Báo cáo y học: "A distance difference matrix approach to identifying transcription factors that regulate differential gene expression" pps

... difference matrix approach to identifying transcription factors that regulate differential gene expression Pieter De Bleser *†‡ , Bart Hooghe *†‡ , Dominique Vlieghe *†‡ and Frans van Roy †‡ Addresses: ... expression. Background Eukaryotic genes are transcriptionally regulated by the coor- dinated interaction of multiple transcription factors with arrays of transc...

Ngày tải lên: 14/08/2014, 07:21

... IB, CM, NW: protocol development, laboratory fingerprint generation. JB: end sequencing and analysis. RC, RC, MF: data analysis. DL: protocol develop- ment, laboratory fingerprint generation. TP: ... genomic heterogeneity, and the implica- tions of this heterogeneity to human phenotypic diversity and disease, have recently been widely recognized [1-5], energiz- ing efforts to develop cata...

Ngày tải lên: 14/08/2014, 08:20

... cerevisiae using a tiled microarray approach [7]. From this study, the concept that nucleosomes generally occupy fixed positions at genes took hold. High-resolution microarray approaches have now produced ... accessible or refractory. As a result, nucleosomes can regulate processes that require access to DNA, such as DNA replication and transcription [5]. In addition, many gene...

Ngày tải lên: 09/08/2014, 20:20

... and a pyrosequencing chemistry to gener- ate DNA sequence reads by synthesis [2]. Longer reads and shorter sequencing run times make the 454 platform a powerful tool for de novo assembly of small ... 11:R15 http://genomebiology.com/2010/11/2/R15 Page 2 of 9 samples to enable handling by automation; redesign of process steps to be amenable to automation, particularly sample cleanup and...

Ngày tải lên: 09/08/2014, 20:21

... database [GEO:GSE19836]). In order to identify downstream transcriptional effects of the 20 overexpressed genes, microarray data were ana- lyzed to detect differentially expressed genes (that is, in induced ... Down syndrome. Am J Hum Genet 2008, 83:388-400. 19. Masui S, Shimosato D, Toyooka Y, Yagi R, Takahashi K, Niwa H: An efficient system to establish multiple embryonic stem cell...

Ngày tải lên: 09/08/2014, 20:22

... other cytotoxic agents This analysis evaluated 23 trials (5,577 patients) compar- ing single-agent gemcitabine with gemcitabine-based combinations with other cytotoxic agents. For the pri- mary endpoint ... followed by weekly 250 mg/m 2 . Gem Gem 1,000 mg/m 2 weekly × 7 followed by 1-week rest, then weekly for 3 weeks, q4w Saif MW 2009 Gem/LY Gem 1000 mg/m 2 d 1,8,15 , q4w; continuously admin...

Ngày tải lên: 10/08/2014, 21:23

... by ortho-nitropheny l-D-galacto- pyranoside (ONPG) hydrolys is using b-Gal Assay kit (Promega). The cells were solubilized by scraping with 400 μl of cell lysis buffer of Lucif erase Assa y System ... fibrosis. The discrepancy between ours and the previously reported results may be due to the small study populatio n in the previous study[16] or to ethnicity differences between study coh...

Ngày tải lên: 12/08/2014, 13:22

... protocol, mice were treated by endo-tracheal delivery of combined 4B/4D + 7A AON daily from Day 1 to Day 5, then once a week on Day 8 and 15. On days 8 to 12 and 15 to 18, mice were nose-only ... admin- istered into the trachea using a microsprayer (Penn- Century). On days 6 to 9, other groups of mice were treated by gavage with vehicle (0.5% methylcellulose) or roflumilast (Rasayan)...

Ngày tải lên: 12/08/2014, 14:20

... contra-indication to manual therapy [7,8] (Table 1). Other (yellow flag) features of the clinical history may affect the outcome of manual therapy and therefore recov- ery [7,8] (Table 2). A differential ... Possible features that may affect manual therapy outcome and ultimate patient recovery for patients presenting with shoulder pain (yellow flags) [7,8]. YELLOW FLAGS Previous histo...

Ngày tải lên: 13/08/2014, 13:22

... initiation. They are also a tool for dynamic Table 1 Summary of selected factors potentially influencing the decision to initiate renal replacement therapy in critically ill patients Factors Patient-specific ... kidney dysfunction (that is, rhabdomyolysis, tumor lysis syndrome). Electrolyte distur- bances and metabolic acidosis induced by certain toxins can also be readily corrected wi...

Ngày tải lên: 13/08/2014, 19:20