Báo cáo y học: " Identification of metabolic system parameters using global optimization methods" ppt

... sorted cellsReanalysis of triple positive sorted cells. (a) Forward and side scatter characteristics of sorted osteoarthritic cartilage cells. (b-d) CD9-fluorescein isothiocyanate (FITC)/CD166-phycoerythrin ... split by trypsintreatment (0.05% trypsin, 0.02% EDTA; Biochrom) at 75%confluence.Flow cytometry analysis of cells Either isolated cells from OC were directly used for flowcytometric analysis ... specific staining. The distribution of OC cells in forward/side scatter exhib-ited no difference between isotype and antibody staining. Amaximum of 2% positive cells by staining with isotype anti-body...

... of the anti -SmD3 peptide (SMP) assayAssay performance characteristics of the anti -SmD3 peptide (SMP) assay. (a) Intra-assay and interassay variability, (b) linearity, and (c) receiver operating ... (Rheumaklinik Aachen, Aachen, Germany), Prof.Dr MJ Fritzler (University of Calgary, Calgary, Canada) andby Labor Limbach (Heidelberg, Germany).To assess further the assay specificity, we analyzed ... 1Research articleIdentification of a SmD3 epitope with a single symmetrical dimethylation of an arginine residue as a specific target of a subpopulation of anti-Sm antibodiesMichael Mahler1,...

... pathophysiology of endothelial cells, and antibody toendothelial cell antigen (AECA) has been reported. Reportson the prevalence of AECA have varied largely and alpha-eno-lase was reported as ... Reiter's syndrome, inflammatorybowel diseases etc. On the other hand, further analysis of theassociation of anti -kinectin antibody with different manifesta-tions or disease 'subtypes' ... 1). The 120 kDaantigen was also shown to migrate differently from alanyl tRNAsynthetase in another Western blot analysis (data not shown)and did not share any apparent crossreactive epitopes...

... implicates citrullinated α-enolase as a candidate autoantigen that could drive the chronic inflammatoryresponse in RA.Introduction Rheumatoid arthritis (RA) is a common and disabling diseaseaffecting ... Sugaya M, Hanagiri T, Yasumoto K: [Tumor marker in primary lung cancer]. J UOEH 2004, 26:473-479.38. Suzuki A, Yamada R, Chang X, Tokuhiro S, Sawada T, Suzuki M,Nagasaki M, Nakayama-Hamada M, ... immunoblotting. This suggests that citrullinated α-enolase is at least as immunodominant as citrullinated filag-grin or citrullinated vimentin, because, by immunoblotting, thefrequency of antibodies against...

... http://arthritis-research.com/content/8/4/R100Page 1 of 13(page number not for citation purposes)Vol 8 No 4Research article Identification of arthritis-related gene clusters by microarray analysis of two independent mouse models for rheumatoid ... arthritis.ConclusionWe have performed a comprehensive transcriptome analysis of two etiologically different RA models by using microarraysand identified many genes that were commonly activated inboth models. Because ... Discovery of distinctive gene expression profiles in rheumatoid synovium using cDNA microarray technology: evidence for the existence of multiplepathways of tissue destruction and repair. Genes...

... necrosis factor receptor-associated factor 6 5'-AGA CAA GAC CAT CAA ATC CGG GAG3'-TCC AGG GCT ATG AAT CAC AAC AGGc-Fos 5'-CAG GAG ACA GAC CAA CTA GA3'-TTC ACG GAC AGA TAA GGT ... Miyazaki T, Katagiri H, Kanegae Y, Takayanagi H, Sawada Y, Yamamoto A, Pando MP, Asano T, Verma IM, Oda H, et al.: Recip-rocal role of ERK and NF-κB pathways in survival and activa-tion of ... AAT GAG GTC ATC CCT3'-AGA CAC ATT GAC CAC AGA GGC ACTβ-Actin 5'-GTC CTC TCC CAA GTC CAC ACA3'-CTG GTC TCA AGT CAG TGT ACA GGT AAGAPDH 5'-TGA TGA CAT CAA GAA GGT GGT GAA G3'-TCC...

