Báo cáo y học: "GC- and AT-rich chromatin domains differ in conformation and histone modification status and are differentially modulated by Rpd3" doc

... Change in expression in ume6D Log(ume6Δ/WT) Rpd3p binding in wild-type and histone acetylation in rpd3 Δ cells in AT-rich and GC-rich isochorsFigure 6 Rpd3p binding in wild-type and histone ... levels and GC content of genes in yeast. GC-rich and AT-rich chromatin domains are marked by different levels of histone acetylation Histone modificati...

Ngày tải lên: 14/08/2014, 07:21

... detected by silver staining on polyacrylamid gel. Statistical Analysis Allele frequencies were determined by gene counting and compared using the א² test. The data was analysed using SPSS ... PCR condition were: 96°C for an initial 5 minutes (min.) followed by 96°C 1 min., 65°C 1 min., 72°C 1 min. for 43 cycles and final extension at 72° for 10 min. MS-PCR created two fragmen...

Ngày tải lên: 31/10/2012, 17:03

... chromatog- raphy, immunologically and by high-performance liquid chromatography. The biological activity of (2–5)A oligo- mers was verified by inhibition of the protein synthesis in rabbit reticulocyte lysate ... they contain proliferating cells and their interior is surrounded by an almost complete single-cellular layer of epithelial-like cells, pinacocytes; the cells inside the p...

Ngày tải lên: 21/02/2014, 15:20

... homology to previously characterized protein–protein interaction domains and no obvious simi- larity with known 3D structures as determined by SWISS - MODEL and 3 D - PSSM were detected. These analyses ... in both initiation and fork movement. In addition, MCM complexes bind with high affinity to core histone H3/H4 dimers [39,40] and to HBO1 [41], via distinct domains in...

Ngày tải lên: 17/03/2014, 10:20

... activated by thrombin, whereas PAR-2 is activated mainly by trypsin but also by mast cell tryptase. PARs are expressed by several cell types, includ- ing platelets and endothelial and inflammatory cells, ... human chondrocytes and that the levels are upregulated by the proin- flammatory cytokines IL-1β and TNF-α, an effect previously observed on chondrocytes by Xiang...

Ngày tải lên: 09/08/2014, 10:22

... colon (C)) and PBL (L) (indicated by different shapes and shading). RT sequences grouped by individual gut tissue and PBL to varying degrees in the different patients. Upon closer examination of ... patients and were involved in writing the paper. KN and KC were involved in designing the methods for collecting, analyzing and interpreting data, and helped putting the da...

Ngày tải lên: 13/08/2014, 01:20

... absolute insulin deficiency. Those children may derive clinical benefit from insulin replace- ment, analogous to using insulin to treat type 1 DM. Practition- ers may need to rethink insulin administration ... determine the aetiological cause of CIH in younger and middle-aged adults. Many factors in acute illness may cause beta-cell dysfunction, including elevations in pro-inflammator...

Ngày tải lên: 13/08/2014, 15:22

... an amino acid, p.Ser95del, in the elastin/laminin-binding domain of EBP. This variation i n the elastin-binding domain of the EBP protein may directly impair tropoe- lastin binding to EBP and ... GLB1 gene encoding EBP. The unique 32 amino acid region encoded by a frameshift in exon 5 contains an elastin/laminin-binding domain (underlined). The 3 bp in- frame deletion resulting in...

Ngày tải lên: 10/08/2014, 05:21

... being cleaved by trypsin at the N-terminus could not have been formed by trypsin cleavage at the C-terminus and there- fore represent ed the C-terminus of the mature NC sub- domain.CleavagebytheviralPRatthissitenotonly generates ... before starting t he analyses. The mass spectra were visualized and processed using FlexAnalysis software and sequence tag hints were obtained by ana-...

Ngày tải lên: 13/08/2014, 01:20

... melatonin in the routine care of critically ill patients, our findings need to be confirmed by a larger, possibly multicentre, randomised con- trolled trial, ideally using polysomnography as a continuous measure ... written informed consent. Patients were randomly assigned to melatonin or placebo by the pharmacy, using random assignment in blocks of four. Melatonin 10 mg, formulated...

Ngày tải lên: 13/08/2014, 10:20