the
injected knee. Careful analysis of the fibroblast-containing
organs did not show any evidence of tissue pathology; this...
Báo cáo y học: "Microarray analysis of gene expression in lupus" ppt
... attention on the interferon (IFN) pathway in
systemic lupus erythematosus (SLE) [3–6].
Analysis of microarray data
A statistical analysis of appropriately normalized micro-
array data is as important ... [18].
The microarray assay used (Panorama Cytokine Gene
Array membranes; Sigma Genosys, Inc) included
375 genes enriched in cytokines, chemokines, cell surface
286
ably derive fro...
Báo cáo y học: "Proteome analysis of bronchoalveolar lavage in Pulmonary Langerhans cell histiocytosis" pptx
... against this pro-inflammatory cytokine. Interestingly in our PLCH patients this
mechanism was down-regulated.
The results of our proteome analysis of PLCH BAL suggested the involvement of some ... phosphorylase, pyruvate kinase
isozymes, fibrinogen gamma chain, alpha 1B glycoprotein and actin cytoplasmic 1 were identified.
BAL proteome analysis of PLCH patients also revealed...
Báo cáo y học: "Stereological analysis of sciatic nerve in chickens following neonatal pinealectomy: an experimental study" pps
... the analysis of
hydroxyproline in biological tissues. Clin Biochem 1996, 29:225-229.
30. Edward CA, O'Brien WD: Modified assay for determination of
hydroxyproline in a tissue hydrolyzate. ... Embryology,
Ondokuz Mayıs University School of Medicine, Samsun, Turkey,
4
Department
of Anatomy, Ondokuz Mayıs University School of Medicine, Samsun, Turkey,
5
Ondokuz Mayıs Un...
Báo cáo y học: "Systematic analysis of transcribed loci in ENCODE regions using RACE sequencing reveals extensive transcription in the human genome" pptx
... for 16 novel isoforms.
We also analyzed expression of two gene loci, namely SYN3
and TIMP3, in cells in which their expression was not
detected by tiling microarray analysis. SYN3 and TIMP3 are
encoded ... T: DNA sequencing by indexer
walking. Clin Chem 2005, 51:1612-1618.
37. So AP, Turner RF, Haynes CA: Increasing the efficiency of SAGE
adaptor ligation by directed ligation chemist...
Báo cáo y học: "Microarray analysis of microRNA expression in the developing mammalian brain" ppt
... Quantification of
microarray data using three synthetic RNAs: syn1, syn2 and syn3. Each
data point is the average of two independent labelling/hybridization
reactions. Probes for the three synthetic ... temporal
expression of 44 microRNAs during mouse brain develop-
ment [39]. Their study used a dot-blot array approach and
direct labeling of microRNAs using radioactivity instead o...
Báo cáo y học: " Functional analysis of human and chimpanzee promoters" docx
... we find that in
five cases promoter activity differences are restricted to one of
the two cell lines, showing that interspecies differences in
promoter activity are often specific to cell line ... was used in a Dual-Luciferaser
Reporter Assay System (Promega) in a Wallac Victor 2 Lumi-
nometer (PerkinElmer). Promoter activity was measured by
normalizing the luciferase activity of...
Báo cáo y học: "Systematic analysis of gene expression in human brains before and after death" docx
... USA) microarrays (Table 1). Labeling of 1.2 µg of total
RNA, hybridization to Affymetrix
®
HG U133plus2 arrays,
staining, washing and array scanning were carried out follow-
ing Affymetrix
®
protocols. ... expression
patterns in postmortem human brains. Study of expression
levels of 14 genes in human brain autopsy and biopsy samples
found significant change in one of the gen...
Báo cáo y học: "Global analysis of mRNA stability in the archaeon Sulfolobus" pps
... longevity
observed in Sulfolobus.
In a genome-wide study in budding yeast [10], transcripts
involved in regulatory systems were suggested to decay rap-
idly. In contrast, we found many transcriptional ... extraction of stationary
phase genomic DNA, 200 ml cultures were grown in 1 l Erlen-
meyer flasks to an OD of 0.7.
Transcription inhibition by actinomycin D, and
sampling
Ac...
Báo cáo y học: "Proteomic analysis of mechanisms of hypoxia-induced apoptosis in trophoblastic cells"
... to identify key molecules in the course of hy-
poxia-induced apoptotic pathways. Such analysis of
trophoblastic cells will, in the near future, yield in-
sights into the mechanisms of preeclampsia ...
regulation of the JNK gene, but by modulating phos-
phorylation of JNKs. Thus, Bag-1 may inhibit apop-
tosis by suppressing the expression of Bid and Bad.
Bag-1 may also e...