... markers of bone metabolismand are regulated in bone by pro-inflammatory cytokines.The objective of this study was to investigate the role of HNE in OA osteoblast metabolism by determining its ... demon-strated by inhibition of ALPase activity. ALPase participates in the differentiation of osteoblasts and provides phosphate forhydroxyapatite mineral formation. Its inhibition by HNE couldtherefore ... pg/ml, respectively.Protein detection by Western blotting Osteoblasts were incubated in fresh medium containing 0.5%FBS/HAMF12/DMEM in the presence of increasing concen-trations of HNE (0 to 20...

... methyla-tion is considered as a therapeutically relevant strategy forcancer treatment. Among many agents with DNA methy-lation-modifying capability, 5-aza-2’-deoxycytidine (decita-bine; 5-Aza) ... as demonstrated by the US Food and Drug Administration approval of the DNA methyltransferase inhibitors azacytidine and 5-aza-2’-deoxycytidine for the treatment of myelodysplastic syndromes. But ... Our data provide a proof -of- concept that procainamide could be pharmacologically exploited todevelop novel DNA methylation inhibitors, of which the translational potential in cancer therapy/prevention...

... Carroll et al.: Reliability of capturing foot parameters using digital scanning and the neutral suspension casting technique.Journal of Foot and Ankle Research 2011 4:9.Submit your next manuscript ... from the camera to the plantar surface of the foot of 400 mm. Sixplaster casts and six digital images were taken of the left foot of all participants. Three casts of the left foot weretaken by ... inter-rater reliability of digital scanning and the neutral suspension casting technique to measure six foot parameters. The neutral suspension casting technique is a commonly utilised method for...

... each of theseto acid reflux and aspiration. Acid reflux can certainlyacidify the airway, but it is just one of several pathwaysleading to airway acidification. Acids emanating from anylevel of ... this hypothesis by means of pharyngeal acid challenges. We then tested for sponta-neous hypopharyngeal gastric acid reflux by performingEBC pH testing in patients suspected of having acid reflux cough ... we tested the hypothesis that acid reflux to thelevel of the airway is a critical component for the trigger-ing of cough in acid reflux cough. Data are mixed about the utility of proton pump...

... cord injury and other inflammatory or destructiveconditions of the CNS[24,25].We investigated the cross packaging ability of the Gag-Polcomponents of HIV-1, HIV-2 and SIV and found a unique non -reciprocal ... 60% of glial cell expressing GFP with 20 ng of input vector and approximately 58% with 10 ng of vector. In summary, the gene transfer efficiency of the HIV-2 GFP vector to be cross packaged by SIV ... HIV-1 and HIV-2 homologous vector systems.Transduction of human embryonic neuronal stem cellswas also performed using the HIV-1 and HIV-2 homolo-gous vector system (not shown) and with the SIV...

... applicable to continuous rapidlychanging multivariate physiologic data in critically injured patients, yielding important insight into patientphysiology and outcomes. We define that at any time, thepatient ... provided the original work is properly cited.ResearchIdentification of complex metabolic states in critically injured patients using bioinformatic cluster analysisMitchell J Cohen*†1, Adam ... 14:R10http://ccforum.com/content/14/1/R10Page 5 of 11an internal control of the clustering process and indicateson a gross level that the clustering identifies meaningfulgroupings of physiology.Clinical evaluation of clustersWe...

... Comparative characterization of the fermentation pathway of Saccharomyces cerevisiae using biochemical systems theory and metabolic controlanalysis. Steady-state analysis. Math Biosc 1995, 130:51-69.28. ... casewith S-systems. GMA systems are also interesting in thatthey include both stoichiometric systems and S-systems asspecial cases so that they allow a seamless transition fromlinear to fully kinetic ... therefore focused on S -system models withinthe modeling framework of Biochemical Systems Theory(BST), which itself has a rich history of successful analysisand application. S-systems, and the alternative...