... Research Center, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka, 565-0871, Japan 2. PRESTO, Japan Science and Technology Agency, 4-1-8 Honcho Kawaguchi, Saitama, ... Sigma Koki Co., Ltd., Ja-pan. The beam profile of this laser system was ob-served with a LEPAS-11 Laser Beam Profiler (Hamamatsu Photonics K.K., Japan). A diameter of circular beam was approximately ... increased laser en-ergy. The area expressing alkaline phosphatase (ALP) activity was stained (magnification: x50). Laser irradiated samples displayed immunopositive staining for osteocalcin, a...