Báo cáo y học: "Characterizing the expression of the human olfactory receptor gene family using a novel DNA microarray" pps

... hybridization quality, and for the normalization of the arrays (Additional data file 6). Hybridization and pre-processing of the data Hybridizations and scanning of the arrays were performed at the ... interactions information Open Access 2007Zhanget al.Volume 8, Issue 5, Article R86 Research Characterizing the expression of the human olfactory receptor gene f...

Ngày tải lên: 14/08/2014, 07:21

... were then washed in 1% BSA in PBS and a phycoeryth- Table 1 Characteristics of patients Patient Category Diagnosis RF Sex/age Duration of disease Therapy 1 Early RA RA - M/64 9 mo None 2 Early RA ... MTX 19 Late RA RA + F/72 44 yr Cyclosporine, NSAID 20 Late RA RA + F/59 18 yr Salazopyrin, Glucosamine 21 Late RA RA + M/73 20 yr Paracetamol 22 Late RA RA + F/59 11 yr MTX, Remicade 23 Lat...

Ngày tải lên: 09/08/2014, 10:20

... GAT GAG AGA GGC AGC TCC AG 3'; BCL 2A1 F 5' GCC ACC ATG ACA GAC TGT GAA TTT GGA TAT 3', BCL 2A1 R 5' TCA ACA GTA TTG CTT CAG GAG AGA 3': Primer pairs were designed using Primer3 ... study has clearly shown that SPLUNC1 is significantly increased in the small airways of patients with advanced CF. The spe- cificity of this observation is shown by the fact th...

Ngày tải lên: 12/08/2014, 15:21

... remove any genomic DNA that might contaminate the RNA prepa- rations. Complementary DNA (cDNA) was prepared using a synthesis kit (SuperScript II DNA Preamplification Sys- tem; Invitrogen). A cDNA ... International League of Associations for Rheumatology (ILAR) classification [8] and were managed at the Pediatric Rheumatology Unit of Padua University. The procedure was p...

Ngày tải lên: 09/08/2014, 06:22

... cytometer (Luton, UK). Statistical analysis All data were expressed as the mean of the individual experi- ments ± the standard error of the mean. Data were analysed using one-way or two-way analysis ... that cycles of hypoxia and reoxygenation within the synovium may also exacerbate complement activation. In situ analysis has demonstrated abundant local synthesis of C3, C3...

Ngày tải lên: 09/08/2014, 08:22

... this case, the feeling of a mass in the throat without dysphagia was the only symptom of large pyriform sinus lipoma. The preferred method of treatment for laryngeal and hypopharyngeal lipogenic ... feeling of a mass in the throat without dysphagia was the only symptom of large pyriform sinus lipoma. Although the mass may be asymptomatic, it should be surgica...

Ngày tải lên: 25/10/2012, 10:51

... By the age of 8 years, the top of the frontal sinuses is at the level of the orbital roof, and by the age of 10 years, the sinuses extend into the vertical portion of the frontal bone. The ... second year of life [4,8,9]. By the age of 4 years, the average cranial extent of the frontal sinus reaches half the height of the orbit and extends ju...

Ngày tải lên: 25/10/2012, 11:04

... paper [22]. The primers with the sequence 3’-GAGGAGACAGTCCTACTGAAA (API1) and 3’-CATAGCATTATCCTTCGGTTC (API2) were used to detect cIAP2. Primers with the sequence 3’-GGGAAGCAGAGATCATTTTGC (API3) ... was investigated. There are at least six Human apoptosis inhibitors (IAP) have been identified now. They are cIAP1, cIAP2, XIAP, NAPI, survivin and pIAP. Using gene- assay technolo...

Ngày tải lên: 02/11/2012, 11:17

... to the antibody [18]. Conventional pepscan analysis however, does not allow easy analysis of the contribution of the carbohydrate portion. To assess the involvement of carbohydrates in antibody ... Selective presat- uration of the protein was achieved by a train of 40 Gaussian shaped pulses of 50 ms length, each separated by a 1 ms delay, leading to a total saturat...

Ngày tải lên: 21/02/2014, 15:20

... Tewari, Y. B. & Goldberg, R.N. (2000) A thermo- dynamic study of the hydrolysis of 1-glutamine to (1-glutamate + ammonia) and of 1-asparagine to (1-aspartate + ammonia). J. Chem. Thermodynamics ... in the presence of GTP, UTP and the nonhydrolyzable ATP analog ADPNP [8]. Furthermore, it was shown that the fold activation of the glutaminase activity by GTP was simila...

Ngày tải lên: 21/02/2014, 15:20